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Method for detecting polymorphism of single nucleotide with gold nanoparticles

A single nucleotide polymorphism and nano-gold particle technology, which is applied in the direction of biochemical equipment and methods, microbial measurement/inspection, etc., can solve the problem of no discovery and achieve the effect of low cost

Inactive Publication Date: 2011-01-26
SHANGHAI JIAO TONG UNIV +1
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Problems solved by technology

[0006] In the further search of the prior art literature, there is no finding: "Using 5nm particle size colloidal gold attached to the nano-origami structure imitating the shape of a Chinese map as a marker, and applying the toehold principle" to detect single nucleotide polymorphisms Reports in related literature

Method used

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Embodiment

[0035] Detection conditions:

[0036] In this embodiment, the shape of the map of China constructed by DNA origami is formed by repeated folding of the scaffolding long chain (single-stranded DNA of M13mp18 bacteriophage, up to 7249 bases), and the pattern of the map comes from the 229 short staple chains are determined. The characteristic of this figure is that the 229 staple chains are more than two hundred addressable potential reaction sites. Any point on the template can be precisely positioned using the figure itself as a reference. There is no need for an index label, and any one of the 229 short DNA single strands can extend out of the nucleotide sequence as a capture probe to hybridize with the complementary nucleotide sequence labeled with nanoparticles, as the label of the nanoparticles on the origami pattern Points, under the atomic force microscope (Atomic Force Microscope, AFM), present prominent bright spots for observation.

[0037] In this example, the map of...

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Abstract

The invention relates to a method for detecting the polymorphism of single nucleotide with gold nanoparticles in the nanotechnology field. The method comprises the following steps of: constructing an unsymmetrical two-dimensional pattern by utilizing a DNA (Deoxyribonucleic Acid) origami technology to prepare a DNA origami chip; preparing a colloidal gold probe; hybridizing a DNA short chain and the colloidal gold probe; connecting the particle-size colloidal gold probe and the DNA origami chip in the unsymmetrical two-dimensional pattern; and detecting the polymorphism of the single nucleotide. The invention has the advantages of simpleness, low cost, and the like without strict experimental conditions and complicated enzyme reactions and can realize a DNA hybridization process in a nano-scale liquid-phase reaction and achieve high-sensitivity biomedical detection.

Description

technical field [0001] The invention relates to a detection method in the field of nanotechnology, in particular to a method for detecting single nucleotide polymorphisms by utilizing nano gold particles with a diameter of 5nm constructed on an asymmetric two-dimensional DNA origami chip. Background technique [0002] Nanogold refers to tiny particles of gold with a diameter of 1-100nm, generally a colloidal solution formed by dispersing in water, so it is also called colloidal gold. A certain chemical substance is adsorbed on the surface of the gold nanoparticle, and the single gold nanoparticle is very stable. Gold nanoparticles have high electron density, quantum size effect, small volume effect and surface effect, and can be combined with macromolecules through electrostatic attraction, hydrophobic effect and other forms of interaction to form stable colloidal gold probes. In the past 40 years, gold nanoparticles have been widely used in biotechnology such as immunocyto...

Claims

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Application Information

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IPC IPC(8): C12Q1/68
CPCC12Q1/68
Inventor 贺林李璨冯晓录陈培华
Owner SHANGHAI JIAO TONG UNIV
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