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Brush-type hydrazine-polymer functionalized magnetic nano-material, and preparation and application thereof

A magnetic nanometer and polymer technology, applied in the preparation method of peptides, chemical instruments and methods, and the preparation of test samples, to achieve the effects of improving biocompatibility, simple operation, and reducing non-specific adsorption

Active Publication Date: 2015-01-14
DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
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  • Abstract
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  • Claims
  • Application Information

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Problems solved by technology

The brush-type hydrazine polymer functionalized magnetic nanomaterials were prepared by RAFT on the magnetic core and applied to the separation and enrichment of glycopeptides or glycoproteins. The method has not been reported.

Method used

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  • Brush-type hydrazine-polymer functionalized magnetic nano-material, and preparation and application thereof
  • Brush-type hydrazine-polymer functionalized magnetic nano-material, and preparation and application thereof
  • Brush-type hydrazine-polymer functionalized magnetic nano-material, and preparation and application thereof

Examples

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Embodiment Construction

[0032] Example Enrichment of Glycopeptides Using Brush-Type Hydrazine Polymer Functionalized Magnetic Nanomaterials

[0033] Preparation of brush-type hydrazine polymer functionalized magnetic nanomaterials:

[0034] 1) 300 μg Fe 3 o 4 Disperse the particles in 200mL ethanol, 50mL water and 1.4mL concentrated ammonia water, sonicate for 15min, then add 1mL TEOS, stir at room temperature for 10h, wash the product 5 times with 30mL water and ethanol, and place in a vacuum oven at 50°C dry in medium for 24h to obtain Fe 3 o 4 SiO 2 particles;

[0035] 2) Add 200 μg Fe 3 o 4 SiO 2 Disperse the particles in 40 mL of dry toluene. After ultrasonication for 15 minutes, add 8 mL of toluene solution containing 4-chloromethyltrichlorosilane (90 μL) dropwise. After ultrasonication for 5 minutes, slowly drop into 8 mL of toluene solution containing triethylamine (216 μL). , the final reaction solution was refluxed for 24 h under the protection of nitrogen, the product was washed 5...

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Abstract

The invention relates to a brush-type hydrazine-polymer functionalized magnetic nano-material, preparation and application thereof to enriching of glycopeptide or glycoprotein. After a reversible addition-fragmentation chain transfer (RAFT) reagent is introduced into the surface of a nano-material, an organic polymerization reaction functional monomer and an organic polymerization reaction initiator are added, the organic functional monomer is subjected to controllable free-radical polymerization under the regulation and control of the reversible addition-fragmentation chain transfer reagent, so that the brush-type polymer organic-inorganic hybrid nano-material is prepared. The organic-inorganic hybrid nano-material is simple in preparation process and mild in reaction conditions, and is applicable to enriching of glycopeptide or glycoprotein in a complex biological sample.

Description

technical field [0001] The invention relates to the separation and enrichment of glycosylated peptides, in particular to a brush-type hydrazine polymer functionalized magnetic nanometer material and its preparation, as well as its application in the selective separation and enrichment of glycopeptides or glycoproteins. Background technique [0002] As an important post-translational modification, protein glycosylation has important effects on protein folding, conformational stability and activity. Glycosylated proteins are involved in life processes such as protein interaction, immune response, cell communication, and apoptosis. Abnormal changes in glycosylated proteins can be used as an important sign of tumor occurrence and development. [0003] In the analysis of glycosylated proteome, high performance liquid chromatography-mass spectrometry is an effective tool for analyzing glycoprotein or glycopeptide. When the sample is directly analyzed by mass spectrometry, becaus...

Claims

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Application Information

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IPC IPC(8): B01J20/286B01J20/30B01D15/08C07K1/14G01N1/34
CPCB01D15/08B01J20/286B01J20/3085B01J2220/445B01J2220/80C07K1/22G01N1/34
Inventor 邹汉法黄光欧俊杰
Owner DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
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