Preparation and application of HP-beta-CD (hydroxypropyl beta-cyclodextrin) functionalized GO (graphene oxide) composite material

A composite material, hydroxypropyl technology, applied in the field of electrochemical applications, composite material preparation, can solve the problems of no curative effect, toxic and side effects, etc.

Inactive Publication Date: 2017-12-15
NORTHWEST NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

More than 80% of synthetic drugs are racemates, and usually only one has a therape

Method used

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  • Preparation and application of HP-beta-CD (hydroxypropyl beta-cyclodextrin) functionalized GO (graphene oxide) composite material
  • Preparation and application of HP-beta-CD (hydroxypropyl beta-cyclodextrin) functionalized GO (graphene oxide) composite material
  • Preparation and application of HP-beta-CD (hydroxypropyl beta-cyclodextrin) functionalized GO (graphene oxide) composite material

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] Example 1. Preparation of rGO-HP-β-CD chiral composite material

[0034] (1) Preparation of hydroxypropyl β-cyclodextrin

[0035] First, add 1g of β-CD to KOH (20wt%) solution, stir and react at room temperature for 6 hours, in order to fully activate β-CD, put it in the refrigerator for 22 hours, then add 3ml of propylene oxide, and stir at room temperature for 40~ 48 hours. Then use 10mM / L hydrochloric acid to adjust the pH of the mixed solution to 7, rotary steam at 45~50°C, then dissolve the product with 20ml of ethanol, filter with suction, add 200ml of acetone to the filtrate, stir quickly, filter with suction, wash, And vacuum-dried at 50-60°C for 60-72 hours to obtain white HP-β-CD powder, HP-β-CD has better water solubility than β-CD.

[0036] (2) Preparation of graphene oxide (GO)

[0037] Using the modified Hummers method, 1.5g flake graphite was placed in 20ml concentrated sulfuric acid dissolved with 2.5g potassium persulfate and 2.5g phosphorus pentoxid...

Embodiment 2

[0040] Embodiment 2, the preparation of chiral electrochemical sensor

[0041] The bare glassy carbon electrode (GCE) was coated with 0.3 μm, 0.05 μm and 0.02 μm Al on the suede 2 o 3 For polishing, first rinse the surface dirt with ultrapure water, then transfer to ethanol and ultrapure water for ultrasonication for 5~6min respectively. 5 mL of rGO-HP-β-CD with a mass concentration of 30 μg / ml was uniformly drop-coated on the surface-polished GCE to prepare a rGO-HP-β-CD / GCE chiral electrochemical sensor, and then rGO-HP-β- CD / GCE were placed in 5.0~6.0mM [Fe(CN) 6 ] 4- / 3- The solution (the solution contains 0.1~0.15M KCl) was tested by cyclic voltammetry (CV), and the peak current of the CV curve was 0.033mA.

Embodiment 3

[0042] Example 3. Recognition of Tryptophan Enantiomers by Chiral Electrochemical Sensors

[0043] The chiral electrochemical sensor rGO-HP-β-CD / GCE was respectively placed in the L-tryptophan or D-tryptophan solution with a concentration of 8-10mM / L and a volume of 20-25mL (the solution contained 0.2 ~0.3M PBS (pH=6.0) buffer solution and 0.5mM [Fe(CN) 6 ] 4- / 3- ), using differential pulse voltammetry (DPV) for chiral recognition of tryptophan enantiomers, scanning potential from 0.3 to 1.4V, scanning speed 100mV s -1 . When L-tryptophan and D-tryptophan interact with rGO-HP-β-CD / GCE, the peak current values ​​are different, so that the purpose of identifying the enantiomers of tryptophan can be achieved. When rGO-HP-β-CD / GCE interacted with L-tryptophan, the peak current was D-tryptophan.

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Abstract

The invention discloses preparation and application of HP-beta-CD (hydroxypropyl beta-cyclodextrin) functionalized GO (graphene oxide) chiral composite material, and belongs to the fields of material preparation and electrochemical application. The preparation comprises the following steps of firstly, enabling beta-CD and propylene oxide to react under the alkaline condition, so as to prepare the HP-beta-CD; then, performing in-situ reduction on the GO and the HP-beta-CD by hydrazine hydrate, so as to obtain the HP-beta-CD functionalized GO (rGO-HP-beta-CD) composite material; finally, dripping and spraying the rGO-HP-beta-CD composite material to the surface of a GCE (glassy carbon electrode), so as to obtain a rGO-HP-beta-CD/GCE chiral electrochemical sensor. The preparation has the advantage that the tryptophan enantiomer is chirally identified by a DPV (differential pulse voltammetry) method, so that the stronger identifying ability on the D-tryptophan by the chiral electrochemical sensor is found.

Description

technical field [0001] The present invention relates to the preparation of a hydroxypropyl β-cyclodextrin functionalized graphene oxide chiral composite material, and the present invention also relates to the chiral composite material used as a chiral recognition reagent in identifying tryptophan enantiomers The application of the body belongs to the field of composite material preparation and the field of electrochemical application. Background technique [0002] Chirality is ubiquitous in nature and organisms, and it plays an indispensable role in biological metabolism. Most molecules in living organisms are chiral, such as amino acids, polysaccharides, and nucleic acids. More than 80% of synthetic drugs are racemates, and usually only one has a therapeutic effect, while the other has no curative effect or even has toxic side effects. The study of chiral recognition plays an important guiding role in the detection of chiral drugs. Electrochemical sensors use racemate mo...

Claims

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Application Information

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IPC IPC(8): C08L5/16C08K3/04C08B37/16G01N27/49
CPCC08K3/04C08B37/0012G01N27/49C08L5/16
Inventor 莫尊理王瑞娟李振亮牛小惠郭瑞斌燕敏张红娟冯航空
Owner NORTHWEST NORMAL UNIVERSITY
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