Preparation method and application of magnetic nano microcarrier for wrapping tumor cell membrane

A magnetic nanometer and tumor cell technology, applied in cell culture active agents, biochemical equipment and methods, nanotechnology, etc., can solve the problems of antigen loss and low antigen presentation efficiency, achieve easy enrichment and separation, and enhance tumor immunity Therapeutic and practical effects

Inactive Publication Date: 2020-07-31
NANJING DRUM TOWER HOSPITAL
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  • Summary
  • Abstract
  • Description
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AI Technical Summary

Problems solved by technology

[0005] The purpose of the present invention is to solve the problems of low efficiency of traditional tumor antigen presentation a...

Method used

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  • Preparation method and application of magnetic nano microcarrier for wrapping tumor cell membrane
  • Preparation method and application of magnetic nano microcarrier for wrapping tumor cell membrane
  • Preparation method and application of magnetic nano microcarrier for wrapping tumor cell membrane

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preparation example Construction

[0035] (1) Fe 3 o 4 Preparation of Magnetic Nanoparticles

[0036] Accurately weigh the compound 0.26g ferric chloride, 0.40g poly(4-styrenesulfonic acid-copolymerization-maleic acid) sodium salt, 1.20g sodium acetate, mix and dissolve in 60μL ultrapure water and 16mL ethyl alcohol In the diol, use a magnetic stirrer to stir vigorously at room temperature for about half an hour until the solution is uniformly yellow, then add 0.24 g of sodium hydroxide to the above solution, stir continuously until it is completely dissolved, and then mix the above solution with 20 mL of Mix tetrafluoroethylene, put it in a stainless steel autoclave, and undergo a hydrothermal reaction at 190°C for 9 hours. After the reaction is over, wait for the solution to cool to room temperature, and use a magnet to 3 o 4 Magnetic nanoparticles were separated, and ethanol and water were added at a ratio of 1:1 by volume, and the Fe 3 o 4 Nanoparticles are washed three times with the above-mentioned m...

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Abstract

The invention discloses a preparation method and application of a magnetic nano microcarrier for wrapping a tumor cell membrane. The method is based on the tumor cell membrane, and the surface of a ferroferric oxide nano particle modified by silicon dioxide is wrapped with the cell membrane derived from a tumor cell through a membrane-nano coextrusion and centrifugation method, so that the Fe3O4@SiO2 magnetic nano microcarrier (CMNPs) wrapped with the tumor cell membrane is formed. The magnetic nano microcarrier contains a tumor cell membrane surface antigen, has superparamagnetism, and can beenriched and purified by a magnetic field, so that the magnetic nano microcarrier can stimulate activation of immune cells in vitro or is injected into a body to be captured by macrophages to improvea tumor specific antigen, and the anti-tumor effect is enhanced. The preparation method of the microcarrier based on the cell membrane has the advantages of simplicity and easiness in operation, lowcost, wide application and the like.

Description

technical field [0001] The invention relates to the field of biomedical materials, in particular to a preparation method and application of a magnetic nano-microcarrier wrapping tumor cell membranes. Background technique [0002] Tumor immunotherapy, which effectively kills tumors by stimulating the body's own immune system, has become the hope of "curing" cancer in the future. In recent years, some emerging cancer immunotherapies, including monoclonal antibody (mAb) immune checkpoint blockade, adoptive cell therapy and cancer vaccines, have changed the traditional means of cancer treatment and achieved encouraging clinical efficacy. Among them, adoptive cell therapy is a therapy with clinical application prospects, that is, to separate autologous immune cells from the human body, activate them in vitro or increase their tumor specificity, and then infuse them back into patients to directly kill tumor cells or stimulate the body to produce Tumor-specific immune responses. ...

Claims

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Application Information

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IPC IPC(8): A61K39/00A61K9/50A61K47/02A61P35/00A61P37/04B82Y5/00B82Y25/00B82Y40/00C12N5/078
CPCA61K9/5094A61K39/0011A61K47/02A61K2039/575A61P35/00A61P37/04B82Y5/00B82Y25/00B82Y40/00C12N5/0634C12N2501/998
Inventor 赵远锦伍丹王月桐
Owner NANJING DRUM TOWER HOSPITAL
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