LC-QToF-MS screening and analyzing method for pesticide residues in animal food
A technology for animal food and pesticide residues, which can be used in the analysis of materials, material separation, measurement devices, etc., and can solve the problems of long time, cumbersome steps, and different physical and chemical properties.
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[0055] 1. Instrument working conditions:
[0056] Chromatographic conditions:
[0057] Mobile phase A is an aqueous solution containing 5mmol / L ammonium formate and 0.1% formic acid; mobile phase B is an acetonitrile solution containing 5mmol / L ammonium formate and 0.1% formic acid; the gradient elution program is: 0min: 5% B, 0.5min: 5%B, 3.5min: 50%B, 17min: 100%B, 20min: 100%B, 20.1min: 5%B, last run for 2min; flow rate: 0.4mL / min; column temperature: 35℃; injection volume : 2 μL.
[0058] Mass Spectrometry Conditions:
[0059] The ion source spray voltage of Agilent 6545 LC-QToF-MS mass spectrometer: 3500V; ion source temperature: 450°C; drying gas flow rate 6L / min; sheath flow rate 11L / min; sheath flow gas temperature 350°C; The scanning mass-to-charge ratio ranges from 50 to 1700, and the internal standard reference solution is used to correct the mass accuracy of the instrument in real time, and the mass spectrometry data is collected and processed through AgilentMas...
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