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Method used for detecting acrylamide concentration of solutions

A technology of acrylamide and concentration, applied in the field of chemical detection, achieves the effects of simple and convenient operation, lower detection cost, and improved sensitivity

Active Publication Date: 2015-11-11
NINGBO XIANAN CHEM
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Therefore, the characteristic single-stranded DNA can be used as a sensing platform to detect acrylamide by electrochemical technology. So far, there are no reports on the use of characteristic single-stranded DNA modified glassy carbon electrodes for the detection of acrylamide.

Method used

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  • Method used for detecting acrylamide concentration of solutions
  • Method used for detecting acrylamide concentration of solutions

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Embodiment 1

[0042] The invention provides a method for detecting the concentration of acrylamide in a solution, comprising:

[0043] Use a three-electrode system to detect acrylamide in the liquid to be detected by differential pulse voltammetry, and obtain the concentration of acrylamide in the liquid to be detected according to the linear equation of acrylamide, wherein the working electrode in the three-electrode system is carboxylated graphene And the characteristic single-stranded DNA modified glassy carbon electrode, the sequence of the characteristic single-stranded DNA is 5'-AAAAAAAAAGGAAAAAAAAA-(CH 2 ) 6 -SH-3'. The three-electrode system consists of a working electrode, a reference electrode and an auxiliary electrode.

[0044] The present invention uses differential pulse voltammetry to detect acrylamide in the liquid to be detected. Differential pulse voltammetry superimposes a continuous pulse with constant amplitude and fixed pulse width on the linear scanning waveform. Du...

Embodiment 2

[0066] Electrochemical workstation: CHI760E;

[0067] Three-electrode system: the working electrode is a glassy carbon electrode modified by carboxylated graphene and characteristic single-stranded DNA, and the sequence of characteristic single-stranded DNA is 5'-AAAAAAAAAGGAAAAAAAAA-(CH 2 ) 6 -SH-3', its 3' end is partially modified with mercapto groups; the auxiliary electrode is a platinum electrode; the reference electrode is an Ag / AgCl electrode;

[0068] Buffer solution: a phosphate buffer solution with a pH value of 7.0 and a concentration of 0.2mol / L;

[0069] Standard stock solution: Acrylamide standard solution with a concentration of 2 μmol / L;

[0070] Treatment of glassy carbon electrodes before modification: place the glassy carbon electrodes on polishing cloths containing polishing powders with particle sizes of 1 μm, 0.3 μm, and 0.05 μm in sequence, and then place the glassy carbon electrodes in sequence in acetone with a concentration of Sonicate 0.5mol / L su...

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Abstract

The invention discloses a method used for detecting acrylamide concentration of solutions. The method comprises following steps: a three-electrode system, and differential pulse voltammetry are adopted to detect acrylamide in a solution to be detected, and the concentration of acrylamide is obtained based on an acrylamide linear equation, wherein a working electrode of the three-electrode system is a glassy carbon electrode modified with carboxylated grapheme and a characteristic single-stranded DNA, and the sequence of the characteristic single-stranded DNA is 5'-AAAAAAAAAGGAAAAAAAAA-(CH2)6-SH-3'. According to the method, differential pulse voltammetry is adopted for high sensitive detection on acrylamide; detection limit on acrylamide concentration can be as low as 1mol / L; operation is simple; detection is rapid; sensitivity and selectivity are high; and application prospect is promising.

Description

technical field [0001] The invention belongs to the technical field of chemical detection, in particular to a method for detecting the concentration of acrylamide in a solution. Background technique [0002] Acrylamide has mutagenic effects in both in vivo and in vitro tests, and can cause gene mutations and chromosomal abnormalities in mammalian somatic and germ cells, such as micronucleus formation, sister chromatid exchange, polyploidy, aneuploidy, and others Abnormal mitosis, etc., dominant lethal test was positive. It was also proved that the metabolite glycidamide of acrylamide is its main mutagenic active substance. Animal experiments have found that acrylamide can cause tumors in various organs of rats, including mammary gland, thyroid gland, testis, adrenal gland, central nervous system, oral cavity, uterus, and pituitary gland. The International Agency for Research on Cancer (IARC) evaluated its carcinogenicity in 1994, and listed acrylamide as a type 2 carcinoge...

Claims

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Application Information

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IPC IPC(8): G01N27/48G01N27/30
Inventor 肖琦黄珊卢双燕盛家荣黄初升
Owner NINGBO XIANAN CHEM
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