Polypeptide for inhibiting tumor activity and application of polypeptide

An activity and tumor technology, applied in the field of biomedicine, can solve the problems of slow and complex drug development targeting p53, and achieve the effect of increasing permeability and inhibiting activity

Pending Publication Date: 2020-11-10
THE NAT CENT FOR NANOSCI & TECH NCNST OF CHINA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, although the research on the p53 tumor suppressor gene is relatively in-depth, the development of drugs targeting p53 is still very slow due to the complexity of its mutations.

Method used

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  • Polypeptide for inhibiting tumor activity and application of polypeptide
  • Polypeptide for inhibiting tumor activity and application of polypeptide
  • Polypeptide for inhibiting tumor activity and application of polypeptide

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0093] This example is used to illustrate the preparation method of the palmitic acid modified polypeptide of the present invention.

[0094] First, 1 mM palmitic acid, condensing agent diisopropylcarbodiimide (DIC, 1 mM) and racemic agent HOBT (2 mM) were dissolved in N,N-dimethylformamide (DMF) solution at 37 °C Mix and react with the exposed amino group at the N-terminus of the polypeptide under conditions for 1 hour.

[0095]Secondly, during the reaction process, use the ninhydrin detection method to detect whether the reaction is complete every 15 minutes. The specific method includes: adding 20 μL of ninhydrin reagent to the EP tube, dipping a little resin into the solution with the tip of the pipette, and putting the EP tube into the solution. Put it into 100°C water, and observe the color change of the solution after 1 min. If there is no color change in the solution, it indicates that the palmitic acid is successfully coupled to the polypeptide. Thirdly, the final pr...

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Abstract

The invention provides a polypeptide for inhibiting tumor activity. The amino acid sequence of the polypeptide is XYGRKRRQRRR, the N-terminal X of the polypeptide is the sequence consisting of 10-50,preferably 15-30, more preferably 18-27 amino acids, and the N terminal can be modified or not modified, and the invention also provides an application of the polypeptide. The polypeptide disclosed bythe invention can interact with p53 wild type and mutant type tumor cells, so that the permeability of tumor cell membranes is improved, and the activity of the tumor cells is inhibited. The polypeptide has the potential of serving as a medicine for treating or assisting in treating tumors, and particularly serves as a medicine for treating or assisting in treating diseases such as lung cancer, breast cancer, osteogenic sarcoma, ovarian cancer and/or colon cancer.

Description

technical field [0001] The present invention belongs to the technical field of biomedicine, and specifically relates to a polypeptide for inhibiting tumor activity and its application, and the application for inhibiting the activity of p53 wild-type and mutant tumor cells. Background technique [0002] Tumor has become the leading cause of human death worldwide. According to statistics, by 2030, there will be more than 21 million cancer patients worldwide. Studies have shown that tumor suppressor genes (tumor suppressor genes) are a class of genes that exist in normal cells and can inhibit cell growth and have potential tumor suppressor effects. Tumor suppressor genes and proto-oncogenes mutually restrict and jointly maintain and regulate cell growth, proliferation and differentiation. When the tumor suppressor gene is mutated, deleted or inactivated, it can cause malignant transformation of cells and lead to the occurrence of tumors. So far, people have isolated and iden...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K14/47C07K1/20C07K1/107C12N15/11A61K38/16A61P35/00
CPCA61K38/00A61P35/00C07K14/4703
Inventor 王琛杨延莲梁伟李浩方小翠
Owner THE NAT CENT FOR NANOSCI & TECH NCNST OF CHINA
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