The invention discloses a prediction algorithm for recognizing tyrosine posttranslational modification sites. The algorithm comprises the steps of data collection, data processing, feature coding, feature optimization and model training and evaluation. The invention furthermore discloses application of the prediction algorithm. According to the algorithm, features of the tyrosine posttranslational modification sites are extracted comprehensively from the perspectives of protein sequence information, evolutional information and physical and chemical properties, Elastic Net is used as an optimization means to automatically select variables to screen multidimensional features, redundant information is removed, a prediction model of tyrosine nitration, sulfuration and phosphorylation sites is constructed in combination with an SVM, the prediction capability of the prediction model is improved, and the prediction quality of the tyrosine posttranslational modification sites is remarkably improved. Through a developed prediction software platform TyrPred, predictive analysis of nitration modification sites, sulfuration modification sites and phosphorylation modification sites of tyrosine on intact protein is realized, and a convenient, economical and rapid research tool and important reference are provided for research of tyrosine posttranslational modification.