Methyl esterification luminol enzyme-catalyzed chemical luminescence substrate system

Abstract

The invention discloses a methyl esterification luminol enzyme-catalyzed chemical luminescence substrate system, which consists of a reagent A and a reagent B, wherein the reagent A consists of 0.90 mM of methyl esterification N-(4-amino butyryl)-N-ethyl-iso-luminol and 0.01 to 0.05 M of a carbonate buffer solution with the pH value of 9.6; and the reagent B consists of 0.01 to 0.05 M of a carbonate buffer solution of with the pH value of 9.6, 0.05 to 0.1 mM of urea peroxide and 0.15 to 0.30 mM of peroxy phenol. In 4 to 6 magnitudes, the luminous intensity of the methyl esterification luminol enzyme-catalyzed chemical luminescence substrate system shows a good linear relation with the concentration of measuring substances, and in a wider testing range, the methyl esterification luminol enzyme-catalyzed chemical luminescence substrate system can ensure high sensitivity and completely meet requirements on a clinical test.

Description

technical field [0001] The invention relates to an enzymatic chemiluminescent substrate, in particular to a methylated luminol enzymatic chemiluminescent substrate system. Background of the invention [0002] In recent years, with the progress of molecular biology, basic immunology and immunochemistry and the development of instrumental analysis using modern high-tech, immune labeling technology has also been continuously improved and updated; radioimmunoassay and enzyme-linked immunosorbent technology have been gradually Luminous technology is superseded. Today, enzymatic substrate luminescence technology has become the fastest popularized and applied immunoassay method. Enzymatic substrate luminescence technology is to replace the chromogenic substrate in the enzyme-linked immunoassay method with a luminescent substrate, which undergoes a redox reaction with the corresponding enzyme to generate light quanta; the amount of the substance to be detected is determined by coun...

AI Technical Summary

Problems solved by technology

Horseradish peroxidase (HRP), the preferred marker for enzymatic luminescence, uses luminol as a luminescent substrate, but it is necessary to add a variety of enhancers (solutions) and catalysts. Composition to solve the problems of weak light signal, low sensitivity and short range. After reviewing domestic and foreign literature, it is found that most researchers are improving the enhancer to improve the technical level, and few of the luminescent substrate systems, especially Conduct research on substrate systems that do not require enhancers

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