Method for calculating contents of three effective components in curcuma longa product through relative correction factor

A technology of relative correction factor and active ingredient, applied in the direction of measurement device, material separation, analysis of materials, etc., can solve the problem of single curcumin index, difficult to comprehensively reflect the quality and efficacy, etc., to provide work efficiency, easy operation, The effect of reducing waste

Inactive Publication Date: 2015-12-02
SICHUAN NEO GREEN PHARMA TECH DEV
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Problems solved by technology

At present, curcumin is used as a single indicator for the determination of the content of turmeric med

Method used

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  • Method for calculating contents of three effective components in curcuma longa product through relative correction factor
  • Method for calculating contents of three effective components in curcuma longa product through relative correction factor
  • Method for calculating contents of three effective components in curcuma longa product through relative correction factor

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0049] According to the steps of the present invention, carry out the calculation of three kinds of active ingredient contents in the calculation of the preparation of solution, relative correction factor turmeric product, concrete process is as follows:

[0050] 1. Preparation of reference substance stock solution

[0051] Accurately weigh curcumin, demethoxycurcumin and bisdemethoxycurcumin, mix them, and add methanol solution to prepare mixed reference substance stock solutions with concentrations of 98.88, 44.40, and 25.92 μg / mL, respectively.

[0052] 2. Preparation of reference solution

[0053] Precisely measure 0.3mL, 0.6mL, 1.2mL, 2.5mL of the above-mentioned stock solutions, and dilute them to 5mL with methanol solution respectively to obtain mixed solutions of reference substances with different concentrations.

[0054] 3. Preparation of the test solution

[0055] Take 0.2g of the sample to be tested, add 25mL of methanol to dissolve, weigh the mass, ultrasonicall...

Embodiment 2

[0108] In order to test the reliability and accuracy of the above-mentioned relative correction factor f, the following testing instruments, chromatographic columns, detection wavelength, column temperature, flow rate, flow relative ratio and other tests were designed to verify the relative correction factor f under different test conditions The RSD value of each experiment is used to determine the stability and accuracy of f. But it should be stated that the following experiments do not affect the test method and results of the present invention.

[0109] 1. The influence of the detection instrument on the relative correction factor f

[0110] Under the same chromatographic conditions, the influence of Agilent, Shimadzu, and Waters three types of chromatographs on f was investigated, and the detection results and calculation results of relative correction factors are shown in Table 11 below:

[0111] Table 11 Test results and relative correction factor calculation results un...

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Abstract

The invention relates to a method for calculating the contents of three effective components in a curcuma longa product through a relative correction factor, and belongs to the technical field of drug quality determination. The method comprises: (1) preparing a reference substance stock solution; (2) preparing a reference substance solution; (3) preparing a sample solution; (4) determining through high performance liquid chromatography; (5) determining the relative correction factor value; (6) calculating the curcumin content; and (7) calculating the demethoxycurcumin content and the bisdemethoxycurcumin content. According to the present invention, the relative correction factor is used to detect the contents of the three effective components in the curcuma longa product, such that the demethoxycurcumin reference substance preparation and the bisdemethoxycurcumin reference substance preparation in each experiment are eliminated, the detection cost is reduced, and the detection efficiency is improved; the relative correction factor f is verified under different detection equipment, different chromatographic columns, different detection wavelengths, different column temperatures, different flow rates and different flow relative ratios; and the method has characteristics of rapidness, efficiency, high precision, low cost and the like, and is the feasible and effective detection method.

Description

technical field [0001] The invention belongs to the technical field of drug quality determination methods, and specifically relates to a method for calculating the contents of three active ingredients in turmeric products with relative correction factors. Background technique [0002] Traditional Chinese medicine has the characteristics of multi-component, multi-target, multi-level, and overall effect. However, at present, the content of a single index component in the medicinal material is often used as the evaluation index for its quality control, which is difficult to accurately and comprehensively reflect its quality, let alone comprehensively reflect its functions and effects. Turmeric is the dry rhizome of Curcumalonga L., a plant of the Zingiberaceae family. It is a traditional medicinal material for both medicine and food. Lump in the abdomen, rheumatic shoulder and arm pain, tumbling and swelling pain, etc. At present, curcumin is used as a single indicator for th...

Claims

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Application Information

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IPC IPC(8): G01N30/02
Inventor 周厚成胡昌江李文兵吴文辉周维冯健
Owner SICHUAN NEO GREEN PHARMA TECH DEV
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