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Blood cell analyzer dilution as well as hemolytic agent

A blood cell analyzer and diluent technology, applied in the field of reagents for medical diagnostic instruments, can solve the problems of pollution, cyanide pollution, harmfulness, etc., and achieve the effect of improving the working environment and reducing injuries

Inactive Publication Date: 2008-10-08
南昌百特生物高新技术股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the biggest disadvantage of this compatible hemolytic agent is that KCN is highly toxic, which not only seriously endangers the health of laboratory workers, but also brings a lot of inconvenience to the operation of the production unit and seriously pollutes the environment.
Although extremely strict and cumbersome measures to reduce cyanide pollution have been formulated clinically, its pollution to the human body and the environment is still unavoidable.
[0004] A cyanide-free hemolytic agent is disclosed in Chinese patent CN99115572.6, but it can only be used in CD series and F series blood cell analysis instruments, and cannot be used in other series of instruments
The product is harmful to the human body when inhaled, and there are some problems in its promotion to practical application, and its application range is also limited
It has not fundamentally solved the problem that blood test personnel, including production personnel, are harmed by toxins, such as azide pollution, cyanide pollution, and cetyl powder in the preparation of operators.

Method used

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  • Blood cell analyzer dilution as well as hemolytic agent
  • Blood cell analyzer dilution as well as hemolytic agent
  • Blood cell analyzer dilution as well as hemolytic agent

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] (1) Preparation of diluent: Add 6.0g of sodium chloride, 5.8g of sodium sulfate, 0.8g of tridimethylurea, 0.2g of copper sulfite, 3g of disodium ethylenediaminetetraacetate, and Dissolve 0.2 g of sodium paxiline, add sodium borate buffer solution to adjust the pH to 7.3, quantify one liter with pure water, and filter.

[0033] (2) Preparation of hemolytic agent: add potassium chloride 3.0g, dodecyltrimethylammonium chloride 50g, octadecyltrimethylammonium bromide 8g, isopropanol 10ml respectively in pure water, dissolve , adjust the pH at 5.3 with 0.34g of carbonate, quantify to one liter with pure water, and filter.

Embodiment 2

[0035] (1) Preparation of diluent: Add 4.0g of sodium chloride, 10g of sodium sulfate, 0.5g of tridimethylurea, 0.5g of copper sulfite, 3g of disodium ethylenediaminetetraacetate, and 3g of diphenhydramine into pure water. Dissolve 0.25 g of xiline sodium, add sodium borate buffer solution to adjust the pH to 7.5, and measure one liter with pure water at the same time, and filter.

[0036] (2) Preparation of hemolytic agent: add potassium chloride 0.9g, dodecyltrimethylammonium chloride 60g, and isopropanol 6ml to pure water respectively, dissolve, adjust pH at 4.5 with sodium bicarbonate, and use Pure water is quantified to one liter and filtered.

Embodiment 3

[0038] (1) Preparation of diluent: 5.0g of sodium chloride, 9g of sodium sulfate, 0.6g of tridimethylurea, 0.3g of copper sulfite, 5g of disodium ethylenediaminetetraacetic acid, and 5g of diphenhydramine were added to pure water. Dissolve 0.5 g of xiline sodium, add sodium borate buffer solution to adjust the pH to 7.8, quantify one liter with pure water, and filter.

[0039] (2) Preparation of hemolytic agent: add potassium chloride 2g, dodecyltrimethylammonium chloride 40g, octadecyltrimethylammonium bromide 4g, isopropanol 9ml respectively in pure water, dissolve, Use carbonate to adjust the pH to 4.0, and at the same time use pure water to quantify to one liter, and filter.

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Abstract

A blood cell analyzer diluent and a hemolytic agent, are characterized in that one litre diluent is provided with 12.0-4.0g of sodium chloride, 2.0-10.0g of sodium sulfate, 0.8-0.5g of 1,3,2-methylol urea, 0.2-0.5g of copper sulfite, 3.0-8.0g of EDTA-2Na, 0.2-0.7g of Piperacillin Sodium, a borate buffering liquid toning the ph value to 7.2-7.8, and the balance is water; one litre hemolytic agent is provided with 0.8-5.0g of potassium chloride, 0-60.0g of dodecyl trimethyl ammonium chloride, 14.0-0g of octadecyl trimethyl ammonium bromide, 6.0-10.0ml of isopropanol, carbonate or alcaine buffering liquid toning the ph value to 7.2-7.8, and the balance is water. The inventive reagent can form stable hemoglobin derivatives, and the absorption spectrum curves are similar when lambada is 540nm, lambada is 504nm, which can satisfy the clinical inspection requirement; the reagent does not contain cyanide, azide, and has non-toxicity, which can effective improve working atmosphere of operating staff, and can reduce harm of toxicant to personal health.

Description

technical field [0001] The invention belongs to the field of reagents for medical diagnostic instruments, in particular to reagents for blood cell analyzers. Background technique [0002] Diluent and hemolyzing reagent are the most commonly used group of reagents for blood analyzers in medical testing, and are key reagents for determining the count or classification of hemoglobin (Hb) and white blood cell (WBC). Under the cooperation of the diluent, the hemolytic agent dissolves red blood cells (RBC) rapidly on the one hand, releases hemoglobin and combines with the hemolytic agent to form a stable hemoglobin derivative for the quantitative determination of Hb, and on the other hand, the white blood cells maintain their original shape and Keep a certain volume for WBC counting, and classify leukocytes into three groups (lymphocytes, neutrophils, intermediate cells) according to their size. In 1996, the International Hematology Standardization Committee recommended the measu...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/49G01N15/10
Inventor 袁水斌刘郁林文玲梅
Owner 南昌百特生物高新技术股份有限公司
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