Trace detection method of vitamin A and vitamin E in peripheral blood
A detection method and vitamin technology, applied in the field of vitamin detection, can solve the problems of troublesome pretreatment steps, unoptimized improvement of detection time, low detection efficiency, etc., achieve simple, convenient and effective pretreatment steps, meet the needs of clinical detection, and have high efficiency Effect
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Embodiment 1
[0119] Experimental reagents: sample preparation solution, standard storage solution, instrument analysis solution.
[0120] The sample preparation solution includes:
[0121] Internal standard solution: prepared with methanol containing BHT1g / L d 4 -Internal standard solution of retinol, concentration 850ng / mL; d obtained with methanol configuration containing BHT1g / L 6 -Internal standard solution of α-tocopherol, concentration 10μg / mL;
[0122] tert-butyl methyl ether: volume 500mL;
[0123] Complex solution: 80% methanol;
[0124] Standard stock solution:
[0125] Vitamin A standard stock solution prepared with methanol containing BHT (1g / L), the concentration after dilution is 15, 100, 150, 200, 500, 1000, 1200, 1500, 2000ng / ml;
[0126] Vitamin E standard stock solution prepared with methanol containing BHT (1g / L), the concentration after dilution is 0.2, 0.5, 0.85, 1, 1.5, 5, 10, 12.5, 16, 20μg / mL;
[0127] Instrument analysis solution:
[0128] Mobile phase A: 0....
Embodiment 2
[0135] Embodiment two, the difference with embodiment one is:
[0136] Mass spectrometry conditions: electrospray ionization source (ESI), positive ion mode;
[0137] Spray capillary voltage (Capliary): 1.0KV;
[0138] Desolvation Temp: 650°C;
[0139] Desolvation gas flow rate (Desolvation): 800L / Hr;
[0140] Ion source temperature (Source Temp): 125°C;
[0141] In this example, the actual detection results did not change significantly.
Embodiment 3
[0142] Embodiment three, the difference with embodiment one is:
[0143] The injection volume was increased, but the actual test results did not change significantly.
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