Method for observing microscopic ingestion dynamics mechanism of bees

Abstract

The invention discloses a method for observing a microscopic ingestion dynamics mechanism of bees. The bionic corolla feeder and the sample container are fixed on a microscope objective table through a fixator, the bee ingestion process is recorded through a high-speed camera installed on an optical microscope, and videos are analyzed. According to the invention, not only can the ingestion process of bees be observed, but also a micro-analysis method can be provided for the research on the ingestion mode of pollinating insects, a micro-dynamics analysis angle is provided for the coevolution research of hymenoptera insects and angiosperms, and a bionic idea is provided for the design of a micro-fluid transport structure.

Description

technical field [0001] The invention belongs to the field of biological observation. More specifically, it relates to a method for observing the microscopic feeding dynamics of honeybees. Background technique [0002] As one of the most important pollinators, bees have very important ecological value. During the process of pollinating plants, the residence time of bees is mainly affected by the efficiency of ingesting nectar. Compared with pollinators such as butterflies and moths with slender mouthparts, bees have a significant ability to ingest highly viscous nectar, which makes They have access to higher energy density liquid food. [0003] After tens of thousands of years of high evolution, honey-eating insects have developed mouthpart structures and feeding mechanisms that are highly adapted to various feeding conditions. In-depth research on the mechanism of bee drinking honey can not only improve people's understanding of the function of bee mouthparts, but also im...

AI Technical Summary

Problems solved by technology

[0005] This method of observing honey bees drinking honey has the following disadvantages: 1. The bees cannot move freely, and the abdomen will be oppressed in a small confined space. This passive honey drinking behavior is difficult to accurately reproduce the bees in the natural state. and the feeding ability will be greatly affected, which will lead to deviations in the data, and the narrow centrifuge tube will limit the bee's limb regulation behavior except the mouthparts, and it is impossible to update the many structural coordination adjustment mechanisms in the sample feeding process. In-depth observation and research; 2. When the bees are restricted by the fixer, they will unconditionally reflexively break free, and the probability of bees in the state of trying to break free will decrease actively eating sucrose solution (artificial nectar), thus greatly Reduce the efficiency of the experiment; 3. During the confinement process, there is a high probability that the important receptors of the bee's abdomen or head antennae will be damaged, which will not only affect the experimental data, but also increase the loss of the experimental bees, which violates the animal experiment 4. The volume of the centrifuge tube is fixed. If the size of the sample used in the experiment changes, or the species of insects to be clamped changes, the shape of the body changes, then the space of the centrifuge tube will be too small or the shape will not be correct. Appropriate results that lead to ineffective stationary sample activities

[0006] Chinese patent CN 105145410 A discloses a bee behavior observer and bee observation system, which uses a camera to observe the life behavior of bees. Although it can effectively observe the real living conditions of bees in the beehive in all directions, it cannot observe the microscopic feeding behavior of bees. At present There is no report on the observation method of microscopic feeding dynamics of bees

Images