Bactrocera minax sex pheromone, and extraction method and application thereof

Abstract

The invention relates to bactrocera minax sex pheromone, and an extraction method and an application thereof. A main active component of the bactrocera minax sex pheromone provided by the invention is undecanol. The extraction method of the bactrocera minax sex pheromone comprises the steps of immersing bactrocera minax sex pheromone in a mating season in ethyl ether for 20 s-300 s; analyzing by using a gas chromatography-mass spectrometer to obtain chemical components of an extract liquid; and determining activities of the chemical components in the extract liquid by using a Y-type olfactometer. The bactrocera minax sex pheromone provided by the invention can be used as a sex attractant for monitoring field occurrence conditions of the bactrocera minax and disturbing mating of the bactrocera minax by mating disruption, and can be used as an attractant to trap and kill the bactrocera minax. The extraction method of the bactrocera minax sex pheromone is simple in operations, high in reproducibility and small in required bactrocera minax amount. Besides, a preparation period is short and result reliability is high.

Description

technical field [0001] The invention relates to a sex pheromone and its extraction method and application, in particular to a citrus Bactrocera sex pheromone and its extraction method and application. Background technique [0002] Bactrocera citrus Bactrocera minax (Enderlein) belongs to Diptera (Diptera) Tephritidae (Tephritidae). It is a destructive pest of citrus. In my country, it is mainly distributed in Hunan, Hubei, Sichuan, Chongqing, Yunnan, Guizhou and other regions. It is only found in India and Bhutan abroad. reports. The worm produces one generation per year. It lays eggs in the citrus fruit. The larvae drill into the tangerine fruit and feed on the pulp of the tangerine petals. The fruit will turn yellow and fall off before it is ripe, and lose its edible value. The fruit loss rate is about 20-30%. When the yield loss is serious, it can reach 70%-80% or even 100%. It has become the number one pest in citrus production in my country, and the prevention and contr...

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Problems solved by technology

[0005] However, so far, there are only two reports about the sex pheromone of Bactrocera citrus in the 1990s, but neither of them has obtained the chemical composition of the sex pheromone: Lu Hongxue et al. used a vacuum pumping device to obtain citrus The pheromone extract released by the female adults of Bactrocera dorsalis was eluted with dichloroethane to obtain the pheromone extract. Through indoor bioassays, it was found that the extract had obvious sexual excitement stimulation responses to male adults of Bactrocera citrus, such as Swinging antennae, fluttering wings frequently, tilting the abdomen, stretching out the gripper, etc., but the article did not report the active chemical components of its sex pheromones ("Extraction and testing of sex pheromones in Bactrocera citrus", Lu Hongxue et al. , Journal of Hubei Agricultural University, Vol. 18, No. 1, pp. 32-34, March 1998); Xia Zhongying et al. (1997) used the improved Wang Liangchen Yamamoto method to collect volatile Substance, using wind tunnel instrument to test its attracting effect, found that the chemical substances released by female insects have obvious attracting effect on male insects, but the 2,6-di-tert-butyl isolated and purified from the collected volatiles released by female insects When conducting field experiments with a small amount of cresol crystals, many Bactrocera citrus flies were found flying around the trap, but they did not enter the trap, so this study still failed to confirm the sex pheromone composition of Bactrocera citrus (" Preliminary report on the study of sex pheromone of Bactrocera citrus, Hubei College of Traditional Chinese Medicine, Xia Zhongying, etc., Chinese Materia Medica Vol. 20, No. 10, pp. 492-493, October 1997)

[0006] In a word, although there are sporadic reports about the sex pheromone of Bactrocera citrus, none of them has actually prepared the chemical components of the sex pheromone, and the preparation method is complicated and requires a large amount of insects

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