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Electrochemical sensor for adapters and method for detecting chlorpyrifos

An aptamer and electrochemical technology, applied in the field of biochemistry, can solve the problems of difficult regeneration, poor selectivity, and high detection limit of sensing signals of biosensors, and achieve low cost, simple method, excellent biocompatibility and electrical conductivity. performance effect

Inactive Publication Date: 2017-11-21
CHONGQING UNIV
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Problems solved by technology

[0006] In view of the above-mentioned deficiencies in the prior art, the purpose of the present invention is to provide an aptamer electrochemical sensor and its preparation method, which can solve the problems of high detection limit, poor selectivity and difficult regeneration of the sensing signal of the biosensor.

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  • Electrochemical sensor for adapters and method for detecting chlorpyrifos
  • Electrochemical sensor for adapters and method for detecting chlorpyrifos
  • Electrochemical sensor for adapters and method for detecting chlorpyrifos

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Embodiment Construction

[0036] The present invention will be described in further detail below in conjunction with specific embodiments and accompanying drawings. The reagents described in the present invention are commercially available unless otherwise specified.

[0037] 1. An aptamer electrochemical sensor adopts a three-electrode system with a glassy carbon electrode as a substrate. The three-electrode system uses a biosensor as a working electrode, Ag / AgCl and 3M KCl as a reference electrode, and a platinum wire electrode as a Auxiliary electrode; the surface of the base body includes a first detection layer, a second detection layer and a fixed layer in sequence from outside to inside; the first detection layer includes an amino-modified capture probe of an aptamer and a part of its complementary chain, so The second detection layer includes copper oxide nanoflowers and single-wall carbon nanotubes, the fixed layer is nafion, and the first detection layer and the second detection layer are deh...

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Abstract

The invention discloses an electrochemical sensor for adapters and a method for detecting chlorpyrifos. The electrochemical sensor adopts a three-electrode system and comprises a first detection layer, a second detection layer and a fixed layer. A preparation method comprises the following steps of: preparing copper oxide nanoflower solution, preparing single-wall carbon nanotube solution, treating base electrodes, activating carboxyl groups, and fixing probes and the adapters; and then manufacturing a standard curve by utilizing a differential pulse voltammetry, and finally calculating out the concentration of the chlorpyrifos in samples according to the standard curve of the chlorpyrifos. The electrochemical sensor and the method disclosed by the invention have the advantages that the detection for the chlorpyrifos is specific, the sensitivity and the selectivity are high, the renewability is achieved, the materials are wide in source, the cost is low, and the preparation method is simple, so that the application prospect is good.

Description

technical field [0001] The invention relates to an aptamer electrochemical sensor for detecting residual pesticides and a preparation method, in particular to an aptamer electrochemical sensor and a preparation method, and a method for detecting chlorpyrifos. It belongs to the technical field of biochemistry. Background technique [0002] Organophosphorus pesticides (OPs) are among the most commonly used pesticides in the world, especially in developing countries, and can be converted into neurotoxins used in chemical weapons. Due to its inhibition of acetylcholinesterase (AChE) or cholinesterase (ChE) activity in the nervous system, OP can disrupt normal nerve impulse conduction, causing a series of toxic symptoms such as abnormal excitement, convulsions, paralysis and death. Therefore, OP is attracting more and more countries to set stricter standards for the safety application of OP. Chlorpyrifos (O,O-diethyl-O-(3,5,6-trichloro-2-pyridyl)phosphorothioate) is a seconda...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/327
CPCG01N27/3278G01N27/3277
Inventor 胥国立霍丹群侯长军包静杨眉
Owner CHONGQING UNIV
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