Cell lysis solution, kit and application of cell lysis solution to preparation of tumor whole cell antigen loaded DC tumor vaccine

Abstract

The invention discloses a cell lysis solution, a kit and application of the cell lysis solution to the preparation of a tumor whole cell antigen loaded DC tumor vaccine. The cell lysis solution comprises a buffer system component, an isotonic system component, a lysis cell membrane component, a protein stabilizer and a protease inhibitor, wherein the lysis cell membrane component is selected from a compound castor oil sulfonate or ricinoleate. The cell lysis solution has the advantages that the compound castor oil sulfonate or ricinoleate can mildly induce tumor cell membrane lysis without destroying the high-level conformation of cell membrane protein and cytoplasm protein, the cell lysis solution prepared by the compound castor oil sulfonate or ricinoleate can keep the antigenicity of lysate to the maximum extent, and the lysate sensitized DC vaccine obtained by co-culturing DC with the tumor cell lysate split by the cell lysis solution has an excellent antitumor effect; compared with a freezing-dissolving lysis method commonly used in the prior art, the cell lysis solution is evidently better than a traditional lysis solution and has a tumor preventing and treating effect close to the freezing-dissolving lysis.

Description

technical field [0001] The invention belongs to the field of tumor immunity and relates to a tumor cell lysate, in particular to a cell lysate, a lysate kit and an application in preparing a DC tumor vaccine loaded with tumor whole cell antigens. Background technique [0002] Immunotherapy is the fourth tumor treatment method after surgery, radiotherapy, and chemotherapy. In the comprehensive treatment mode of tumors, immunotherapy plays an important role in improving the cure rate of tumors, improving the quality of life of patients, and prolonging survival. In tumor immunity, tumor antigens recognized by T lymphocytes are not presented by tumor cells, but by antigen-presenting cells. Dendritic cells (DC) are the most powerful antigen-presenting cells known in the human body and the only antigen-presenting cells that can activate resting T cells, and have a strong ability to activate helper and cytotoxic T cells. Dendritic cell tumor vaccine is one of the fourth modes of t...

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Problems solved by technology

Compared with the four methods, ultrasonic treatment is the most severe, which is likely to cause DNA breakage and protein denaturation; enzymatic cleavage is the most likely to destroy tumor cell lysate and reduce antigenicity in lysate-sensitized DC vaccine; repeated freeze-thaw method is the mildest , the lysis efficiency is also high, but freeze-thaw lysis requires repeated freeze-thaw cycles of tumor cells at -80°C and 37°C for more than 4 cycles, the operation is time-consuming, and it is not easy to commercialize and standardize; The enzymatic lysis method is more destructive to tumor cell lysates, and it is faster and more convenient than the freeze-thaw lysis method. The only disadvantage is that it will still cause damage to the advanced conformation of the antigenic components in the tumor cell lysate to a certain extent, reducing the antigenicity.

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