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Antibody-gold case iron core magnetic nanometer particle synthetic method for cell recognition and separation

A magnetic nanoparticle and nanoparticle technology, applied in the analysis of materials, material magnetic variables, material analysis through optical means, etc., can solve difficult problems such as selectivity reduction, complex synthesis process, long reaction time, etc., and achieve simple detection Intuitive, simple synthesis method, optimized synthesis conditions

Inactive Publication Date: 2008-11-12
CHANGCHUN INST OF APPLIED CHEMISTRY - CHINESE ACAD OF SCI
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  • Summary
  • Abstract
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  • Claims
  • Application Information

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Problems solved by technology

However, in these studies, polylysine or bovine serum albumin complexes are mainly used to stabilize nanoparticles, and then further bind DNA, proteins, antibodies and other functional molecules through covalent bond reactions. The synthesis process is complicated and requires a long time. Reaction time (biopolymer chemistry, Bioconjugate Chem.2004, 15, 482-490), and it is difficult to overcome non-specific adsorption to reduce the selectivity of detection

Method used

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  • Antibody-gold case iron core magnetic nanometer particle synthetic method for cell recognition and separation
  • Antibody-gold case iron core magnetic nanometer particle synthetic method for cell recognition and separation
  • Antibody-gold case iron core magnetic nanometer particle synthetic method for cell recognition and separation

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preparation Embodiment 1

[0028] Preparation Example 1: Synthesis of antibody-gold-shell iron-nuclear magnetic nanoparticles containing 100% epidermal growth factor receptor on the surface for cell recognition and separation, hereinafter referred to as antibody-gold-shell iron-nuclear magnetic nanoparticles

[0029] According to the Lyon method, first utilize 10 milliliters of concentrations to be that the sodium hydroxide of 3.0 mol / liter and 10 milliliters of concentrations all are 10 millimoles / liters ferrous chloride and ferric chloride mixed solution interaction to obtain ferric oxide, further use 10 Milliliter of 0.02 mol / L nitric acid was reacted at 100°C for 1 hour to obtain ferric oxide, and cooled. At room temperature, 10 milliliters of the solution containing the mass concentration of 1% chloroauric acid, 5 mmol / L of sodium citrate and 0.2 mol / L of hydroxylammonium hydrochloride mixed solution was divided 10 times, adding the prepared solution at intervals of 10 minutes each time. Fe2O3 solu...

preparation Embodiment 2

[0032] Preparation Example 2: Synthesis of Antibody-Gold Shell Iron Core Magnetic Nanoparticles Containing 80% Epidermal Growth Factor Receptor Antibody on the Surface

[0033] According to the Lyon method, first utilize 10 milliliters of concentrations to be that the sodium hydroxide of 3.0 mol / liter and 10 milliliters of concentrations all are 10 millimoles / liters of ferrous chloride and ferric chloride mixed solution interaction to obtain ferric oxide, further use 10 Milliliter of 0.02 mol / L nitric acid was reacted at 100°C for 1 hour to obtain ferric oxide, and cooled. At room temperature, 10 milliliters of solutions containing the mass concentration of 1% chloroauric acid, 5 mmol / L of sodium citrate and 0.2 mol / L of hydroxylammonium hydrochloride were divided into 10 times, adding the prepared solution at intervals of 10 minutes each time. Fe2O3 solution to obtain gold-shell iron-core magnetic nanoparticles. Its particle size is 60 nm.

[0034] Dissolve cystylalanylleuc...

preparation Embodiment 3

[0036] Preparation Example 3: Synthesis of Antibody-Gold Shell Iron Core Magnetic Nanoparticles Containing 50% Epidermal Growth Factor Receptor Antibody on the Surface

[0037] According to the Lyon method, first utilize 10 milliliters of concentrations to be that the sodium hydroxide of 3.0 mol / liter and 10 milliliters of concentrations all are 10 millimoles / liters of ferrous chloride and ferric chloride mixed solution interaction to obtain ferric oxide, further use 10 Milliliter of 0.02 mol / L nitric acid was reacted at 100°C for 1 hour to obtain ferric oxide, and cooled. At room temperature, 10 milliliters of solutions containing the mass concentration of 1% chloroauric acid, 5 mmol / L of sodium citrate and 0.2 mol / L of hydroxylammonium hydrochloride were divided into 10 times, adding the prepared solution at intervals of 10 minutes each time. Fe2O3 solution to obtain gold-shell iron-core magnetic nanoparticles. Its particle size is 60 nm.

[0038] Dissolve cystylalanylleuc...

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Abstract

The invention provides a synthesis method of antibody-gold shell and iron nuclear magnetic nanoparticle used for the identification and separation of cells. Peptide chain surface modification method is adopted to cause a peptide chain to stably wrap magnetic nanoparticle and with the interaction of the receptor antibody of epidermal growth factor and the receptor of epidermal growth factor over expressed in specific tumor cell membranes, the cell identification is realized. Almost all specific tumor cells are wrapped by the gold nanoparticle in fuchsia. Under the action of magnetic field, the normal cells and the tumor cells are separated with the separation effect as high as 90 percent. Compared with electronic microscope and fluorescence microscope and the like, of the traditional detection means of cell carriers, the action of the antibody-magnetic nanoparticle and the cells can be observed by the ordinary optical microscope. Samples need no pretreatment and the types of the cells can be judged according to colors; the color rendering of the nanoparticle is stable, the non-quenching property ensures the storage of samples to be detected to be long and the contain of the receptor antibody of epidermal growth factor of the surface of nanoparticles can be controlled by the proportioning of substances in reaction solution.

Description

technical field [0001] The invention relates to a method for synthesizing antibody-gold-shell iron-nuclear magnetic nanoparticles for cell recognition and separation. technical background [0002] Compared with single metal nanoparticles, bimetallic nanoparticles have the advantages of two materials at the same time, so they have wider application fields. Among them, gold-shell iron-core magnetic nanoparticles modified by biomolecules can observe cells in real time and dynamically due to their unique biocompatibility, optical and magnetic properties. The connection between molecules and gold-shell iron-core magnetic nanoparticles uses the optical and magnetic properties of nanoparticles to realize specific cell recognition and separation (Science, Science, 2006, 312, 1027-1030; Biopolymer Chemistry, Bioconjugate Chem.2004, 15, 482-490). However, in these studies, polylysine or bovine serum albumin complexes are mainly used to stabilize nanoparticles, and then further bind ...

Claims

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Application Information

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IPC IPC(8): G01N33/48G01N21/00G01N27/72
Inventor 王振新王金娥孙琳琳
Owner CHANGCHUN INST OF APPLIED CHEMISTRY - CHINESE ACAD OF SCI
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