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DNA origami structure, closing and releasing method of DNA origami structure and application of DNA origami structure as drug delivery system

An origami structure and DNA chain technology, applied in the field of DNA nano-drug loading, can solve the problems of low efficiency and limited loading of drugs, and achieve the effect of improving loading efficiency

Inactive Publication Date: 2020-12-29
HUAZHONG UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The invention solves the technical problem of limited drug loading and low efficiency in the DNA origami structure in the prior art, and proposes a drug delivery system based on nano-gold controlled opening and closing of the DNA origami structure. The DNA origami structure of the present invention The drug delivery system is composed of four triangular origami and a nano-gold particle. Through the principle of complementary base pairing and strand displacement reaction, the dynamic control of the DNA origami structure from two-dimensional to three-dimensional is realized.

Method used

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  • DNA origami structure, closing and releasing method of DNA origami structure and application of DNA origami structure as drug delivery system
  • DNA origami structure, closing and releasing method of DNA origami structure and application of DNA origami structure as drug delivery system
  • DNA origami structure, closing and releasing method of DNA origami structure and application of DNA origami structure as drug delivery system

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Effect test

Embodiment 1

[0088] Step by step method:

[0089] (1) Specific preparation of the unlocked central triangle origami: 1.6nM M13mp18 chain and staple chain were mixed at a ratio of 1:10 in 1×TE, 11mM MgCl 2 Mix in the buffer, anneal from 90°C to room temperature, and assemble into triangular DNA Origami. One capture strand is extended from each hollow of the triangular DNA Origami, and 6 connecting strands are extended from each outer edge of the triangular DNA Origami according to the design;

[0090] (2) Use gel electrophoresis to purify the unlocked central triangular origami, and then mix the purified unlocked central triangular origami with gold nanoparticles with a diameter of 20 nm grown into Au-SH chains at a ratio of 1:1, React at room temperature for 2 hours, and use gel electrophoresis to purify to obtain a central triangular origami that captures gold nanoparticles;

[0091] (3) Specific preparation of the upper lock triangle origami: 1.6nM M13 mp18 chain and staple chain were m...

Embodiment 2

[0095] One-step closure:

[0096] (1) Specific preparation of the unlocked central triangle origami: 1.6nM M13mp18 chain and staple chain were mixed at a ratio of 1:10 in 1×TE, 11mM MgCl 2 Mix in the buffer, anneal from 90°C to room temperature, and assemble into triangular DNA Origami. One capture strand is extended from each hollow of the triangular DNA Origami, and 6 connecting strands are extended from each outer edge of the triangular DNA Origami according to the design;

[0097] (2) Specific preparation of unlocked triangular origami: 1.6nM M13mp18 chain and staple chain at a ratio of 1:10 in 1×TE, 11mM MgCl 2 Mix in the buffer, anneal from 90°C to room temperature, and assemble into a triangular DNA Origami. One capture strand is extended from each hollow of the triangular DNA Origami, and 6 connecting strands are extended from one outer side of the triangular DNA Origami design.

[0098] (3) The unlocked center triangle origami and the unlocked side triangle origami w...

Embodiment 3

[0101] Tetrahedral opening: Mix the tetrahedral structure purified by gel electrophoresis with the Release chain at a ratio of 1:5, react at room temperature for 2 hours, and then use AFM (atomic force microscope) to observe the planar tetrahedral structure and isolation. of gold nanoparticles.

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Abstract

The invention relates to a DNA origami structure, a closing and releasing method of the DNA origami structure and an application of the DNA origami structure as a drug delivery system, and belongs tothe technical field of DNA nano drug delivery. The DNA origami structure is a DNA tetrahedron and comprises three side triangular origamis and a central triangular origami, and three sides of the central triangular origami are connected with the three side triangular origamis respectively; skeleton chains of the central triangular origami and the side triangular origamis are annular single-helix DNA chains of M13 bacteriophage; the central triangular origami and the side triangular origamis are occluded through base complementary pairing of respective internal staple chains and respective skeleton chains; the central triangular origami is complementarily paired with connecting staple chains of the side triangular origamis through a connecting staple chain; and the central triangular origami and the side triangular origamis capture nano-gold particles through capture chains, and the side triangular origamis and the central triangular origami form a three-dimensional tetrahedral origamistructure through traction of the nano-gold particles. According to the invention, the drug loading efficiency is improved.

Description

technical field [0001] The present invention relates to the technical field of DNA nano drug loading, more specifically, to a DNA origami structure, its closure and release method and its application as a drug delivery system, especially to a tetrahedral DNA origami structure based on nano-gold particle controlled opening and closing, Its closure and release method and its application as drug delivery system. Background technique [0002] Due to the high level of structural programmability and good biocompatibility of DNA nanostructures, DNA nanostructures are very promising for use as nanocarriers for drug delivery. The literature "Aptamer-Conjugated DNAIcosahedral Nanoparticles As a Carrier of Doxorubicin for Cancer Therapy" was the first to report drug delivery carriers based on DNA nanostructures. The author Microsuger Chang used primer sequences to intermolecularly construct DNA icosahedral nanoparticle as a carrier of doxorubicin. Vector, an aptamer-conjugated DNA ico...

Claims

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Application Information

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IPC IPC(8): C12N15/11C12N15/10A61K9/51A61K47/26A61K47/02
CPCC12N15/11C12N15/10A61K9/5123A61K9/5115C12Q2537/137
Inventor 潘林强廖康超徐飞
Owner HUAZHONG UNIV OF SCI & TECH
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