Trace detection method of vitamin A and vitamin E in peripheral blood
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A detection method and vitamin technology, applied in the field of vitamin detection, can solve the problems of troublesome pretreatment steps, unoptimized improvement of detection time, low detection efficiency, etc., achieve simple, convenient and effective pretreatment steps, meet the needs of clinical detection, and have high efficiency Effect
Inactive Publication Date: 2018-11-13
杭州度安医学检验实验室有限公司
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[0010] 2. Although this technical solution can improve the specificity in the liquid chromatography tandem mass spectrometry detection step compared with the previous technology, its detection time has not been optimized and improved, resulting in low detection efficiency
[0011] 3. In the pretreatment step of this technical solution, it is necessary to add internal standard, methanol solution, zinc sulfate solution, n-hexane, nitrogen to dry and then add complex solution. The pretreatment steps are cumbersome, which makes the detection efficiency low
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[0121] Internal standard solution: prepared with methanol containing BHT1g / L d 4 -Internal standard solution of retinol, concentration 850ng / mL; d obtained with methanol configuration containing BHT1g / L 6 -Internal standard solution of α-tocopherol, concentration 10μg / mL;
[0122] tert-butyl methyl ether: volume 500mL;
[0123] Complex solution: 80% methanol;
[0124] Standard stock solution:
[0125] Vitamin A standard stock solution prepared with methanol containing BHT (1g / L), the concentration after dilution is 15, 100, 150, 200, 500, 1000, 1200, 1500, 2000ng / ml;
[0126] Vitamin E standard stock solution prepared with methanol containing BHT (1g / L), the concentration after dilution is 0.2, 0.5, 0.85, 1, 1.5, 5, 10, 12.5, 16, 20μg / mL;
[0127] Instrument analysis solution:
[0128] Mobile phase A: 0....
Embodiment 2
[0135] Embodiment two, the difference with embodiment one is:
[0136] Mass spectrometry conditions: electrospray ionization source (ESI), positive ion mode;
[0137] Spray capillary voltage (Capliary): 1.0KV;
[0138] Desolvation Temp: 650°C;
[0139] Desolvation gas flow rate (Desolvation): 800L / Hr;
[0140] Ion source temperature (Source Temp): 125°C;
[0141] In this example, the actual detection results did not change significantly.
Embodiment 3
[0142] Embodiment three, the difference with embodiment one is:
[0143] The injection volume was increased, but the actual test results did not change significantly.
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Abstract
The invention discloses a trace detection method of vitamin A and vitamin E in peripheral blood. The trace detection method is characterized by comprising the following steps: carrying out proprocessing on to-be-detected samples; taking peripheral blood samples and adding solutions containing internal standard; carrying out vortex oscillation to complete protein precipitation; adding tertiary butyl ether and carrying out vortex oscillation and extraction; centrifuging supernatant; blow-drying the supernatant by using nitrogen at a room temperature; adding a re-dissolution liquid; carrying outvortex oscillation and centrifugation to obtain the to-be-detected samples; carrying out ultrahigh performance liquid chromatography tandem mass spectrometry detection; obtaining the peak area of to-be-detected matter and the peak area of the corresponding internal standard in the to-be-detected samples through the ultrahigh performance liquid chromatography tandem mass spectrometry. According tothe trace detection method provided by the invention, high-speed high-efficiency detection on the vitamin A and the vitamin E in the trace peripheral blood samples can be carried out in 2 minutes.
Description
technical field [0001] The invention relates to the field of vitamin detection, in particular to a method for micro-detection of vitamin A and vitamin E in peripheral blood. Background technique [0002] Vitamins are a type of trace organic substances that humans and animals must obtain from food in order to maintain normal physiological functions. They play an important role in human growth, metabolism, and development. Although they do not participate in the formation of human cells, they also A class of organic compounds that do not provide energy to the human body, but are necessary to maintain good health. [0003] Vitamin A (Vitamin A), also known as retinol, is a class of fat-soluble vitamins. Its function is to maintain the normal visual function of animals, maintain the health of epithelial tissue cells, promote the synthesis of immunoglobulins, and promote growth and reproduction. Generally, it can be ingested from animal liver, dairy products or eggs, as well as ...
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