Nucleic acid structure of which interchain exchange is achieved by support DNA (deoxyribonucleic acid) and synthesis method thereof

A synthesis method and strand exchange technology, which can be used in DNA preparation, recombinant DNA technology, DNA/RNA fragments, etc., and can solve problems such as difficulty in precise control of size and shape.

Pending Publication Date: 2016-05-25
TONGJI UNIV +1
View PDF4 Cites 6 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Subsequently, researchers constructed different primitive modules, such as DX (double-crossover), TX (triple-crossover) modules, cross modules and symmetrical modules, and assembled them to obtain a variety of graphic structures (t

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Nucleic acid structure of which interchain exchange is achieved by support DNA (deoxyribonucleic acid) and synthesis method thereof
  • Nucleic acid structure of which interchain exchange is achieved by support DNA (deoxyribonucleic acid) and synthesis method thereof
  • Nucleic acid structure of which interchain exchange is achieved by support DNA (deoxyribonucleic acid) and synthesis method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0107] like Figure 13 Shown, the preparation process that the present invention relates to comprises the following steps:

[0108] 1. Sample preparation:

[0109] The short-chain DNA was synthesized by Bioneer Company, and all the short-chain DNA needed to form a single nucleic acid structure was absorbed with equal volume, and DEPC water was added to make the concentration of each short-chain DNA in the final mixed solution 500nM. The dilution factor is based on the starting concentration from the manufacturer's specification sheet and no additional internal corrections are performed, so the stoichiometry of short-strand DNA does not need to be precisely controlled.

[0110] The M13mp18 in the scaffold DNA was purchased from NEB Company, and an appropriate amount of pure water was added to make the initial scaffold DNA concentration 100 nM.

[0111] 2. Annealing reaction

[0112] Mix 10nM scaffold DNA with 200nM short-chain DNA in a buffer solution (10mM Tris, pH8.0, 2mM ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention discloses a nucleic acid structure of which the interchain exchange is achieved by a support DNA (deoxyribonucleic acid). The nucleic acid structure is a two-dimensional or three-dimensional nucleic acid structure with controllable size and shape, which is formed by achieving interchain exchange by the support DNA. The nucleic acid structure comprises a set of parallel double helices connected by interchain exchange and a single-chain DNA segment, wherein the double helices are formed by carrying out folding and interchain exchange by the support DNA, carrying out specific matching with a plurality of short-chain DNAs and carrying out annealing reaction; and the single-chain DNA is the part of the support DNA which does not form the double helices. The invention provides the multiple nucleic acid structures and synthesis methods thereof. The nucleic acid structure is a variant technique based on DNA paper folding. The invention aims to form the nucleic acid structure with controllable size, shape, complexity and modification by achieving interchain exchange by using the support DNA. The nucleic acid structure is completed by the support DNA/short-chain DNA self-assembly process, and has the advantages of higher yield and simple design; and the predicated two-dimensional and three-dimensional structures can be simultaneously synthesized.

Description

technical field [0001] The invention relates to a nucleic acid structure and a synthesis method thereof in the field of DNA nanotechnology. More specifically, it relates to methods for synthesizing two-dimensional and three-dimensional nucleic acid structures with controllable size, shape, and complexity at the nanoscale using strand exchange achieved by scaffold DNA, and the nucleic acid structures themselves. Based on the addressability of each short strand of DNA in the resulting structure, nucleic acid structures with specific cavities and modifications can be obtained. Background technique [0002] In the 1980s, Seeman first proposed that DNA could be assembled into a complex spatial structure using the principle of DNA base complementary pairing, creating a new field of using DNA as a nanoscale construction material rather than a carrier of genetic information, and named it as DNA nanotechnology. Subsequently, researchers constructed different primitive modules, such...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): C12N15/11C12N15/10
CPCC12N15/11C12N15/102
Inventor 陈瑞鹏开明轩崔妍弭永利魏迪明
Owner TONGJI UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap