Cyanogens-free hemolytic agent for hemocyte analyzer

Abstract

The invention relates to a cyanogens-free hemolytic agent for hemocyte analyzer. One litre of the cyanogens-free hemolytic agent comprises the following components: 0.8-5.0 g of potassium chloride, 0-60.0 g of dodecyl trimethyl ammonium chloride, 0-14.0 g of octadecyl trimethyl ammonium bromide, 6.0-10.0 ml of isopropanol, a carbonate or hydrochloric acid buffer solution, and the balance of water, wherein the carbonate or hydrochloric acid buffer solution is used for regulating pH to 3.2-5.8; the addition quantities of the dodecyl trimethyl ammonium chloride and the octadecyl trimethyl ammonium bromide are complementary, but the sum of the addition quantities of the dodecyl trimethyl ammonium chloride and the octadecyl trimethyl ammonium bromide is less than or equal to 60 g. The invention can form stable hemoglobin derivatives and meet the requirements of clinical examination because absorption spectrum curves are similar when lambda is equal to 540 nanometers and lambda is equal to 504 nanometers; and in addition, the invention can effectively improve the work environments of operators and reduce the injury of poisons on human body health because reagents do not contain cyanides and trinitrides or have no toxicity.

Description

technical field [0001] The invention belongs to the field of reagents for medical diagnostic instruments, and particularly relates to reagents for blood cell analyzers. Background technique [0002] Hemolytic agents are the most commonly used reagents in blood analyzers in medical tests, and are key reagents for the determination of hemoglobin (Hb) and white blood cell (WBC) counts or classifications. The hemolytic agent, under the coordination of the diluent, on the one hand, rapidly dissolves the red blood cells (RBC), releases the hemoglobin derivative and combines with the hemolytic agent to form a stable hemoglobin for quantitative determination of Hb, on the other hand, the leukocytes maintain their original form. And keep a certain volume for WBC count, and according to the size of the white blood cells to three groups of white blood cells (lymphocytes, neutrophils, intermediate cells). In 1996, the International Committee for Standardization of Hematology recommende...

AI Technical Summary

Problems solved by technology

However, the biggest disadvantage of this compatible hemolytic agent is that KCN is highly toxic, which not only seriously endangers the health of laboratory workers, but also brings a lot of inconvenience to the operation of the production unit and seriously pollutes the environment.

Although extremely strict and cumbersome measures to reduce cyanide pollution have been formulated clinically, its pollution to the human body and the environment is still unavoidable.

[0004] A cyanide-free hemolytic agent is disclosed in Chinese patent CN99115572.6, but it can only be used in CD series and F series blood cell analysis instruments, and cannot be used in other series of instruments

The product is harmful to the human body when inhaled, and there are some problems in its promotion to practical application, and its application range is also limited

It has not fundamentally solved the problem that blood test personnel, including production personnel, are harmed by toxins, such as cyanide pollution, and contamination of operators by cetyl powder in preparation

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