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Electrochemical detection method for capsaicine in peppers

A detection method and electrochemical technology, applied in the field of electrochemical detection, can solve the problems of complicated operation steps, long detection time and high detection cost, and achieve the effects of reducing background current, simple operation and simple manufacturing process.

Active Publication Date: 2019-01-22
重庆医科大学国际体外诊断研究院
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The purpose of the present invention is to provide a simple and fast electrochemical detection method of capsaicin, which can be used for the detection of capsaicin in pepper samples, and solves the problems of low sensitivity, high detection cost, long detection time, etc. in the prior art. Complicated operation steps and other issues

Method used

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  • Electrochemical detection method for capsaicine in peppers
  • Electrochemical detection method for capsaicine in peppers
  • Electrochemical detection method for capsaicine in peppers

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] Example 1: Preparation of the screen-printed carbon electrode of the present invention

[0038] The screen printing carbon electrode of the present invention is to sequentially print carbon paste, Ag / AgCl paste and insulating paste on a PET substrate. Specifically include the following steps:

[0039] 1) Clean the PET substrate, print carbon paste on the PET substrate after drying, make a carbon working electrode and a carbon paste auxiliary electrode, and dry at room temperature;

[0040] 2) Print a silver paste containing silver chloride on the above PET substrate to make an Ag / AgCl reference electrode, and dry it at room temperature;

[0041] 3) Avoid the circular working area, and print insulating paste on the above PET substrate to cover the wires;

[0042] 4) The above-mentioned working electrode, auxiliary electrode and reference electrode form a circular working area, and each electrode is connected to the interface through a wire under the insulating film, and then drie...

Embodiment 2

[0044] Example 2: Electrochemical detection method of capsaicin in pepper

[0045] 1) Preparation of solution: prepare a 100.0mmol / L, pH 1.0 HCl solution with ultrapure water and place it in a container for later use.

[0046] 2) Sample pretreatment: Grind and homogenize fresh peppers, accurately weigh 0.2g into 12mL of absolute ethanol, stir and mix well.

[0047] 3) Place a volumetric flask containing the 50 mL mixture processed in step 2) in an ultrasonic cleaner, and stir for 2 hours with a magnetic stirrer after 20 minutes of ultrasonic treatment to thoroughly mix the capsicum with absolute ethanol.

[0048] 4) Centrifuge the solution obtained in step 3) at 10000 rpm and 4°C for 10 minutes, and save the supernatant. Filter the supernatant with a 0.22μm filter membrane, vortex and mix the filtrate, take 200μL into an Ep tube, and place it in a vacuum centrifugal concentrator for drying.

[0049] 5) Use 4 mL of the dried extract from step 4) containing HCl and supporting electrolyte...

Embodiment 3

[0052] This example is a verification of the detection principle of the electrochemical method, and the results are shown in figure 2 . In the figure, curve a is the DPV curve of the blank solution; curve b is the DPV curve of the capsaicin solution containing 10.0μmol / L without enrichment treatment; curve c is the DPV curve after stirring and enrichment at -0.1V enrichment potential for 60s DPV curve of capsaicin solution containing 10.0μmol / L. by figure 2 It can be seen that no obvious electrochemical response signal is observed in curve a; an oxidation peak current signal is generated at 0.39V in curve b, indicating that the obtained electrochemical signal is produced by oxidation of capsaicin. Comparing curves b and c, it can be seen that the electrochemical response signal of capsaicin is significantly improved after the enrichment treatment, indicating that the enrichment treatment effectively improves the detection sensitivity.

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Abstract

The invention discloses a simple and quick electrochemical capsaicine detection method. According to the method, simple, quick and correct quantitative detection for capsaicine is realized according to a linear relationship between electrochemical response signals and concentrations of to-be-detected objects by adoption of a differential pulse-adsorptive stripping voltammetry method through takinga non-modified silk-screen printing carbon electrode as an electrode material and combining the advantages that an adsorptive stripping voltammetry method is capable of greatly improving the detection sensitivity and a differential pulse voltammetry method is capable of effectively reducing the background current. The method is independent of large-scale instrument equipment; the used non-modified silk-screen printing carbon electrode is simple to prepare and low in cost; and the detection method is high in capsaicine detection sensitivity, simple and quick, can be used for detecting capsaicine in pepper samples, and is hopeful for being popularized and applied in the detection analysis and medicinal value research aspects of capsaicine.

Description

Technical field [0001] The invention belongs to the field of electrochemical detection, and mainly relates to a simple and rapid electrochemical detection method of capsaicin in capsicum. Background technique [0002] Capsaicin (also known as capsaicin) is an active ingredient obtained from plants of the genus Capsicum. It is a lipophilic, colorless and tasteless alkaloid. Capsaicin in peppers mainly includes capsaicin, dihydrocapsaicin, nordihydrocapsaicin, percapsaicin, and perhydrocapsaicin. Among them, capsaicin and dihydrocapsaicin account for about 90% of the total capsaicin, and also provide about 90% of the spicy and hot sensation. Capsaicin is derived from the pure natural plant pepper. It is not only widely used in the food industry as a spicing agent, but also because of its various pharmacological and physiological activities such as analgesia, anti-inflammatory, antipruritic, anti-oxidant, and antibacterial. In the pharmaceutical industry. In addition, capsaicin i...

Claims

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Application Information

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IPC IPC(8): G01N27/26G01N27/30
CPCG01N27/26G01N27/30
Inventor 张晓清周莹丁敏吕文静陈霄韩润川
Owner 重庆医科大学国际体外诊断研究院
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