Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Multifunctional multimode tumor-specific targeting phase-change nano-microsphere photoacoustic contrast medium and application thereof

A tumor-specific, nano-microsphere technology, applied in the field of multi-functional multi-modal tumor-specific targeted phase-change nano-microsphere photoacoustic contrast agents, can solve the problem of weak absorption of light and other problems

Active Publication Date: 2017-01-04
CHONGQING MEDICAL UNIVERSITY
View PDF0 Cites 23 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0016] Although liquid fluorocarbon has the property of "light absorber", it absorbs light weaker than traditional light absorbing contrast agents

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Multifunctional multimode tumor-specific targeting phase-change nano-microsphere photoacoustic contrast medium and application thereof
  • Multifunctional multimode tumor-specific targeting phase-change nano-microsphere photoacoustic contrast medium and application thereof
  • Multifunctional multimode tumor-specific targeting phase-change nano-microsphere photoacoustic contrast medium and application thereof

Examples

Experimental program
Comparison scheme
Effect test

example 1

[0055] Example 1 Preparation of folic acid targeting polylactic acid glycolic acid (PLGA-PEG-FA)

[0056] 1. Experimental method

[0057] ① PLGA-COOH and NHS are coupled to synthesize PLGA-NHS: Dissolve 0.04MM PLGA-COOH in 4ml DCM, take 0.4MM NHS and 0.3MM DCC and dissolve them in 2ml DCM, mix the two, place in a shaker and shake slowly Overnight, add 80ML ice methanol and ether mixed solution (methanol and ether volume ratio 1:1) and shake well, and let stand overnight in a refrigerator at 4°C. When a large amount of precipitation appears, collect the precipitate by centrifugation and vacuum dry to obtain PLGA-NHS.

[0058] ②Synthesis of monoamino-protected NH2-PEG-NH-BOC: Diamino PEG is dissolved in 50ml NaHCO3, pH 7.50, and the final concentration is 1mg / ml; di-tert-butyl dicarbonate (BOC reagent) is dissolved in 10ml DMSO, the final concentration is It is 0.1mg / ml. Mix according to the mass ratio of PEG and BOC of 5:1, stir and react overnight at room temperature. After BOC, i...

example 2

[0066] Example 2 Preparation of folic acid-targeted phase-change gold-loaded multi-functional multi-modal photoacoustic contrast agent (GNP / PFP / PLGA-PEG-FA and GNP / PFH / PLGA-PEG-FA)

[0067] 1. Experimental method

[0068] ①Take 25mg PLGA+3mg nano-gold+3ml dichloromethane to dissolve completely;

[0069] ②Add 200ulPFP or PFH respectively;

[0070] ③ Vibrate 100W in an ice salt water bath for 1min. So far, the initial emulsion is obtained;

[0071] ④ Take another 75 mg of the targeting shell material PLGA-PEG-FA, and add 2ml of dichloromethane to dissolve to obtain a solution;

[0072] ⑤ After adding the solution obtained in step 4 to the primary emulsion, shake vigorously for 2.5 min;

[0073] ⑥ Under continuous stirring, slowly add 3ml of 5% PVA solution respectively, and vibrate 75W in an ice salt water bath for 2min;

[0074] ⑦Magnetic stirring for 4h at room temperature to make the dichloromethane volatilize as fully as possible;

[0075] ⑧ Transfer them to centrifuge tubes, centrifuge ...

example 3

[0080] Example 3 GNP / PFP / PLGA-PEG-FA in vitro tumor cell specific targeting study

[0081] Human ovarian cancer cells (SKOV3 cells) with high expression of folate receptor were used as experimental materials. Take SKOV3 cells in logarithmic growth phase, add 200μl of SKOV3 cell suspension with adjusted concentration into 6 EP tubes, each tube has 16,000 cells, and use FITC-labeled GNP / PFP / PLGA-PEG-FA ( Expressed as GNP / PFP / PLGA-PEG-FA-FITC) diluted to 2×104 pieces / ml, 4×104 pieces / ml, 8×104 pieces / ml, 16×104 pieces / ml, 32×104 pieces / ml Five different concentrations, add 50μl to each tube. Add 50μl FITC to the remaining 1 tube as a negative control, and incubate at 37°C for 30 minutes. Centrifuge, remove the supernatant, and detect positive cells on a flow cytometer within 2 hours after resuspending in PBS. When SKOV3 cells were incubated with FITC, no positive cells with FITC fluorescence were detected by flow cytometry. With the addition of GNP / PFP / PLGA-PEG-FA photoacoustic c...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
molecular weightaaaaaaaaaa
molecular weightaaaaaaaaaa
particle diameteraaaaaaaaaa
Login to View More

Abstract

The invention discloses a multifunctional multimode tumor-specific targeting phase-change nano-microsphere photoacoustic contrast medium, structurally comprising, from outside to inside, a ligand that is positioned on the surface of nano-microspheres and specifically binds with a tumor cell membrane surface receptor; the ligand is connected with shell membrane material through linker molecules, and the shell membrane material wraps photo-absorbers and phase-change molecules to the core. A preparation method of the medium includes: linking the ligand to the shell membrane material through the linker molecules by a 'five-step process' to prepare a targeting shell; wrapping the photo-absorbers and phase-change molecules to the core of the nano-microspheres by a special double-emulsification process, while making sure the targeting ligand is positioned on the surface of the nano-microspheres. In-vivo and in-vitro experiments indicate that the photoacoustic contrast medium may specifically bind with tumor cells to experience phase change; there are strong photoacoustic, ultrasonic and X-ray signals, and the medium is applicable to photoacoustic imaging, ultrasonic imaging and computed tomography.

Description

Technical field [0001] The invention relates to a multifunctional multi-modal tumor-specific targeting phase-change nano-microsphere photoacoustic contrast agent, and a preparation method and application thereof, belonging to the technical field of imaging diagnostics and therapeutic drugs. Background technique [0002] The key to tumor treatment is early diagnosis. Commonly used image detection methods such as ultrasound, MRI, CT, etc. play their own different advantages in the early diagnosis of tumors. Regardless of the imaging technique, it is important for tumors to find changes in their structure, metabolism, and biochemical information in their early stages. With the emergence of various contrast agents, various imaging techniques have greatly improved the detection of early tumors. However, ordinary contrast agents lack affinity and specificity for tumor tissues and cannot effectively reside in tumor tissues. They can only be used in transient arterial phases. The mediu...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): A61K49/00A61K49/04A61K49/22A61K9/16
Inventor 李茂萍王志刚冉海涛郑元义李攀张炜阳何坤燕
Owner CHONGQING MEDICAL UNIVERSITY
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com