Method for detecting concentration of acrylamide in solution

Abstract

The invention provides a method for detecting the concentration of acrylamide in a solution. The method comprises the steps of detecting acrylamide in a sample solution by using a three-electrode system and a differential pulse voltammetry; and obtaining the concentration of acrylamide in the sample solution according to a differential pulse voltammetry curve of acrylamide, wherein a working electrode in the three-electrode system is an aminated graphene and single-chain DNA modified electrode. The invention also provides concrete steps for detecting the concentration of acrylamide in the solution and a preparation method of the working electrode. The content of acrylamide in the solution can be rapidly and quantitatively detected, and the detection limit of acrylamide can be up to 5.1*10<-8>mol / L.

Description

technical field

[0001] The invention belongs to the technical field of chemical detection, and in particular relates to a method for detecting the concentration of acrylamide in a solution. Background technique

[0002] Acrylamide has mutagenic effects in both in vivo and in vitro tests, and can cause gene mutations and chromosomal abnormalities in mammalian somatic and germ cells, such as micronucleus formation, sister chromatid exchange, polyploidy, aneuploidy, and others Abnormal mitosis, etc., dominant lethal test was positive. It was also proved that the metabolite glycidamide of acrylamide is its main mutagenic active substance. Animal experiments have found that acrylamide can cause tumors in various organs of rats, including mammary gland, thyroid gland, testis, adrenal gland, central nervous system, oral cavity, uterus, and pituitary gland. The International Agency for Research on Cancer (IARC) evaluated its carcinogenicity in 1994, and listed acrylamide as a type...

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