The invention relates to a zero distance calibration system and method for pulse Doppler radar. The calibration system comprises a circulator, a limiter, a low noise amplifier, isolators, a mixer, a delay line, an up-converter, a first amplifier, a second amplifier, a power divider and an antenna, wherein the circulator, the limiter, the first isolator, the low noise amplifier, the second isolator and the mixer which are sequentially connected in series constitute a reception channel; the delay line and impedance matching networks in front of and behind the delay line constitute a delay channel; the up-converter, the third isolator, the first amplifier, the fourth isolator, the second amplifier, the circulator and the antenna which are sequentially connected constitute a transmission channel; the delay channel is connected between the transmission channel and the reception channel, and electrical signals are converted into sound signals to be subjected to time delay propagation in sound transmission media.