63results about How to "Meet the needs of clinical testing" patented technology

The invention relates to an enzyme-promoting chemiluminiscence substrate using alkaline phosphatase. Using water as solvent, the enzyme-promoting chemiluminiscence substrate comprises 2-amino-2-methyl-1-propyl alcohol, AMPPD, and a luminescence enhancer link-coupled by sodium fatty alcohol polyoxyethylene ether carboxylate and fluorescent compounds. According to the enzyme-promoting chemiluminiscence substrate using alkaline phosphatase, the luminescence enhancer can achieve the effect of co-surfactant, the compounds can be better combined into a chemiluminiscence buffer system, and thus the chemiluminiscence efficiency is greatly improved. The enzyme-promoting chemiluminiscence substrate using alkaline phosphatase has the advantages of being high in strength, high in sensitivity, long in duration, good in stability and the like. Clinical testing requirements can be met completely, the main performance index has already reached the level of foreign products, and the cost of the chemiluminiscence substrate is greatly lowered.

The invention relates to the technical field of detection of ischemia modified albumin content, and in particular to a detection kit for ischemia modified albumin. The kit includes a reagent 1 and a reagent 2. The reagent 1 contains a buffer, cobalt chloride, a stabilizer and a preservative; and the reagent 2 contains a buffer, dithiothreitol, a stabilizer, a reducing protective agent and a preservative. The kit can accelerate the binding efficiency of cobalt ions with normal albumin, and the cobalt ions fully integrate with normal albumin in a certain period of time, so as to ensure the accuracy of the detection result on a sample by the reagent; the kit protects the stability of dithiothreitol in the solution, and does not affect the binding of dithiothreitol with cobalt ions, so as to ensure that an open reagent bottle can be stably kept in a dark place for 30 days at room temperature and a closed reagent bottle can be stably kept at 2-8 DEG C for 12 months, and fully meet the needs of clinical laboratory; and the kit accelerates the combination of dithiothreitol with cobalt ions, so that the reagents reach reaction endpoints as soon as possible, thereby ensuring the efficient detection effect of reagents and significantly improving the accuracy of reagent detection.

The invention relates to a high-sensitivity quantitative determination method of cardiac troponin I, and belongs to the technical field of biomedical detection. According to the detection method, specific recognition is conducted on the cardiac troponin I through an aptamer, and accurate and sensitive quantitative detection on cTnI is achieved by means of a rolling circle amplification technology of nucleic acid and a graphene oxide-based fluorescence resonance energy transfer technology; the detection method has the advantages of being good in specificity, high in sensitivity, easy and convenient to operate, low in cost and the like, and the clinical test requirements are met.

The invention discloses long-optical-path enzyme-linked immunoassay for testing thyroid stimulating hormone and provides a kit of the method. The long-optical-path enzyme-linked immunoassay comprises the following test steps of: performing immune reaction, separating, developing, measuring and processing data. A long-optical-path flowing sample pool and an optical fiber spectrometer are adopted for measurement; the detection sensitivity is 0.015mIU / L by increasing a measuring optical path, adopting a low-background substrate solution as well as optimizing a component preparation method; and compared with the sensitivity of the conventional optimized enzyme-linked immunoassay in which the same antibody is used, the sensitivity is enhanced by four times. The test linear range is 0.05 to 20mIU / L. The immune reaction is performed in a wrapped tube, and the reaction mode is a dual antibody sandwich one-step method. The kit comprises an antibody coated tube, a serial calibrator, an enzyme marker, a condensed cleaning solution, the low-background substrate solution, a terminating solution and an end-product diluent. The method is high in sensitivity and proper in test range, and the requirement of clinical detection can be met; meanwhile, the kit has low manufacturing cost and is favorable to popularization and application.

The invention discloses an recombinant antigen and a rapid gold-marking diagnosis paper strip for 1-type dengue. The invention mainly aims to provide an outer-membrane protein DIII region recombination antigen for the 1-type dengue diagnosis and can simultaneously aims to a rapid gold-marking diagnosis test paper strip used for detecting IgM, IgG antibodies in sera of 1-type dengue virus infected persons. The test paper strip utilizes a gene clone technology to obtain the outer-membrane protein DIII region recombination antigen of a 1-type dengue virus, and the outer-membrane protein DIII region recombination antigen can be used for immunology diagnosis after purification; the rapid gold-marking diagnosis test paper strip can simultaneously be used for detecting the IgM, IgG antibodies in the sera of the 1-type denguen virus infected persons, so as to judge the infection states of patients, to do early diagnosis on the patients, so that the important action for controlling an infection source and reducing disease spread; in addition, according to the gold-marking diagnosis test paper strip, a specific instrument is not required, and well-trained professional staff is not required, so that requirements of clinic detection in substrate hospitals and rural health centers can be met; and the cost is greatly reduced, the convenience is brought to the patients, and the test paper strip is suitable for large-scale seroepidemiological survey.

The invention provides a glutathione reductase assay kit, which comprises a reagent R1 and a reagent R2. The reagent R1 includes: 50-200 mmol / L of a Tris buffer solution, 0.5-2 mmol / L of EDTA, 1-5 kU / L of pyruvic carboxylase, 1-5 kU / L of ascorbic acid oxidase, 5-20 mg / L of potassium ferrocyanide, a surfactant and a preservative; the reagent R2 includes: 50-200 mmol / L of the Tris buffer solution, 0.5-2 mmol / L of EDTA, 2-10 mmol / L of GSSG, 0.1-0.5 mmol / L of NADPH, 1-15 g / L of a stabilizer, and 0.5-2 g / L of a preservative. The liquid assay kit has good stability and is strong in anti-interferenceeffect. The invention also discloses a preparation method and an application of the assay kit.

The invention discloses a dry type biochemical analyzer belonging to the technical field of biochemical detection equipment. The dry type biochemical analyzer is characterized in that in a mechanical system, a dry sheet sampling position is arranged on an X and Y two-dimensional linear movement platform, two pushing rods are respectively arranged in an X direction of the X and Y two-dimensional linear movement platform, an incubation groove is fixed in front of the pushing rods, a baffle block is arranged in the front end of the incubation groove, and a pushing rod is arranged in a Y direction of the X and Y two-dimensional linear movement platform. In an optical system, (a), an LED (Light Emitting Diode) light source is arranged in an integrating sphere, a lens groove is arranged on an optical axis line of the integrating sphere and a dry sheet; and (b), a rear beam-splitting optical system is located at the underface position vertical to the dry sheet, and a lens group and a grating are arranged on a lower vertical line of the dry sheet. The dry type biochemical analyzer has the beneficial effects that 1, by applying an integrating sphere technology, the problem of difficulty in acquiring a signal due to diffuse reflection is solved, and the precision of an instrument is greatly improved; 2, the instrument is more flexible and is convenient to use; 3, by adopting a rear beam-splitting design, the detection accuracy is improved, and the stability of unit is higher; and 4, the dry type biochemical analyzer is low in cost, small and portable, and high in detection speed and performs detection for 120 tests per hour by applying a multi-channel means; and multiple reagent dry sheets are matched with the instrument, thus the demand of clinical testing is met.

The present invention relates to an alkaline phosphatase enzymatic chemiluminescent substrate. The enzymatic chemiluminescent substrate uses water as a solvent, and also comprises the following components: 2-amino-2-methyl-1-propanol; AMPPD; a luminescence enhancer which is sodium fatty alcohol polyoxyethylene ether carboxylate coupled with a fluorescent compound. The luminescence enhancer has a co-surfactant effect, allowing the complex to be better combined into a chemiluminescent buffer system, thereby significantly increasing the chemiluminescent efficiency. The alkaline phosphatase enzymatic chemiluminescent substrate has advantages such as high strength, high sensitivity, a long duration and good stability, and fully satisfies clinical detection requirements. A main performance indicator of the chemiluminescent substrate reaches a foreign product level, and the costs of the chemiluminescent substrate are significantly reduced.

The invention discloses a circulating tumor cell capture system. The circulating tumor cell capture system includes a supporting device, a plugging device, a filtering device, a scanning device, a pipetting device and a suction filtering device. The support device has a support seat and a base plate, and the base plate is slidably connected to the support seat; the plug removal device is used to remove the plug on the sample tube; the filter device has a filter; the scanning device It is used to scan and record the information marking code of the sample tube and the information marking code of the filter; the pipetting device is used to suck the sample liquid from the sample tube and send it to the filter; the suction filtering device has a suction head and Suction filter, the suction filter suction head is arranged on the support base for the connection of the filter, the suction filter is communicated with the suction filter suction head for the filter suction head Suction filter described above. The circulating tumor cell capture system has the advantages of simple operation, good stability and high sensitivity.

The invention discloses a protein C activity determination kit based on a chromophoric substrate method, protein C is a vitamin K dependent plasma serine protease zymogen, and comprises a reagent R1, a reagent R2 and a diluting reagent; the R1 reagent comprises a protein C activator, a first buffer solution and a first auxiliary material; the R2 reagent comprises a chromogenic substrate Pca-5297, a second buffer solution and a second auxiliary material; the diluting reagent comprises a third buffer solution and a third auxiliary material, and the pH of the first buffer solution, the pH of the second buffer solution and the pH of the third buffer solution are 7.2-7.6; the protein C activator and the chromophoric substrate Pca-5297 are matched for use, during detection, the cutting efficiency is high, the reaction signal is strong, the reaction speed is high, the sensitivity is high, the linear range is wide, the reaction time is short, the sample distinction degree is increased, and establishment of the linear range and clinical sample testing are facilitated; the reagent is liquid, convenient to use, low in cost and good in stability.