LC-QToF-MS screening and analyzing method for pesticide residues in animal food

A technology for animal food and pesticide residues, which can be used in the analysis of materials, material separation, measurement devices, etc., and can solve the problems of long time, cumbersome steps, and different physical and chemical properties.

Active Publication Date: 2018-11-27
INST OF QUALITY STANDARD & TESTING TECH FOR AGRO PROD OF CAAS
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Compared with vegetables, fruits, etc., animal food products have more complex matrix components and different physical and chemical properties. Traditional extraction methods, such as Soxhlet extraction, solid-liquid extraction, and liquid-liquid extraction, often have cumbersome steps and consume a lot of time. It takes a

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  • LC-QToF-MS screening and analyzing method for pesticide residues in animal food
  • LC-QToF-MS screening and analyzing method for pesticide residues in animal food
  • LC-QToF-MS screening and analyzing method for pesticide residues in animal food

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[0054] Example 1,

[0055] 1. Instrument working conditions:

[0056] Chromatographic conditions:

[0057] Mobile phase A is an aqueous solution containing 5mmol / L ammonium formate and 0.1% formic acid; mobile phase B is an acetonitrile solution containing 5mmol / L ammonium formate and 0.1% formic acid; the gradient elution procedure is: 0min: 5% B, 0.5min: 5%B, 3.5min: 50%B, 17min: 100%B, 20min: 100%B, 20.1min: 5%B, run for 2min afterwards; flow rate is 0.4mL / min; column temperature: 35℃; sample volume : 2μL.

[0058] Mass spectrometry conditions:

[0059] The ion source spray voltage of the Agilent 6545 LC-QToF-MS mass spectrometer: 3500V; ion source temperature: 450℃; drying gas flow rate 6L / min; sheath flow gas flow rate 11L / min; sheath gas temperature 350℃; spray pressure 35psig; total The scanning mass-to-charge ratio ranges from 50 to 1700, and the internal standard reference solution is used to calibrate the mass accuracy of the instrument in real time. The mass spectrometry d...

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Abstract

The invention discloses an LC-QToF-MS screening and analyzing method for pesticide residues in animal food. The method comprises the following steps of: (1) extraction: homogenizing an animal food sample, mixing the animal food sample with an extraction solvent to obtain extract via vibration extraction, and carrying out dehydration and centrifugation on the extract to obtain liquid supernatant; (2) purification: mixing the liquid supernatant with a purification agent to carrying out purification, collecting the liquid supernatant, and carrying out thinning and filtration in sequence to obtainto-be-tested sample solution a; and (3) gas chromatgraphy-mass spectrometry detection: for the to-be-tested sample solution a, separating pesticide residue by adoption of a reversed phase liquid chromatography method, screening and analyzing the pesticide residue by adoption of LC-QToF-MS with an ESI ion source, and carrying out quantification by adoption of an external standard method so as to obtain a pesticide residue content in the animal food. The method is simple to operate, high in sensitivity and good in reproducibility and can be widely applied to screening and analysis of multiple pesticides in animal food.

Description

technical field [0001] The invention relates to an LC-QToF-MS screening analysis method for pesticide residues in animal foods, belonging to the fields of food hygiene and agricultural product quality and safety detection. Background technique [0002] Animal food not only faces the well-known risk of veterinary drug residue contamination, but may also be contaminated by pesticides through many channels. For example, herbicides, insecticides, and rodenticides used in the process of planting and storing feed crops may be transferred to animal food through the food chain; in the process of raising animals, acaricides may also be sprayed Cages are used to control ectoparasites, resulting in the residues of these pesticides in animal foods, and eventually lead to their accumulation in the human body through the enrichment of the food chain, threatening human health. Therefore, how to quickly, high-throughput, and high-precision screening and analysis of pesticide residues in an...

Claims

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Application Information

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IPC IPC(8): G01N30/89
CPCG01N30/89
Inventor 翁瑞邱静楼圣婷钱永忠宋月
Owner INST OF QUALITY STANDARD & TESTING TECH FOR AGRO PROD OF CAAS
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