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Preserving fluid as well as preparation method and application thereof

A technology of preservation solution and protective agent, applied in the field of biomedicine, can solve the problems of poor circulating tumor cell activity, red blood cell or platelet aggregation, limited clinical use, etc., and achieve the effect of good cell activity and reducing aggregation.

Active Publication Date: 2019-03-22
GUANGZHOU IMPROVE MEDICAL TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Currently, EDTA anticoagulant tubes are the most frequently used preservation solution, but in the long-term preservation of circulating tumor cells, the activity of circulating tumor cells is poor, and the aggregation of red blood cells or platelets is prone to occur, which greatly limits the clinical use.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Weigh 2g of platelet aggregation inhibitor, 15g of cell membrane protective agent, 10g of pH regulator and 10g of anticoagulant and mix them, add 95ml of water and stir evenly to obtain the first product 1, wherein the platelet aggregation inhibitor is acetylsalicylic acid, cell membrane protection The agent is vitamin E, the pH regulator is citric acid and sodium citrate, and the anticoagulant is disodium edetate. The first product 1 was mixed with 0.02 g of the osmotic protectant, added water to make up to 100 ml, stirred evenly, and filtered through a filter membrane with a pore size of 0.22 um to obtain a preservation solution, wherein the osmotic protectant was glycerin.

Embodiment 2

[0032] Weigh platelet aggregation inhibitor 2.5g, cell membrane protective agent 6g, pH regulator 4g and anticoagulant 12g after mixing, add 85ml water and stir evenly, obtain the first product 2; Wherein the platelet aggregation inhibitor is ticlopidine, cell membrane The protective agent is sucrose, the pH regulator is phosphate, and the anticoagulant is disodium edetate. The first product 2 was mixed with 0.2 g of the osmotic protectant, added water to make up to 100 ml, stirred evenly, and filtered through a filter membrane with a pore size of 0.22 um to obtain a preservation solution, wherein the osmotic protectant was glycerin.

Embodiment 3

[0034] Weigh platelet aggregation inhibitor 5g, cell membrane protective agent 1.5g, pH regulator 3g and anticoagulant 20g after mixing, add 85ml water and stir evenly, obtain the first product 3; Wherein the platelet aggregation inhibitor is ticlopidine, cell membrane The protective agent is polyvinylpyrrolidone, the pH regulator is potassium hydroxide, and the anticoagulant is tripotassium edetate. Mix the first product 3 with 0.1 g of the osmotic protectant, add water to make up to 100 ml, stir evenly, and filter with a filter membrane with a pore size of 0.22 μm to obtain a preservation solution, wherein the osmotic protectant is DMSO.

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PUM

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Abstract

The invention relates to the technical field of biomedicines, in particular to a preserving fluid as well as a preparation method and application thereof. The invention discloses the preserving fluidwhich comprises the following raw materials: a platelet aggregation inhibitor, a cell membrane protector, a permeability protector, a pH regulator and an anticoagulation agent. The preserving fluid iscapable of maintaining activity of circulating tumor cells and reducing erythrocyte or platelet aggregation.

Description

technical field [0001] The invention relates to the field of biomedical technology, in particular to a preservation solution and its preparation method and application. Background technique [0002] Since Australian pathologist John Ashworth first proposed the concept of circulating tumor cells in 1869, with the development of research on circulating tumor cells, it has been discovered that the detection of circulating tumor cells can monitor tumor dynamics in real time, individualize treatment, evaluate treatment effects and It plays an important role in guiding the prognosis of patients. Compared with traditional imaging diagnosis, endoscopy and pathological diagnosis, the detection of circulating tumor cells has significant advantages. It can more sensitively detect the changes of the disease, and evaluate the effect of the treatment plan more scientifically and quickly. However, traditional blood collection tubes cannot preserve the activity of circulating tumor cells f...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N1/02
CPCA01N1/0226
Inventor 李思韵张姝钟经德王思娜
Owner GUANGZHOU IMPROVE MEDICAL TECH CO LTD
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