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Method for producing hydrolyzed brain protein powder and cephalin by grease removal of supercritical carbon dioxide

A technology of carbon dioxide and cerebral protein powder, which is applied in the directions of edible phospholipid composition, protein food processing, animal protein processing, etc., can solve the problem of no simultaneous extraction and the like

Inactive Publication Date: 2012-11-14
LINGAO ZESHITAI BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] On the China Patent Inquiry Network, no patent has been found on the use of supercritical carbon dioxide extraction to remove lipids in brain tissue; and there is no use of supercritical carbon dioxide extraction to remove lipids in brain tissue , and then enzymatically extract the patent of hydrolyzed cerebroprotein powder, and there is no patent for simultaneous extraction of hydrolyzed cerebroprotein powder and cephalin in brain tissue

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment example 1

[0015] Remove the meninges and blood vessels from fresh or frozen animal brains, squeeze them through a 6-mesh sieve, spread them flat on a drying tray in a vacuum drying oven, and dry them at a vacuum degree of -0.08 Mpa and a temperature of 45°C until the water content is below 3%. Dried products, stored at -5°C for later use;

[0016] Fill the dried animal brain powder in batches with HA221-40-100 supercritical CO 2 In the extraction kettle of the extraction equipment, the pressure of the extraction kettle is 32.5 Mpa and the temperature is 41°C; the pressure of the separation kettle I is 18 Mpa and the temperature is 45°C; the pressure of the separation kettle II is 6 Mpa and the temperature is 48°C; the entrainer is 95% ethanol and the dosage is brain dry 20% of the product; extraction time 2 hours; carbon dioxide flow rate 60 liters / kg / hour; take out the residue in the extraction kettle for enzymatic hydrolysis to produce hydrolyzed cerebroprotein powder, take out the pa...

Embodiment example 2

[0024] Remove the meninges and blood vessels from fresh or frozen animal brains, squeeze them through a 6-mesh sieve, spread them flat on a drying tray in a vacuum drying oven, and dry them at a vacuum degree of -0.08 Mpa and a temperature of 45°C until the moisture content is below 3%. Dried products, stored at -5°C for later use;

[0025] Fill the dried animal brain powder in batches with HA221-40-100 supercritical CO 2 In the extraction kettle of the extraction equipment, the pressure of the extraction kettle is 31.5 Mpa and the temperature is 40°C; the pressure of the separation kettle I is 20 Mpa and the temperature is 46°C; the pressure of the separation kettle II is 5 Mpa and the temperature is 48°C; 25% of the product; extraction time 2 hours; carbon dioxide flow rate 60 liters / kg / hour; take out the residue in the extraction kettle for enzymatic hydrolysis to produce hydrolyzed cerebroprotein powder, take out the paste in the extraction kettle Ⅰ for extracting brain pr...

Embodiment example 3

[0033] Remove the meninges and blood vessels from fresh or frozen animal brains, squeeze them through a 6-mesh sieve, spread them flat on a drying tray in a vacuum drying oven, and dry them at a vacuum degree of -0.08 Mpa and a temperature of 45°C until the water content is below 3%. Dried products, stored at -5°C for later use;

[0034] Fill the dried animal brain powder in batches into HA221-40-100 supercritical CO 2 In the extraction kettle of the extraction equipment, the pressure of the extraction kettle is 32.0 Mpa and the temperature is 41°C; the pressure of the separation kettle I is 17 Mpa and the temperature is 46°C; the pressure of the separation kettle II is 5 Mpa and the temperature is 48°C; 23% of the product; extraction time 1.6 hours; carbon dioxide flow rate 70 liters / kg / hour; take out the residue in the extraction kettle for enzymatic hydrolysis to produce hydrolyzed cerebroprotein powder, take out the paste in the extraction kettle Ⅰ for the extraction of br...

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Abstract

The invention discloses a creative method for commercially producing hydrolyzed brain protein powder and cephalin by animal brains. The method comprises the steps of using supercritical carbon dioxide to remove grease, performing enzymolysis and using active carbon to decolorize; using macroporous resin to adsorb impurities, flowing out brain protein hydrolysate; using cation-anion resin to desalt; using the thin film to condensate the product, performing spray drying; obtaining hydrolysated brain protein powder and purifying the paste in a separation kettle I. The method overcomes the problem that the method for removing grease by the organic solvent produces residual solvent to cause the toxic reaction, removes grease cleanly, is easy to hydrolyze and filter, and strengthens fishy smell-removing and decolorizing ability of the active carbon due to macroporous resin; the creativity is as follows: the obtained hydrolysated brain protein powder is pink-white, has no odor and toxicity, and has high cephalin; two products can be produced at the same time, which can be applied in nutritious foods, health care products and foods, and can be manufactured drugs after being purified further.

Description

technical field [0001] The present invention relates to a kind of supercritical carbon dioxide extraction to remove lipids in animal brain tissue, the residual cerebroprotein in the composite enzyme hydrolysis extraction kettle is converted into amino acids and peptides, concentrated and dried to obtain cerebroprotein hydrolysis powder; the extract in the separation kettle Ⅰ is dissolved in acetone Cholesterol was removed, and cephalin was extracted by silica gel chromatography. The method belongs to the field of food industry. Background technique [0002] For a long time, people have used animal brain protein hydrolyzate to assist in the treatment of complications caused by Alzheimer's disease, stroke, traumatic brain injury, and cerebral thrombosis; infant brain dysplasia; dizziness caused by excessive use of the brain by white-collar workers have all received good results. . This is because the composition of animal brain tissue is similar to that of human brain tissue...

Claims

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Application Information

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IPC IPC(8): A23J1/00A23J3/34A23J3/04A23J7/00
Inventor 李世泰王清云林强
Owner LINGAO ZESHITAI BIOTECH CO LTD
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