Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Glutathione peroxidase determination kit, preparation method of glutathione peroxidase determination kit and application of glutathione peroxidase determination kit

A technology of glutathione peroxide and glutathione, which is applied in the direction of biological testing, material inspection products, etc., can solve the problems of cumbersome steps, high price, waste, etc., and achieve improved sensitivity, improved stability, improved The effect of reactivity

Active Publication Date: 2019-01-18
中拓生物有限公司
View PDF1 Cites 4 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, the main method for the determination of glutathione peroxidase in China is the Elisa method, which requires manual operation, and the steps are cumbersome, time-consuming, and expensive.
At present, the reagents that can be used in automatic biochemical analysis in China are ultraviolet enzymatic glutathione peroxidase kits, which are imported dry powder reagents. Although the results are accurate, they are expensive and require reconstitution before use, which is inconvenient to operate.
It needs to be used as soon as possible after reconstitution, it is very inconvenient to use and there will be a lot of waste if it is not used up after reconstitution

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Glutathione peroxidase determination kit, preparation method of glutathione peroxidase determination kit and application of glutathione peroxidase determination kit
  • Glutathione peroxidase determination kit, preparation method of glutathione peroxidase determination kit and application of glutathione peroxidase determination kit
  • Glutathione peroxidase determination kit, preparation method of glutathione peroxidase determination kit and application of glutathione peroxidase determination kit

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] The components of reagent R1 are: Tris buffer 100mmol / L, EDTA 0.5mmol / L, glutathione reductase 1KU / L, reduced glutathione 5mmol / L, potassium chloride 6g / L, magnesium chloride 0.3g / L, Tween 20 1ml / L, tyrosine 1g / L, sodium azide 0.5g / L;

[0040] The components in reagent R2 are: Tris buffer 100mmol / L, EDTA 0.5mmol / L, NADPH 1mmol / L, cumene hydroperoxide 0.3mmol / L, triton 100 1ml / L, sucrose 5g / L , Sodium azide 0.5g / L.

[0041] The pH value of reagent R1 is 7.0; the pH value of reagent R2 is 9.5;

[0042] Preparation method: (1) Preparation of reagent R1: take an appropriate amount of water, add the raw materials shown in R1 respectively, stir well to dissolve one raw material, then add the next raw material, adjust to the required pH value with hydrochloric acid or sodium hydroxide, and constant volume to required volume. (2) Preparation of reagent R2: Take an appropriate amount of water, add the raw materials shown in R1 respectively, stir well to dissolve one raw mate...

Embodiment 2

[0044] The components of reagent R1 are: Tris buffer 80mmol / L, EDTA 0.8mmol / L, glutathione reductase 1.2KU / L, reduced glutathione 6mmol / L, potassium chloride 8g / L, magnesium chloride 0.4 g / L, Tween 20 1ml / L, sucrose 8g / L, sodium azide 1g / L;

[0045] The components in reagent R2 are: Tris buffer 80mmol / L, EDTA 0.9mmol / L, NADPH 1mmol / L, cumene hydroperoxide 0.3mmol / L, Tween 20 1ml / L, sucrose 8g / L, Sodium azide 1g / L.

[0046] The pH value of reagent R1 is 7.0; the pH value of reagent R2 is 9.8;

[0047] The preparation method of the kit in Example 2 is the same as in Example 1.

Embodiment 3

[0049] The components of reagent R1 are: phosphate buffer 50mmol / L, EDTA 1mmol / L, glutathione reductase 2KU / L, reduced glutathione 5mmol / L, potassium chloride 6g / L, magnesium chloride 0.3g / L, Kao B66 1ml / L, tyrosine 1g / L, sodium azide 0.5g / L;

[0050] The components in reagent R2 are: Tris buffer 50mmol / L, EDTA 1mmol / L, NADPH 1mmol / L, cumene hydroperoxide 0.5mmol / L, Kao B66 1ml / L, sucrose 5g / L, azide Sodium 0.5g / L.

[0051] The pH value of reagent R1 is 7.5; the pH value of reagent R2 is 10.0;

[0052] The preparation method of the kit of Example 3 is the same as that of Example 1.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a glutathione peroxidase determination kit, the kit contains a reagent R1 and a reagent R2; the reagent R1 contains buffer solution, EDTA, glutathione reductase, reduced glutathione, potassium chloride, magnesium chloride, surfactant, stabilizer and preservative; and the reagent R2 contains the buffer solution, the EDTA, NADPH, cumene hydrogen peroxide, surfactant, stabilizer and preservative. The invention also discloses a preparation method of the glutathione peroxidase determination kit and application of the glutathione peroxidase determination kit. The kit providedby the invention is a high-sensitivity and excellently stable liquid kit.

Description

technical field [0001] The invention relates to the technical field of biochemical reagent assay, in particular to a glutathione peroxidase assay kit, and also to a preparation method and application of the glutathione peroxidase assay kit. Background technique [0002] Glutathione peroxidase (GSH-Px) is an important peroxide-decomposing enzyme widely present in the body. The active center of GSH-Px is selenocysteine, and its activity can reflect the selenium level in the body. Selenium is a component of the GSH-Px enzyme system. It can catalyze GSH into GSSG and reduce toxic peroxides to non-toxic hydroxyl compounds, thereby protecting the structure and function of cell membranes from interference and damage by peroxides. The reduction of NADPH is linearly related to the activity of glutathione peroxidase. GSH-Px mainly includes four types: cytoplasmic GSH-Px, plasma GSH-Px, phospholipid hydroperoxide GSH-Px and gastrointestinal specific GSH-Px. Cytoplasmic GSH-Px: a tet...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): G01N33/68
CPCG01N33/68
Inventor 隗勇刘安娜张强
Owner 中拓生物有限公司
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com