The invention provides an SPR measuring method and a measuring
system thereof. According to the measuring method, first, a linear-polarized coherent
broadband pulsed
light beam is incident onto a surface of a sample under detection of a SPR sensing device to cause reflection; next, a time-domain monotonous
chirp is performed on the incident coherent
broadband pulsed light and / or the SPR reflected light; finally, the time-domain monotonically chirped SPR reflected
light beam is detected, and information of the SPR effect is obtained according to the detected
signal. According to the invention, the spectrum detection method used in conventional slow
wavelength interrogation is converted into the high-speed real-time detection of time-domain pulse shape signals. Thus, the procedure of the SPR reaction may be monitored closely, and dynamical curves with very high
time resolution may be obtained, in that case, fast biochemical reaction procedures may be monitored. As each part of the measuring
system may remain fixed during the measurement, a compact, miniature and portable
system may be realized. The interrogation part of the
sensing system is easy-to-realize, of low cost and compatible with various SPR devices and components.