GC-Orbitrap-MS screening and analyzing method for pesticide residues in animal-derived food

A technology of animal origin and analysis method, which is applied in the field of agricultural product quality and safety inspection, can solve the problems of large consumption of organic solvents, long time-consuming, cumbersome steps, etc.

Active Publication Date: 2021-02-02
INST OF QUALITY STANDARD & TESTING TECH FOR AGRO PROD OF CAAS
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] The matrix of animal-derived food is complex, and traditional pretreatment methods o

Method used

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  • GC-Orbitrap-MS screening and analyzing method for pesticide residues in animal-derived food
  • GC-Orbitrap-MS screening and analyzing method for pesticide residues in animal-derived food
  • GC-Orbitrap-MS screening and analyzing method for pesticide residues in animal-derived food

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Experimental program
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Embodiment 1

[0049] 1. Instrument working conditions:

[0050] Chromatographic conditions:

[0051]The chromatographic conditions of the gas chromatography are as follows: the heating program is: initial temperature 40°C, keep 1.5min; 25°C / min heat up to 90°C, keep 1.5min; 25°C / min heat up to 180°C; 5°C / min heat up to 280°C; 10°C / min to 300°C and keep for 3min. The inlet temperature was 290 °C, and the helium flow rate was 1.2 mL / min.

[0052] Mass Spectrometry Conditions:

[0053] The mass spectrometry conditions of the Orbitrap-MS method with an EI ion source are as follows: the ion source temperature is 280 °C, the ionization energy is 70 eV, and the current is 50 μA. The ion scan mode is full scan mode (Fullscan mode), the scan range is 50-550m / z, and the resolution is set to 60,000FWHM (200m / z).

[0054] The internal standard reference solution was used to calibrate the mass accuracy of the instrument in real time, and the mass spectrometry detection data was collected and process...

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Abstract

The invention discloses a GC-Orbitrap-MS screening and analyzing method for pesticide residues in animal-derived food. The method comprises the following steps: (1) extraction: homogenizing an animal-derived food sample, mixing the homogenized animal-derived food sample with an extraction solvent and an extraction salt, carrying out oscillation extraction to obtain an extraction solution, and taking a supernatant I; (2) purification: mixing the supernatant I obtained in the step (1) with a purifying agent for purification, collecting a supernatant II, and filtering to obtain a to-be-detected liquid sample; (3) chromatography-mass spectrometry detection: separating pesticide residues in the to-be-detected sample solution by adopting gas chromatography, and then detecting by adopting Orbitrap-MS with an EI ion source so as to obtain the content of the pesticide residues in the animal-derived food. The method disclosed by the invention has the characteristics of high recovery rate, good precision, strong applicability, small organic reagent dosage and the like; various technical indexes can meet the requirements of daily detection and analysis of various animal-derived foods, and important technical support is provided for the field of food safety.

Description

technical field [0001] The invention relates to a GC-Orbitrap-MS screening analysis method for pesticide residues in animal-derived food, belonging to the field of agricultural product quality and safety detection. Background technique [0002] Pesticides are indispensable auxiliary products in agricultural production. However, pesticides and their degradation products will accumulate in animals through the food chain, and then cause harm to human health. There are many types of pesticides, and their chemical properties vary greatly. Gas chromatography-orbitrap high-resolution mass spectrometry (GC-Orbitrap-MS) can provide accurate molecular mass and mass spectral fragmentation information of the compound to be tested, and has the advantages of high sensitivity, high accuracy and high resolution, and is increasingly used It is widely used in the analysis of pesticide residues in food. [0003] The matrix of animal-derived food is complex, and the traditional pretreatment m...

Claims

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Application Information

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IPC IPC(8): G01N30/02G01N30/72
CPCG01N30/02G01N30/72
Inventor 翁瑞庞旭邱静喻长远苏昕
Owner INST OF QUALITY STANDARD & TESTING TECH FOR AGRO PROD OF CAAS
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