Stable ischemia modified albumin testing kit

A technology of albumin and kit, which is applied in the field of stable ischemia-modified albumin assay kit, which can solve the problems of poor stability of DTT, easy oxidative decomposition of DTT, and easy oxidation by air, so as to achieve good stability and improve stability , the effect of slowing down the rate of oxidation

Active Publication Date: 2011-08-10
SICHUAN XINCHENG BIOLOGICAL CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, since DTT itself is a strong reducing agent, it is easily oxidized by air, so the stability of DTT is poor.
At present, the ACB? Test kit sold abroad, because DTT is easy to oxidize and decompose, the validity period is only 14 days

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] The invention comprises cobalt ion reagent and dithiothreitol reagent, and the volume mixing ratio thereof is: cobalt ion reagent, 50%; dithiothreitol reagent, 50%.

[0028] Reagent 1: cobalt ion reagent (by weight percentage), its component composition is:

[0029] Cobalt chloride hexahydrate 0.001%

[0030] Sodium azide 0.01%

[0031] Tris buffer 0.5%

[0032] Purified water 99.489%

[0033] The cobalt ion reagent is obtained after mixing the above components.

[0034] Reagent 2: dithiothreitol reagent (by weight percentage), its composition is:

[0035] Dithiothreitol 0.05%

[0036] Sodium azide 0.01%

[0037] Triton 0.01%

[0038] Imidazole buffer solution 0.5%

[0039] Purified water 99.43%

[0040] After mixing the above components, the dithiothreitol reagent is obtained.

[0041] Store the kit with the above components at 2-8°C, and measure the purified water after 6 months (at this time, the absorbance is the largest and stable for observation). Under...

Embodiment 2

[0046] The present invention comprises cobalt ion reagent and dithiothreitol reagent, and its volume mixing ratio is: cobalt ion reagent, 65%; dithiothreitol reagent, 35%.

[0047] Cobalt ion reagent (by weight percentage), its component composition is:

[0048] Cobalt chloride hexahydrate 0.05%

[0049] Sodium azide 0.51%

[0050] Tris Buffer 5%

[0051] Purified water 94.44%

[0052] The cobalt ion reagent is obtained after mixing the above components.

[0053] Reagent 2, dithiothreitol reagent (by weight percentage), its composition is:

[0054] Dithiothreitol 0.51%

[0055] Sodium azide 0.51%

[0056] Polyoxyethylene alkylphenol ether 0.51%

[0057] Imidazole buffer solution 5%

[0058] Purified water 93.47%

[0059] Dithiothreitol reagent is obtained after mixing the above ingredients.

[0060] The kit with the above components was stored at 2-8°C for 6 months and then tested for purified water. Under the condition of 37°C, use a spectrophotometer to continuous...

Embodiment 3

[0065] The present invention includes reagent 1 and reagent 2, and their volume mixing ratios are: reagent 1, 80%; reagent 2, 20%.

[0066] Reagent 1: cobalt ion reagent (by weight percentage), its component composition is:

[0067] Cobalt chloride hexahydrate 0.1%

[0068] Sodium azide 1%

[0069] Tris Buffer 10%

[0070] Purified water 88.9%

[0071] The cobalt ion reagent is obtained after mixing the above components.

[0072] Reagent 2, dithiothreitol reagent (by weight percentage), its composition is:

[0073] Dithiothreitol 1%

[0074] Sodium azide 1%

[0075] Sodium dodecylbenzenesulfonate 1%

[0076] Imidazole buffer solution 10%

[0077] Purified water 87%

[0078] Dithiothreitol reagent is obtained after mixing the above ingredients.

[0079] The kit with the above components was stored at 2-8°C for 6 months and then tested for purified water. Under the condition of 37°C, use a spectrophotometer to continuously measure the absorption of the reaction soluti...

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PUM

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Abstract

The invention discloses a stable ischemia modified albumin testing kit which comprises a cobalt ion reagent and a dithiothreitol (DTT) reagent. A small amount of nonionic surfactant is added in the dithiothreitol reagent, thus the combination of DTT in the reagent and free oxygen is greatly reduced, the oxidation speed of DTT is reduced, and the stability of the reagent is remarkably improved. Simultaneously, the concentration of DTT is increased, thus the stability of the kit is improved to a certain extent. The stable ischemia modified albumin testing kit disclosed by the invention can be stored stably in an opened bottle in a dark place at the room temperature for 30 days, and also can be stored stably in a closed bottle at the temperature of 2 to 8 DEG C for 12 months, and can totally meet the requirements for clinical examinations.

Description

technical field [0001] The invention relates to the field of biochemical reagents, in particular to a stable ischemia-modified albumin assay kit. Background technique [0002] Ischemia-modified albumin (IMA) is when the body undergoes ischemia or reperfusion, due to the destruction of the amino acid sequence of serum albumin by free radicals, resulting in changes in the binding ability of albumin and transition metals, while Produced albumin with altered ability to bind transition metals. [0003] In 1990, Bar. Or et al first applied the albumin cobalt binding test (albumin cobalt binding test, ACB) to measure the concentration of plasma IMA. That is, the sample and the cobalt chloride (CoCl 2 ) solution mixed, Co 2 + Binding to albumin with normal binding ability, after adding the second reagent, free Co 2 + Combining with dithiothreitol (DTT) produces a red reaction, and the absorbance is detected at 505nm. When the body is ischemic, albumin in the plasma is mostly...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/68G01N21/31
Inventor 高命陈卫周帅谭韬
Owner SICHUAN XINCHENG BIOLOGICAL CO LTD
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