The invention relates to a
fluorescence and colorimetric dual-mode biosensing method for detecting
caffeine and application thereof, the method causes
CRISPR / Cas13a to regulate and control Fe-MOFs to fall off from a
signal probe based on to-be-detected liquids with different concentration gradients, the fallen Fe-MOFs collapses under the action of
sodium pyrophosphate to release 2-aminoterephthalic acid and
ferric ions, and the 2-aminoterephthalic acid and
ferric ions are subjected to
fluorescence and colorimetric dual-mode biosensing method is used for detecting
caffeine. 2-aminoterephthalic acid is converted into a
fluorescence signal, meanwhile,
Prussian blue generated by reaction of
ferric ions and
potassium ferrocyanide is converted into a colorimetric
signal, and a fluorescence and colorimetric dual-mode biosensing method is constructed; according to the sensor, interference factors can be effectively eliminated by outputting two detection signals and performing
cross validation; the advantages of high sensitivity and wide
linear range of a fluorescence method and the advantages of simple operation and visual result of a colorimetric method are combined, so that a target object can be accurately recognized, the lower
detection limit can be reduced, and the accuracy of a
quantitative result can be ensured; according to the sensing method,
visual observation can be achieved, precise instruments are not needed, and good application value is achieved.