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Functions of cotton GhMTT8a gene and application of the cotton GhMTT8a gene in brown cellucotton modification by utilization of biological technical means

A brown fiber and gene technology, applied in the field of improving the color of cotton brown fiber, can solve the problems of low lint, limited application of brown cotton, poor pigment stability, etc.

Inactive Publication Date: 2015-01-28
ZHEJIANG ACADEMY OF AGRICULTURE SCIENCES +1
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  • Abstract
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Problems solved by technology

Compared with white cotton, colored cotton varieties still have the following outstanding problems: low fiber specific strength, short fiber length, low clothing content, poor pigment stability, and single color, which limits the application of brown cotton in the textile industry

Method used

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  • Functions of cotton GhMTT8a gene and application of the cotton GhMTT8a gene in brown cellucotton modification by utilization of biological technical means
  • Functions of cotton GhMTT8a gene and application of the cotton GhMTT8a gene in brown cellucotton modification by utilization of biological technical means
  • Functions of cotton GhMTT8a gene and application of the cotton GhMTT8a gene in brown cellucotton modification by utilization of biological technical means

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Experimental program
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Embodiment Construction

[0028] . Materials and Methods

[0029] 1.1 Plant material and growth conditions

[0030] Upland cotton brown fiber Zhecai cotton No. 2, white fiber cotton Zhemian 11, and dark brown fiber cotton P158 were all provided by the Economic Crops Research Office of the Institute of Crop and Nuclear Technology Utilization, Zhejiang Academy of Agricultural Sciences and were sown live in this area in early May 2011. The experimental field of the headquarters of the Hangzhou Institute of the Chinese Academy of Sciences. On the 9th day (after flowering, DPA, Day Post Anthesis), 12d, 15d, 18d, 21d and 24d after cotton flowering, the developing cotton fiber tissue and seed coat tissue were selected for relevant research.

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Abstract

The invention relates to a method of modifying color and luster of cotton brown fiber by utilization of biological technical means, and particularly relates to an application in modification of color and luster of the cotton brown fiber by utilization of a cotton transgenic technology and by utilization of a cotton gene and a plant binary expression vector constructed by the cotton gene. Protein coded by a cotton GhMTT8a gene is provided. The amino acid sequence of the protein is shown as SEQID:NO.2. A plant positive-sense complementary binary expression vector of the cotton GhMTT8a gene is provided. The expression vector adopts the plant expression binary vector pFGC5941 as a skeleton vector, adopts a sequence segment containing a complete protein open reading frame of the cotton GhMTT8a gene as a positive-sense expression structure of the gene, and separately introduces BamHI and XhoI enzyme digestion sites, thus constructing the positive-sense expression vector containing the cotton GhMTT8a gene.

Description

technical field [0001] The invention relates to a method for improving the color of cotton brown fiber by means of biotechnology, in particular to a method for improving the color of cotton brown fiber by using cotton gene and a plant expression binary vector constructed by using cotton transgene technology. Background technique [0002] Natural colored cotton is a special type of cotton whose fiber has natural color. Because of its unique natural color, it does not need bleaching and dyeing in the process of weaving and processing. It is non-polluting to humans and the environment and is comfortable to wear. It is especially suitable for making underwear The real "ecological" and "environmentally friendly" cotton has attracted widespread attention from domestic and foreign cotton breeders and textile and clothing industries, and is known as the most potential ecological textile in the international market in the 21st century. Although the research and development of colored...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/29C12N15/82C07K14/415A01H5/00
Inventor 李付振祝水金邱新棉吴学龙王美兴
Owner ZHEJIANG ACADEMY OF AGRICULTURE SCIENCES
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