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Polybromocresol green modified glassy carbon electrode and application thereof

A glassy carbon electrode, polybromocresol technology, applied in the direction of electrochemical variables of materials, etc., to achieve the effect of improving sensitivity, increasing current response, good reproducibility and stability

Inactive Publication Date: 2015-05-13
SHANGHAI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The simultaneous detection of UA, DA and AA using electropolymerized bromocresol green modified glassy carbon electrodes has not been reported yet

Method used

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  • Polybromocresol green modified glassy carbon electrode and application thereof
  • Polybromocresol green modified glassy carbon electrode and application thereof
  • Polybromocresol green modified glassy carbon electrode and application thereof

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Embodiment 1

[0022] The preparation method and steps of the modified carbon paste electrode in the present embodiment are as follows:

[0023] (1) Pretreatment of the glassy carbon electrode: first, the glassy carbon electrode is treated with 0.05 μm Al 2 o 3 The polishing powder and suede are polished to a mirror surface, and then ultrasonically cleaned with distilled water, dilute nitric acid solution, absolute ethanol and double distilled water in sequence, and then used.

[0024] (2) A use and method of use as an electrochemical sensor for uric acid, dopamine and ascorbic acid in the process of detecting the concentration of uric acid, dopamine and ascorbic acid, the use of which is: the modified glassy carbon electrode can be directly used for the detection of uric acid, dopamine and ascorbic acid Simultaneous electrochemical determination; its use method and determination method are as follows: the glassy carbon electrode modified by electropolymerization bromocresol green is used a...

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Abstract

The invention relates to a polybromocresol green modified glassy carbon electrode and application thereof, and mainly relates to the preparation of electro-polymerized bromocresol green modified glassy carbon electrode, and the application of the electrode in the sensitive quantitative analysis determination of uric acid, dopamine and ascorbic acid through the differential pulse voltammetry. The experimental result shows that in a phosphate buffered solution with the concentration of 0.1 mol / L and the pH of 6, the modified electrode has obvious catalysis function and sensibilization on uric acid, dopamine and ascorbic acid, and under the optimum condition, and through the adoption of the differential pulse voltammetry, the concentrations of uric acid, dopamine and ascorbic acid have better linear relations with the peak current thereof within the ranges of 0.5-15 micrometers, 0.08-4.0 micrometers and 5-150 micrometers, and the detection limits reach 0.17, 0.017 and 0.17 micrometer respectively; the method shows satisfactory results when the electrode is applied to determination of contents of uric acid, dopamine and ascorbic acid in plasma.

Description

technical field [0001] The invention relates to the preparation and application of a glassy carbon electrode modified by polybromocresol green. Background technique [0002] Uric acid (UA) is the final oxidation product of purine metabolism in the human body and exists in human blood or urine. Normal levels of UA excreted in human serum and urine are in the range of 240-520 microns and 1.4-4.4 mm, respectively. High levels of uric acid can cause various diseases, such as gout and hyperuricemia. Dopamine (DA) is a major catecholamine neurotransmitter present in the mammalian brain. It controls a variety of functions in the human body, including motor behavior, cognition, emotion, food intake, and endocrine regulation, among others. Abnormalities in DA levels may cause neurological diseases such as Parkinson's disease and schizophrenia. L-Ascorbic acid (AA) is a soluble vitamin and a nutrient that is very important to humans. It plays an important role in the prevention a...

Claims

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Application Information

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IPC IPC(8): G01N27/30G01N27/48
Inventor 丁亚萍欧阳小茜罗立强李丽黄庵全
Owner SHANGHAI UNIV
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