Preparation method of targeting and photo-thermal integrated erythrocyte bionic nanoparticles

A red blood cell and nanoparticle technology, applied in the field of preparation of red blood cell bionic nanoparticles, can solve potential cell or genotoxicity, weaken anti-tumor effect and other problems, achieve active targeting, improve therapeutic effect, and increase stability Effect

Inactive Publication Date: 2018-12-28
ZHEJIANG SCI-TECH UNIV
View PDF3 Cites 14 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] However, traditional nanoparticles are easy to adhere to proteins, lipids or other biomolecules to form "protein rings", which will affect their behav

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0045] Example 1

[0046] 1) Take a number of ICR mice of 6 weeks old, use a capillary tube to collect blood from the eyeball and add heparin to the blood for anticoagulation. The obtained whole blood is centrifuged at 4°C, 2000 r / min for 10 min, and the upper serum and other substances are removed. For the blood cell layer, the obtained red blood cell pellet was washed 3 times with cold 1×PBS for use;

[0047] 2) Add 60 times the volume of cold 0.25×PBS to the red blood cell pellet obtained in step 1) for 2 h. Then centrifuge at 10,000 r / min for 15 minutes, discard the upper layer of liquid, the lower layer of light pink precipitate is the red blood cell membrane, and then repeatedly washed with 0.25×PBS for 3 times to make the extracted red blood cell membrane more pure, for use;

[0048] 3) Dissolve doxorubicin in water to prepare a 5% concentration of doxorubicin solution; dissolve polylactic acid-glycolic acid copolymer in dichloromethane to prepare a 2% concentration of polyla...

Example Embodiment

[0058] Example 2

[0059] 1) Take a number of 7-week-old ICR mice, use a capillary tube to collect blood from the eyeball and add heparin to the blood for anticoagulation. The obtained whole blood is centrifuged at 4°C, 2000 r / min for 10 minutes, and the upper serum and other substances are removed. In the blood cell layer, the obtained red blood cell pellet was washed 4 times with cold 1×PBS for use;

[0060] 2) Add 60 times the volume of cold 0.25×PBS to the red blood cell pellet obtained in step 1) to hypotonic for 2.5 h. Then centrifuge at 10000 r / min for 17 minutes, discard the upper layer of liquid, the lower layer of pale pink sediment is the red blood cell membrane, and then repeatedly wash with 0.25×PBS 4 times to make the extracted red blood cell membrane more pure, for use;

[0061] 3) Dissolve doxorubicin in water to prepare a 5% concentration of doxorubicin solution; dissolve polylactic acid-glycolic acid copolymer in dichloromethane to prepare a 2% concentration of pol...

Example Embodiment

[0071] Example 3

[0072] 1) Take several 8-week-old ICR mice, use a capillary tube to collect blood from the eyeballs and add heparin to the blood for anticoagulation. The obtained whole blood is centrifuged at 4°C, 2000 r / min for 10 min, and the upper serum and other substances are removed. For the blood cell layer, the obtained red blood cell pellet was washed 5 times with cold 1×PBS for use;

[0073] 2) Add 60 times the volume of cold 0.25×PBS to the red blood cell pellet obtained in step 1) for 3 h. Then centrifuge at 10,000 r / min for 20 minutes, discard the upper layer of liquid, the lower layer of pale pink precipitate is the red blood cell membrane, and then repeatedly wash with 0.25×PBS for 5 times to make the extracted red blood cell membrane more pure, for use;

[0074] 3) Dissolve doxorubicin in water to prepare a 5% concentration of doxorubicin solution; dissolve polylactic acid-glycolic acid copolymer in dichloromethane to prepare a 2% concentration of polylactic acid-...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention relates to the field of pharmacy and provides a preparation method of targeting and photo-thermal integrated erythrocyte bionic nanoparticles. The method comprises the following steps: firstly, extracting erythrocyte membranes, then loading indocyanine green to a polylactic acid-hydroxyl acetic acid solution, burying adriamycin amycin to the system, and preparing an indocyanine greenpolylactic acid-hydroxyl acetic acid solution buried with adriamycin amycin through a secondary emulsification-solvent evaporation method; then carrying out a reaction on folic chitosan oligosaccharide and the indocyanine green polylactic acid-hydroxyl acetic acid solution to obtain an adriamycin amycin-folic chitosan oligosaccharide-indocyanine green-polylactic acid-hydroxyl acetic acid solution; and finally, extruding the adriamycin amycin-folic chitosan oligosaccharide-indocyanine green-polylactic acid-hydroxyl acetic acid solution into the erythrocyte membranes to obtain the erythrocyte bionic nanoparticles.

Description

technical field [0001] The invention relates to the field of pharmacy, in particular to a preparation method of red blood cell bionic nanoparticles integrating targeting and light and heat. Background technique [0002] Cancer (malignant tumor) is one of the refractory diseases that seriously endanger human health, with high morbidity and mortality every year. Therefore, the study of tumor formation and treatment methods has become the research focus and hotspot of researchers. Polymer nanoparticles are widely used in drug delivery due to their excellent biocompatibility and degradability, high drug loading and drug delivery efficiency, and good cycle stability. system. [0003] However, traditional nanoparticles are easy to adhere to proteins, lipids or other biomolecules to form "protein rings", which will affect their behavior in the body. The rapid clearance in the blood not only weakens its anti-tumor effect, but some nanomaterials also have potential cell or genot...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): A61K41/00A61K9/50A61K9/107A61K47/46A61K47/34A61K47/22A61K47/36A61K31/704A61P35/00
CPCA61K9/1075A61K9/5068A61K31/704A61K41/0052A61K47/22A61K47/34A61K47/36A61P35/00
Inventor 王秉姚舒婷单心怡华称祥胡智文万军民
Owner ZHEJIANG SCI-TECH UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap