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Steady ischemia modified albumin kit

An albumin and kit technology, applied in the field of ischemia-modified albumin kits, can solve the problems of short half-life solution, poor stability, oxidation, etc., and achieve the effects of improving stability and stable test performance.

Active Publication Date: 2014-12-10
NINGBO RUI BIO TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, since DTT itself is a strong reducing agent, it is easily oxidized by air, and its half-life is relatively short, so its stability is very poor.

Method used

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  • Steady ischemia modified albumin kit
  • Steady ischemia modified albumin kit
  • Steady ischemia modified albumin kit

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] The stable ischemia-modified albumin kit in this example is composed of cobalt ion reagent and dithiothreitol reagent;

[0034] Among them, the components and concentrations of the cobalt ion reagent are: triethanolamine buffer solution: 100mmol / L, cobalt chloride: 0.2g / L, TritonX-100: 1g / L, sodium azide: 0.5g / L, wherein, The pH value of triethanolamine buffer solution is 8.0;

[0035] The components and concentrations of dithiothreitol reagent are: 2-morpholineethanesulfonic acid buffer: 100mmol / L, DTT: 1.0g / L, TritonX-100: 1g / L, mannitol: 5-20g / L L, sodium azide: 0.5g / L, wherein, the pH value of the 2-morpholineethanesulfonic acid buffer is 5.5.

Embodiment 2

[0037] The stable ischemia-modified albumin kit in this example is composed of cobalt ion reagent and dithiothreitol reagent;

[0038] Among them, the components and concentrations of the cobalt ion reagent are: triethanolamine buffer solution: 100mmol / L, cobalt chloride: 0.2g / L, TritonX-100: 1g / L, sodium azide: 0.5g / L, wherein, The pH value of triethanolamine buffer solution is 8.0;

[0039] The components and concentrations of dithiothreitol reagent are: 2-morpholineethanesulfonic acid buffer: 100mmol / L, DTT: 1.0g / L, TritonX-100: 1g / L, glucose: 10g / L, Sodium nitrogen: 0.5g / L, wherein the pH value of the 2-morpholineethanesulfonic acid buffer is 5.5.

Embodiment 3

[0041] The stable ischemia-modified albumin kit in this example is composed of cobalt ion reagent and dithiothreitol reagent;

[0042] Among them, the components and concentrations of the cobalt ion reagent are: triethanolamine buffer solution: 100mmol / L, cobalt chloride: 0.2g / L, TritonX-100: 1g / L, sodium azide: 0.5g / L, wherein, The pH value of triethanolamine buffer solution is 8.0;

[0043] The components and concentrations of dithiothreitol reagent are: 2-morpholineethanesulfonic acid buffer: 100mmol / L, DTT: 1.0g / L, TritonX-100: 1g / L, glycerol: 10g / L, Sodium nitrogen: 0.5g / L, wherein the pH value of the 2-morpholineethanesulfonic acid buffer is 5.5.

[0044] figure 1 It is the calibration curve of the calibrator with a concentration of 70.6U / ml for the stable ischemia-modified albumin kit of the present invention, figure 1 The reading points in are every 34 points for 10 minutes. from figure 1It can be seen that the kit of the present invention can quickly reach the en...

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Abstract

The invention relates to a steady ischemia modified albumin kit, which comprises a cobalt ion reagent and a dithiothreitol reagent, wherein the cobalt ion reagent comprises the following components: 50-500 mmol / L of buffer solution, 0.01-1.0 g / L of cobalt chloride, 1 g / L of surface active agent, and 0.5 g / L of preservative; and the dithiothreitol reagent comprises the following components: 50-300 mmol / L of buffer solution, 0.01-1.0 g / L of dithiothreitol (DTT), 1 g / L of surface active agent, 1-20 g / L of protective agent, and 0.5 g / L of preservative. The kit disclosed by the invention can reach reaction endpoint rapidly and is steady in property; after being opened, the kit can be stably stored in a dark place at room temperature for 30 days; after being closed, the kit can be stably stored at 2 DEG C -8 DEG C for 12 months, and therefore, clinical test requirements can be completely satisfied.

Description

technical field [0001] The invention relates to the technical field of ischemic modified albumin detection, in particular to a stable ischemic modified albumin kit. Background technique [0002] Ischemia-modified albumin (IMA) is a new and ideal marker of ischemia. When ischemia or reperfusion occurs, the amino acid sequence of serum albumin is destroyed by free radicals, resulting in albumin and The ability to bind transition metals is altered, thereby producing albumin with an altered ability to bind transition metals. [0003] As an index to detect early myocardial ischemia, IMA can sensitively reflect myocardial ischemia, so it is of great significance in the early diagnosis, risk stratification, and guidance of treatment of acute coronary syndrome. At present, the IMA detection kit commonly used at home and abroad is composed of liquid double reagents, the first reagent is cobalt ion reagent, and the second reagent is dithiothreitol (DTT) reagent. However, since DTT i...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/68G01N21/78G01N21/31
CPCG01N21/31G01N21/78G01N33/6893G01N2800/325
Inventor 张丽张闻周海滨王建飞
Owner NINGBO RUI BIO TECH
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