117 results about "Pheromone signaling" patented technology

The invention discloses a pheromone binding protein derived peptide capable of effectively monitoring sex pheromones of fall webworms and a biosensor, and belongs to the field of biosensors. At present, methods such as sample plot survey and sex traps commonly used in fall webworm monitoring have the problems of poor timeliness, high labor cost, insufficient data continuity and the like. In order to solve the problem, the pheromone binding protein-derived peptide provided by the invention has an amino acid sequence as shown in SEQ ID NO: 2, the biosensor comprises a substrate and an interdigital electrode, a single-walled carbon nanotube is attached to the electrode, and the electrode is also connected with the pheromone binding protein-derived peptide. The sensor can specifically detect sex pheromones of fall webworms, has no response to 17 plant volatile matters, and can detect sex pheromones released by as low as five live female fall webworms under laboratory conditions. The method has application potential in the fields of early pest monitoring, prevention and control efficiency improvement, ecological safety guarantee and the like.

The invention discloses an ostrinia furnacalis sex attractant and a preparation method and application thereof.The sex attractant is prepared from sex pheromone and a solvent, and the sex pheromone comprises cis-12-tetradecene acetate, trans-12-tetradecene acetate and tetradecene acetate; the mass ratio of the cis-12-tetradecene acetate to the trans-12-tetradecene acetate is 3: (1-2), and the ratio of the mass sum of the cis-12-tetradecene acetate and the trans-12-tetradecene acetate to the mass of the tetradecene acetate is 3: (0.8-1.2). The sex attractant provided by the invention can significantly increase the trapping amount of Asiatic corn borers, has good stability and accuracy, and can be used for monitoring, early warning, trapping, killing and preventing Asiatic corn borers in South China.

The invention relates to the technical field of genetic breeding, and discloses a method for stabilizing the chemical property of a subtropical silkworm mid-line variety, which comprises the following steps: screening an egg circle with the optimal chemical property from a production group to establish an initial group; second-generation frame seed production is obtained through incubation and single moth breeding in combination with comprehensive screening of pupa unified life rate, cocoon quality and offspring hatching rate; single moth breeding screening is repeated twice, and third-generation and fourth-generation frame seed production is obtained; adopting a 4 / 4 moth area mixed breeding method to obtain five-generation frame seed production; then five-generation frame seed production is used as a new starting point, the breeding and screening processes are repeatedly executed, each time 4 / 4 moth area mixed breeding is executed as a breeding cycle, and at least two breeding cycles are continuously executed until a new silkworm strain with the sum of the seed generation rate and the re-egg-out rate stably lower than 0.5% is obtained. The method is used for cultivating new silkworm strains which are stable in chemical property and maintain excellent economic characters and robustness, and provides support for stable development of the silkworm industry.

The invention relates to the field of insect trapping, in particular to a tomato leaf miner ultraviolet light and sex pheromone synergistic interaction trapping system and application. The method comprises the following step: under a facility cultivation condition, directly applying an ultraviolet lamp and a tomato leaf miner sex lure on the ground. Three different types of traps including a sex trap, an ultraviolet light trap and a synergistic interaction trapping system are directly placed on the ground under the facility cultivation condition, and male and female tomato leaf miner have the strongest tropism on the synergistic interaction trapping system. The daily average total moth trapping amount of male and female insects, the moth trapping amount of male insects and the moth trapping amount of female insects and egg-carrying female insects are all the highest; and in the trapping period of 108 days, the moth trapping efficiency of the synergistic interaction trapping system is always superior, and the ecological safety is also considered while the trapping efficiency is remarkably improved.

The invention discloses an odor receptor gene SinfOR21 of sesamia inferens and application of the odor receptor gene SinfOR21. Belongs to the technical field of biology. According to the invention, sex pheromones of Zhejiang and Guangdong sesamia inferens strains and expression and functional verification of olfaction receptor genes of the sesamia inferens are identified and compared. The method comprises the following steps: firstly, identifying pheromone mixtures with region specificity, and verifying the behavior effectiveness of the pheromone mixtures in field trials; through transcriptome and function analysis, it is proved that a SinfOR21 receptor of the Zhejiang strain is specifically combined with Z11-16: OH, and a homologous receptor of the Guangdong strain does not have the function. And determining that the sex pheromone mixture of the sesamia inferens should be composed of Z11-16: Ac, 16: Ac and Z11-16: Ald. Different from reported sex pheromone mixtures of sesamia zhejiangensis, the sex pheromone mixture of sesamia zhejiangensis is composed of Z11-16: Ac, Z11-16: OH and Z11-16: Ald.

The present application belongs to the technical field of fruit fly prevention and control, and discloses a disorienting agent, a preparation method and application thereof. The disorienting agent contains rosemary extract and orange peel essential oil. The rosemary extract and orange peel essential oil contain terpenes and other compounds (such as 1,8-cineole in rosemary), which can compete with natural signal molecules to bind to odor binding proteins or olfactory receptors, which can cause fruit flies to fail to activate the correct nerve signal or directly block the signal transduction pathway. The orange peel essential oil in the disorienting agent has a strong smell, which can strongly cover or mask the weak smell signal emitted by the fruit, forming an "olfactory masking" effect, so that the fruit fly cannot find the fruit. Moreover, compared with sex pheromones as disorienting agents, which can only act on male fruit flies, the disorienting agent of the present application can interfere with the olfactory receptors of adult fruit flies to make them unable to find the target, so that they cannot accurately locate the oviposition site and harm the fruit. The disorienting agent is effective for both female and male fruit flies.

The invention belongs to the technical field of ecological prevention and control of agricultural pests, and relates to a peach fruit moth sex pheromone communication interference composition derived from host plants. The interferent is selected from at least one of trans-2-heptanal, trans-2-hexenyl acetate, leaf acetate and octanoic acid. The interferent can be used for predicting, forecasting, preventing and controlling peach fruit moths. The communication interferent provided by the invention can influence chemical communication between female and male imagoes of the peach fruit moth. The method can be combined with sex pheromones to be used for predicting, forecasting, preventing and controlling the peach fruit moths, and a new combination is provided for ecological prevention and control of the peach fruit moths.

The invention discloses a preparation method and application of a cnaphalocrocis medinalis pheromone microcapsule, and belongs to the technical field of cnaphalocrocis medinalis pheromone embedding and slow release. The microcapsule comprises a microcapsule wall material and a microcapsule core material, the microcapsule wall material comprises gelatin and Arabic gum; the microcapsule core material comprises cnaphalocrocis medinalis sex pheromone and liquid paraffin; the rice leaf roller sex pheromone is composed of (Z)-11-octadecenal, (Z)-13-octadecenal, (Z)-11-octadecenol and (Z)-13-octadecenol, and the mass ratio of the (Z)-11-octadecenal to the (Z)-13-octadecenol to the (Z)-13-octadecenol is (1-2): (10-15): (2-3): (4-6). The cnaphalocrocis medinalis sex pheromone microcapsule has the advantages of being sensitive, specific, efficient, long in half-life period, high in bioavailability, environmentally friendly and the like, can be used for trapping and killing cnaphalocrocis medinalis and preventing and treating isotropic interference, and has very high economic value and ecological benefits for agricultural production.

This invention discloses a sex pheromone antagonist for the poplar leafminer moth and its application. The active ingredient of the sex pheromone antagonist is cis-13-octadecene-15-ynyl alcohol. The sex pheromone antagonist of this invention can interfere with the orientation of male poplar leafminer moths towards females, causing males to become disoriented, thereby reducing the probability of encounters between males and females, interfering with mating, reducing the reproductive rate, and gradually controlling the extinction of the pest population, thus achieving the purpose of pest control. This invention can significantly reduce the damage caused by the poplar leafminer moth and is also environmentally friendly.

The application relates to the technical field of insect sex pheromone, and particularly discloses a method for controlling the reproduction of anisea lineata by using sex pheromone. The method comprises the following steps: S1, cultivating an insect source; S2, distinguishing male and female pupae; S3, identifying sex pheromone; S4, manufacturing an attractant core; S5, manufacturing a trap; and S6, matching the attractant core with the trap. The application has the advantages of reducing environmental pollution and green biological control.

The application discloses an information pheromone binding protein derivative peptide and a biosensor capable of effectively monitoring the sex pheromone of Hyphantria cunea, and belongs to the field of biosensors. At present, the monitoring of H. cunea is usually achieved by means of sample plot investigation, sex lures and other means, and problems such as poor timeliness, high labor cost and insufficient data continuity exist. In order to solve the above problems, the information pheromone binding protein derivative peptide provided by the application has an amino acid sequence as shown in SEQ ID NO: 2, the biosensor comprises a substrate and an interdigital electrode, single-walled carbon nanotubes are attached to the electrode, and the information pheromone binding protein derivative peptide is further connected. The sensor can specifically detect the sex pheromone of H. cunea and has no response to 17 kinds of plant volatiles, and can detect the sex pheromone released by as low as five live female H. cunea under laboratory conditions. The sensor has application potential in the fields of early-stage pest monitoring, improvement of prevention and control efficiency and guarantee of ecological safety.

The invention belongs to the technical field of insect sex pheromone preparation, and discloses a sphaerotheca hominis attractant and application thereof, the attractant comprises cis-11-hexadecenal and cis-9-tetradecene-3-methyl-2-butenoic acid ester, and the mass ratio of the cis-11-hexadecenal to the cis-9-tetradecene-3-methyl-2-butenoic acid ester is 2: 1. The hylocereus undulatus attractant provided by the invention is simple to prepare, convenient to use, very strong in trapping capability and good in stability; the method is high in specificity, free of resistance, harmless to natural enemies, non-toxic to people and livestock and environmentally friendly; the lasting period is long, and the control effect can be maintained for more than 30 days. The hylocereus undulatus attractant can be applied to insect situation monitoring, mass trapping and mating interference of hylocereus undulatus, and has important significance on comprehensive treatment of hylocereus undulatus.

The artificial efficient breeding method comprises the following steps that S1, host plants are planted in a spawning room, and the host plants are gramineae plants or asparagus plants which are easy to plant and have developed root systems; s2, collecting mature golden cicada nymphs which come out of the ground and are not subjected to eclosion, putting the collected mature golden cicada nymphs into an artificial climate box for artificial eclosion, and after eclosion and bee emergence, selecting parent adults; s3, putting the adults into a mating cage of an oviposition room according to a male-female ratio for mating, wherein an adult luring device consisting of a male cicada chirp generating device and a sex pheromone trap is arranged in the oviposition room. By arranging the adult luring device composed of the male cicada chirp generating device and the sex pheromone trap, the mating rate of the male cicada and the female cicada is synergistically increased, and technical support is provided for subsequently increasing the breeding rate of a cicada provenance; by means of the breeding method, efficient artificial breeding of the golden cicada seed source is achieved, and a seed source resource is provided for large-scale breeding of the golden cicada.

The invention discloses a sex attractant for yellow rice borers as well as a preparation method and application of the sex attractant, and relates to the technical field of agricultural pest monitoring, prevention and control. The sex attractant comprises an active component which is prepared from (Z)-dodecyl-9-ene-1-yl acetate, (E)-dodecyl-9, 11-diene-1-yl acetate and (7E, 9Z)-dodecyl-7, 9-diene-1-yl acetate, and the sex attractant is prepared from an active component which is prepared from the following components of (Z)-dodecyl-9-ene-1-yl acetate, (E)-dodecyl-9, 11-diene-1-yl acetate and (7E, 9Z)- According to the sex attractant disclosed by the invention, aiming at the current sugarcane area dominant population, the three active components have a synergistic effect, so that the trapping activity of the current sugarcane area dominant population is remarkably improved, and the problems that the trapping activity is low and the specificity is poor due to the fact that a control object is not matched with the dominant population in the existing sex attractant product are solved. Field trials show that the trapping quantity of the sex attractant provided by the invention on the yellow rice borers is obviously higher than that of a control group, the sex attractant has extremely high population specificity, and accurate monitoring and efficient trapping and killing of yellow rice borer populations can be realized.

The present application relates to the cedar moth sex pheromone, attractant and its application, the sex pheromone component mainly includes anti-9-21 carbon alkene, can attract to cedar moth adult in large quantities, especially with anti-9-21 carbon alkene, anti-7-9-21 carbon diene, 9-8-10-21 carbon diene and anti-6-8-21 carbon diene the sex pheromone attractant trapping effect most outstanding with mass ratio 1:1:0.25:0.25 composition.The sex pheromone and attractant prepared by it can be used for the insect situation of cedar moth forecast and large-scale trapping, and is environment-friendly, safe, has significant ecological benefits and economic benefits.

The invention relates to the field of biopesticides, in particular to a method for synthesizing sex pheromone of pinus tabulaeformis, which comprises the following steps: reacting 1-bromo-n-heptane (3) serving as an initial raw material with magnesium, and then reacting with (R)-epoxypropane (4) to obtain chiral alcohol 5; then reacting with paratoluensulfonyl chloride, and then reacting with dimethyl malonate to obtain malonate 7; carrying out sodium hydroxide catalytic hydrolysis and high-temperature decarboxylation to obtain an acid 8, and carrying out lithium aluminum hydride reduction and NBS bromination to obtain a chiral bromide 10; reacting with magnesium, and reacting with sulfonate 12 to obtain silyl ether 11; then carrying out TBAF deprotection to obtain chiral alcohol 13; and finally, reacting the chiral alcohol 13 with acetic acid to obtain acetic acid (2S, 3S, 7S)-3, 7-dimethyl-2-pentadecanol ester (1), and reacting the chiral alcohol 13 with propionic acid to obtain propionic acid (2S, 3S, 7S)-3, 7-dimethyl-2-pentadecanol ester (2). The method has the advantages of high total yield, low synthesis cost and the like.

The present application belongs to the technical field of insect control, and particularly relates to a Cydia funebrana sex attractant and a preparation method thereof. The present application aims to provide a new option for controlling Cydia funebrana. The technical scheme of the present application is a Cydia funebrana sex attractant, which comprises sex pheromone, synergist and solvent, the ratio of the sex pheromone to the synergist is 100:10-30; the solvent is at least one of n-heptane, n-hexane, silicone oil and paraffin oil; the sex pheromone comprises (Z)-8-dodecene acetate (Z8-12:Ac) and (E)-8-dodecene acetate (E8-12:Ac), and the mass ratio of the two is (85-90):(15-10); and the synergist is n-tetradecyl acetate (N-14:Ac). The present application provides a brand new Cydia funebrana sex attractant, which can be used for efficiently trapping Cydia funebrana.

1. The name of the design product: insect sex pheromone automatic monitoring and collecting system. 2. The use of the design product: for information collection and situation monitoring of insects. 3. The design points of the design product: in shape. 4. The picture or photo that best indicates the design points: perspective view.

The application discloses a termite sex pheromone attracting type termite control method and an attractor, and relates to the preparation of sex pheromone, wherein pheromone, aldehydes, olefins, alcohols and terpenes are mixed with organic solvents in a certain proportion; the mixed solvent is coated on moist solid materials and in the attractor assembly; the solid materials are placed on the fixed frame in the attractor and are buried in the area where termites often appear. The sex pheromone can be prepared simply, and the cooperation of the sex pheromone, the attractor assembly and the feeding assembly can automatically add the sex pheromone and the pesticide powder in the process of attracting termites, so that the efficiency of killing termites is greatly improved.

The application belongs to the technical field of biology, and particularly relates to a dsRNA of a target Helicoverpa armigera HaPburs gene and application thereof in prevention and treatment of the Helicoverpa armigera. HaPburs RNA interference mediated knockdown reduces the transcript level by 60.01%, and induces morphological abnormalities of pheromone glands (PG), which are irregular black protrusions; the silencing HaPburs reduces the main pheromone component Z 11-16: Ald content, while the male insect attraction rate and mating rate, cumulative egg production also significantly decrease, and the transcript level of the sex pheromone biosynthesis related genes HaPKA and HaACC is also significantly reduced. It is shown that HaPburs the genes participate in PG morphogenesis and regulate sex pheromone biosynthesis through a cAMP-PKA-ACC regulatory axis, thereby affecting the reproductive communication of the Helicoverpa armigera. The application provides a novel target and technical scheme for green and efficient prevention and control of the Helicoverpa armigera, and has important research and development value and application prospect.

The invention relates to the technical field of genes, in particular to cloning and application of a spodoptera frugiperda sex pheromone precursor synthetase fatty acyl-CoA reductase SfruFAR1 gene. The research finds that the SfruFAR1 gene is specifically and highly expressed in the insect sex pheromone gland. Through heterologous expression in a yeast system and assisted by enzyme activity analysis and GC-MS identification, it is proved that the SfruFAR1 protein has the biological activity of catalyzing the reduction of multiple fatty acyl-CoA with different carbon chain lengths into corresponding fatty alcohol. Experimental results show that the SfruFAR1 protein plays a core role in a process of synthesizing a precursor of the lepidoptera insect sex pheromone and fatty alcohol which is a component of the precursor. According to the invention, a molecular foundation is laid for realizing in-vitro efficient biosynthesis of the lepidoptera insect sex pheromone by using the SfruFAR1 gene, and a key functional gene tool is provided.

The invention relates to a sex pheromone of eutrophia maca and application of the sex pheromone. The sex pheromone of the eupolymyza cochinchinensis is 9, 12, 15-octadecatrienoic acid ethyl ester, and the sex pheromone of the eupolymyza cochinchinensis can be used for inducing male adults of the eupolymyza cochinchinensis or increasing the mating rate of the male adults and female adults of the eupolymyza cochinchinensis.

The application discloses an odor receptor derivative peptide capable of effectively monitoring sex pheromone of Spodoptera frugiperda and a biosensor, and belongs to the field of biosensors. At present, the monitoring of Spodoptera frugiperda is usually performed by means of sample plot investigation, sex lures and the like, and problems of poor timeliness, high labor cost and insufficient data continuity exist. In order to solve the above problems, the pheromone binding protein derivative peptide provided by the application has an amino acid sequence as shown in SEQ ID NO: 5, the biosensor comprises a substrate and an interdigital electrode, single-walled carbon nanotubes are attached to the electrode, and the odor receptor derivative peptide is further connected. The sensor can specifically detect the sex pheromone of Spodoptera frugiperda and has no response to 17 kinds of plant volatiles, and can detect the sex pheromone released by as low as two live female Spodoptera frugiperda under laboratory conditions. The sensor has application potential in the fields of early-stage pest monitoring, improvement of prevention and control efficiency and guarantee of ecological safety.

The invention provides a farmland comprehensive planting method based on crop diversity and an ecological buffer zone, which comprises the following steps: firstly, constructing a crop strip relay intercropping and multi-variety mixed sowing system, carrying out intercropping or relay intercropping and inter-variety mixed sowing on different crops in a farmland, and then, constructing a layered vegetation and filter material coupled farmland ecological buffer zone, according to the method, ecological buffer zones of arbor, shrub and herbaceous composite vegetation are constructed on the boundary of a farmland or the two sides of a ditch, then ecological prevention and control measures of double-lamp double-wave-band trap lamps and natural enemy release are deployed, and by combining sex pheromone trapping, measures of release of natural enemy including trichogramma, biological preparation and botanical pesticide spraying and frequency vibration type insecticidal lamps, the natural enemy release and the natural enemy release are combined, so that the ecological buffer zones of the arbor, the shrub and the herbaceous composite vegetation are obtained. Finally, filter material recycling and farmland soil improvement measures are carried out, organic fertilizer and microbial agents are combined to be applied to soil, the use of chemical herbicides is reduced in a mechanical weeding mode, and in a word, the method has the advantages of being environmentally friendly, increasing and stabilizing the yield and not damaging the ecological environment.

The invention is applicable to the technical field of trapping equipment, and provides equipment and a method for trapping phyllocnistis polycaryophyllata. The top of the collecting cylinder is screwed with a trapping part; the trapping part comprises an upper semi-spherical shell and a lower semi-spherical shell which are in threaded connection with each other; the top of the upper hemispherical shell is threadedly connected with a lure sleeve with a lure in a penetrating manner; a plurality of vent holes are uniformly formed in the outer wall of the lure sleeve; a plurality of screw holes are uniformly formed in the outer walls of the upper hemispherical shell and the lower hemispherical shell; a plurality of ventilation holes are uniformly formed in the outer walls of the upper hemispherical shell and the lower hemispherical shell; the screw hole is screwed with a branch pipe; according to the device, the branch pipes with the gradually-contracted elastic strips are arranged on the upper hemisphere shell and the lower hemisphere shell, a one-way entering channel is formed, injurious insects are prone to entering and difficult to exit, the evenly-arranged ventilation holes and lure sleeve ventilation holes are matched, sex pheromones are evenly diffused, and therefore the sex pheromones are evenly dispersed. The trapping coverage range and the trapping success rate are obviously improved.

A method for synthesizing the tomato leafminer sex pheromone (3E,8Z)-3,8-tetradecadienyl acetate. The present invention uses 3-butyn-1-alcohol as a starting material, and obtains a target fragment A through hydroxyl protection, Grignard reaction, reduction and acetylation; the protected 3-butyn-1-alcohol is subjected to bromination reaction, cross-coupling reaction of alkynyl bromide and Grignard reagent, reduction, deprotection and halogenation to obtain a target fragment B; the Grignard reagents of target fragments A and B undergo a Grignard reagent coupling reaction under the catalysis of Li2CuCl4 to obtain a key intermediate. Finally, the target product, tomato leafminer sex pheromone (3E,8Z)-3,8-tetradecadienyl acetate, is obtained through deprotection and acetylation. The raw materials of the present invention are cheap and easily available, all reaction conditions are mild, it is easy to scale up, there is little pollution to the environment, and promotion has good economic and social value.

The invention provides an olefine aldehyde intermediate and a synthetic method thereof, and a synthetic method of conopomorpha sinensis sex pheromone, and belongs to the field of compound synthesis. According to the synthesis method of the olefine aldehyde intermediate, provided by the invention, 1, 5-pentanediol is taken as a raw material, and the high-yield olefine aldehyde intermediate is obtained through transesterification, first oxidation reaction, Wittig reaction and Sagsa oxidation reaction; the yield of the olefine aldehyde intermediate can be further improved by limiting a catalytic system and an auxiliary agent of the Sagsa oxidation reaction. Results of the embodiment show that the synthesis method provided by the invention does not adopt alkyne compounds, the yield of the product in the Sagassa oxidation reaction stage can reach 81%, the total yield of the obtained olefine aldehyde intermediate can reach 48.5%, the sex pheromone of conopomorpha sinensis prepared by adopting the olefine aldehyde intermediate can reach 39.8%, and the yield is high.

The application belongs to the field of pesticides, and particularly relates to an attractant composition for preventing and treating Spodoptera frugiperda. The attractant composition for preventing and treating Spodoptera frugiperda is composed of the following components in parts by weight: sex pheromone 6-19 parts by weight and plant volatile 300-900 parts by weight; wherein the sex pheromone is composed of cis-9-tetradecenyl acetate, 7-dodecene acetate and cis-11-hexadecenyl acetate; and the plant volatile is composed of ethyl acetate, ethyl linalyl acetate and limonene. The attractant composition of the application is high in safety, free of any toxic side effects, easy to degrade, good in the prevention and treatment effect on Spodoptera frugiperda, reduces the use amount of chemical pesticides, reduces the pesticide residue of fruits, and reduces the impact on the ecological environment.

The invention belongs to the field of agricultural pest control, and provides a lure for luring grapholitha molesta male and female insects and peach fruit molesta male insects and application of the lure, and the lure contains grapholitha molesta sex pheromone, peach fruit molesta sex pheromone and peach and pear volatile matter. In addition to male grapholitha molesta and grapholitha peach molesta, the lure disclosed by the invention also has a relatively strong capability of attracting female grapholitha molesta, the trapping capability of the sex lure can be effectively improved, and the blank that the sex lure cannot attract female adults is filled. The product has the advantages of low cost, high efficiency, greenness and the like, and has wide application prospects.

The invention discloses a tobacco storage insect comprehensive prevention and control method based on a pheromone trap, and belongs to the technical field of tobacco storage insect prevention and control. According to the method, a composite pheromone formula is utilized, sex pheromones and aggregation pheromones of lasioderma serricorne and tobacco powder borers are contained, and the sex pheromones and the aggregation pheromones are loaded on PVA microspheres to prepare An LED photocatalytic plate is arranged in the intelligent trap, and yellow plate monitoring and plant source insect repellant are combined. Nanometer TiO is added into the trapping core to prolong the validity period, and the trap is powered by solar energy and is integrated with a temperature and humidity sensor. The pesticide has the advantages of dual action mechanisms, precise slow release, environmental protection, intelligent monitoring and the like, can improve the control effect, reduces the use of chemical agents, reduces the cost, and is suitable for tobacco storage enterprises.