In-situ electrochemical immunological detecting method of tumor cell surface antigen

An in situ electrochemical and tumor cell technology, applied in the field of in situ determination of tumor cell surface antigens, can solve the problems of unreported in situ immunoassay of differentiation antigens on the surface of malignant tumor cells, and achieve good chemical stability and biocompatibility High performance, good application prospects, and fast analysis speed

Inactive Publication Date: 2005-03-02
NANJING UNIV
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Problems solved by technology

At present, there is no report on the in situ immunoassay of differentiatio

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  • In-situ electrochemical immunological detecting method of tumor cell surface antigen
  • In-situ electrochemical immunological detecting method of tumor cell surface antigen

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Embodiment Construction

[0031] Taking the determination of P-gp antigen on the surface of K562 leukemia cells (K562 / ADM cells) induced by doxorubicin as an example, of course general samples can also be detected by the method of the present invention:

[0032] 1. K562 / ADM cell fixation

[0033] (1) Electrode polishing: Polish the 4mm disc-shaped glassy carbon electrode with 1.0, 0.3 and 0.05 μm α-alumina suspensions on the suede to become a mirror surface, rinse with secondary water, and then use 1:1 nitric acid, Ultrasonic cleaning with acetone and secondary water to obtain a fresh and clean electrode surface.

[0034](2) Electrode surface pretreatment: After the polished glassy carbon electrode was oxidized and electrolyzed at a constant potential of +1.75V in a phosphate buffer solution of pH 5.0 for 300 seconds, it was then sequentially oxidized and electrolyzed at +0.3~+1.3V and +0.3~ Cycle scanning within the potential range of -1.3V until a stable current value is obtained. The electrodes we...

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Abstract

The invention provides a new in situ electrochemical immunity detection method for tumor cell surface divided antigen. It fixes the tumor cell on the gold glue-chitosan film bionic interface through chemical decoration technology. The invention uses the character that the electrochemical technology is convenient, quick and cheap, combines the high selectivity of immunity analysis and high sensitivity method, generates molecular with electrochemical activity by combining fixed tumor cell surface and enzyme bottom catalyst reaction, and realizes the in situ ampere detection of tumor cell film surface antigen. The invention integrates the preparation and detection onto an electrode surface, the cell quantity and the other reagent are little, the time is short, and the cost is low. The operation is simple, the cost is low, and the analyzing speed is quick.

Description

technical field [0001] The invention relates to an immunoassay method for cell surface antigens, in particular to an in situ assay method for tumor cell surface antigens established in combination with electrochemical analysis techniques. Background technique [0002] The rising trend of morbidity and mortality of malignant tumors is directly threatening human health. Its early diagnosis and treatment are the key to improving the survival rate of cancer patients. The research on early diagnosis and curative effect observation methods has become an important frontier field of cancer prevention and treatment research. one. Tumor-associated antigens are some substances that are produced due to changes in the metabolism of cancer cells and can indicate the process of carcinogenesis. Early detection of malignant tumors has become the main means of tumor diagnosis and condition monitoring. The use of immunocytochemical methods to detect and locate t...

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Application Information

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IPC IPC(8): G01N27/327G01N27/404G01N33/535G01N33/554
Inventor 鞠熀先杜丹
Owner NANJING UNIV
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