Glutathione peroxidase determination kit, preparation method of glutathione peroxidase determination kit and application of glutathione peroxidase determination kit
A technology of glutathione peroxide and glutathione, which is applied in the direction of biological testing, material inspection products, etc., can solve the problems of cumbersome steps, high price, waste, etc., and achieve improved sensitivity, improved stability, improved The effect of reactivity
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Embodiment 1
[0039] The components of reagent R1 are: Tris buffer 100mmol / L, EDTA 0.5mmol / L, glutathione reductase 1KU / L, reduced glutathione 5mmol / L, potassium chloride 6g / L, magnesium chloride 0.3g / L, Tween 20 1ml / L, tyrosine 1g / L, sodium azide 0.5g / L;
[0040] The components in reagent R2 are: Tris buffer 100mmol / L, EDTA 0.5mmol / L, NADPH 1mmol / L, cumene hydroperoxide 0.3mmol / L, triton 100 1ml / L, sucrose 5g / L , Sodium azide 0.5g / L.
[0041] The pH value of reagent R1 is 7.0; the pH value of reagent R2 is 9.5;
[0042] Preparation method: (1) Preparation of reagent R1: take an appropriate amount of water, add the raw materials shown in R1 respectively, stir well to dissolve one raw material, then add the next raw material, adjust to the required pH value with hydrochloric acid or sodium hydroxide, and constant volume to required volume. (2) Preparation of reagent R2: Take an appropriate amount of water, add the raw materials shown in R1 respectively, stir well to dissolve one raw mate...
Embodiment 2
[0044] The components of reagent R1 are: Tris buffer 80mmol / L, EDTA 0.8mmol / L, glutathione reductase 1.2KU / L, reduced glutathione 6mmol / L, potassium chloride 8g / L, magnesium chloride 0.4 g / L, Tween 20 1ml / L, sucrose 8g / L, sodium azide 1g / L;
[0045] The components in reagent R2 are: Tris buffer 80mmol / L, EDTA 0.9mmol / L, NADPH 1mmol / L, cumene hydroperoxide 0.3mmol / L, Tween 20 1ml / L, sucrose 8g / L, Sodium azide 1g / L.
[0046] The pH value of reagent R1 is 7.0; the pH value of reagent R2 is 9.8;
[0047] The preparation method of the kit in Example 2 is the same as in Example 1.
Embodiment 3
[0049] The components of reagent R1 are: phosphate buffer 50mmol / L, EDTA 1mmol / L, glutathione reductase 2KU / L, reduced glutathione 5mmol / L, potassium chloride 6g / L, magnesium chloride 0.3g / L, Kao B66 1ml / L, tyrosine 1g / L, sodium azide 0.5g / L;
[0050] The components in reagent R2 are: Tris buffer 50mmol / L, EDTA 1mmol / L, NADPH 1mmol / L, cumene hydroperoxide 0.5mmol / L, Kao B66 1ml / L, sucrose 5g / L, azide Sodium 0.5g / L.
[0051] The pH value of reagent R1 is 7.5; the pH value of reagent R2 is 10.0;
[0052] The preparation method of the kit of Example 3 is the same as that of Example 1.
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