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Preparation method of small molecule peptides of vinasse and application of small molecule peptides of vinasse in skin care products

A technology of small molecular peptides and distiller's grains, which is applied in the direction of cosmetic preparations, toiletry preparations, skin care preparations, etc., can solve the problems of low added value, achieve low cost, increase extraction rate, and simple preparation process.

Active Publication Date: 2018-05-04
GUANGDONG MARUBI BIOLOGICAL TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, it is mainly used in feed and food, with low added value

Method used

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  • Preparation method of small molecule peptides of vinasse and application of small molecule peptides of vinasse in skin care products
  • Preparation method of small molecule peptides of vinasse and application of small molecule peptides of vinasse in skin care products
  • Preparation method of small molecule peptides of vinasse and application of small molecule peptides of vinasse in skin care products

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] A preparation method of distiller's grain small molecule peptide, comprising the steps of:

[0037] (1) Extrude distiller's grains with a water content of 16% under the conditions of vacuum degree of -0.04MPa, temperature of 180°C, and screw speed of 150r / min, cooling, crushing, and sieving to obtain puffed distiller's grains samples;

[0038] (2) Weigh 5.00g distiller's grain puffed sample and pulverize it, extract it under the conditions of pH 11.0, liquid-to-solid ratio 20:1 (mL / mg), extraction time 150min, extraction temperature 50°C, and extract at pH 4.5 after the extraction Acid precipitation to obtain distiller's grain protein extract;

[0039] (3) Bromelain was used to hydrolyze distiller's grain protein. The enzymatic hydrolysis conditions were as follows: the amount of enzyme was 0.6% of the distiller's grain protein, the hydrolysis temperature was 50°C, the hydrolysis time was 120 min, and the pH was adjusted to the optimum value of bromelain with 0.1mol / L N...

Embodiment 2

[0041] A preparation method of distiller's grain small molecule peptide, comprising the steps of:

[0042] (1) Dried the distiller's grains to constant weight and crushed. The water content of the mixed material after adding water was 18%, and it was extruded under the conditions of vacuum degree of -0.01MPa, temperature of 200°C, and screw speed of 180r / min. Cooling, crushing, and sieving to obtain distiller's grain puffed samples;

[0043] All the other steps are the same as in Example 1.

Embodiment 3

[0045] A preparation method of distiller's grain small molecule peptide, comprising the steps of:

[0046] (1) Dried the distiller's grains to constant weight, crushed, and the water content of the mixed material after adding water was 20%, extruded and cooled under the conditions of a vacuum of 0.20MPa, a temperature of 200°C, and a screw speed of 220r / min , crushing, and sieving to obtain distiller's grain puffed samples;

[0047] All the other steps are the same as in Example 1.

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Abstract

The invention provides a preparation method of small molecule peptides of vinasse and application of small molecule peptides of vinasse in skin care products. The preparation method comprises the following steps: S1, carrying out vacuum extrusion, cooling, crushing and sieving on vinasse, the water content of which is 11-22%; S2, carrying out alkali dissolution and acid settlement on the vinasse expanded powder obtained in the S1 to extract vinasse proteins so as to obtain vinasse protein extract; and S3, carrying out hydrolysis on the vinasse protein extract extracted in the S2 with proteolytic enzymes, and carrying out enzyme deactivation, filtration, concentration, freezing and drying to obtain the small molecule peptides of vinasse. By deeply processing the vinasse by adopting a special vacuum expansion technology, the extraction ratio of the vinasse proteins is increased effectively; meanwhile, by adopting the special proteolytic enzymes, the small molecule peptides of vinasse with relatively good moisturizing and repairing functions can be obtained. In addition, the small molecule peptides of vinasse are applied to skin care products, so that the moisturizing and repairing functions of the skin care products are improved while the additional value of the vinasse is increased.

Description

technical field [0001] The invention belongs to the technical field of cosmetics preparation, and more specifically relates to a preparation method of distiller's grain small molecule peptide and the application of distiller's grain small molecule peptide in skin care products. Background technique [0002] At present, bioactive peptides are widely used in cosmetics, but the source is single, and animal-derived collagen peptides almost dominate the entire bioactive peptide skin care market. However, according to current literature and ancient prescription records, active peptides derived from plants also have good skin care effects. And existing studies have shown that oat protein peptides, soybean protein peptides, and peanut protein peptides have good moisturizing and antioxidant effects in vitro. However, there are too few plant-derived molecular peptides currently used in skin care products. [0003] Sake lees refers to the residue left after brewing Japanese sake. At...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P21/06A61K8/64A61K8/92A61Q19/00
CPCA61K8/645A61K8/922A61Q19/00C12P21/06
Inventor 刘露孙怀庆佐佐木公夫裴运林聂艳峰孙连立龙京洲
Owner GUANGDONG MARUBI BIOLOGICAL TECH CO LTD
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