Method for detecting fumonisin B1 based on fluorescence resonance energy transfer

A technology of fluorescence resonance energy and fumonisin, which is applied in the field of nanomaterials and analytical chemistry, can solve the problems of high cost, cumbersome operation, and high sample processing requirements, improve accuracy and stability, simplify pretreatment steps, shorten The effect of detection time

Active Publication Date: 2014-07-02
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Thanks to FB 1 It has the most content in natural food, so most of the current detection methods are aimed at FB 1 Such as: thin-layer chromatography TLC, high performance liquid chromatography HPLC, liquid chromatography-mass spectrometry, chromatography generally has good accuracy and repeatability, but the operation is relatively cumbersome and expensive, especially the sample processing requirements are high, not Suitable for mass sample testing
Enzyme-linked immunoassay (ELISA), due to its high specificity and sensitivity, the sample can be detected almost without special treatment, and it is suitable for the detection of large batches of samples, but the stability of the reagent is still difficult to meet the requirements

Method used

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  • Method for detecting fumonisin B1 based on fluorescence resonance energy transfer
  • Method for detecting fumonisin B1 based on fluorescence resonance energy transfer
  • Method for detecting fumonisin B1 based on fluorescence resonance energy transfer

Examples

Experimental program
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Effect test

Embodiment 1

[0027] Example 1: Fumonisin B in actual corn samples 1 Establishment of detection standard curve and detection sample pretreatment: crush and sieve corn at high speed, weigh 20g into a 100mL flask, add 5gNaCl, 80% ethanol aqueous solution, mix well, place in a homogenizer for high-speed stirring and extraction for 2min, and stand still for a while , filter, take 10mL of the filtrate and place it in a 50mL flask, stir and extract it again in a homogenizer at high speed for 2min, after resting, filter it with ultra-fine glass fiber filter paper until the filtrate is clear, collect the filtrate for later use.

[0028] Purchase 15 different types of corn from four local farmer's markets, and use the method of the present invention and enzyme-linked immunoassay to measure fumonisin B in them respectively 1 The results are shown in Table 1, and the obtained data were compared for correlation, the result was P1 detection.

[0029] Table 1: The actual sample detection of corn, the me...

Embodiment 2

[0032] Example 2: Fumonisin B in actual corn samples 1 The detection and standard addition recovery test sample pretreatment is the same as in Example 1.

[0033] 15 groups of fumonisins B obtained from Example 1 1 Select three groups from the concentration data as the background value, and then select three different concentrations of FB of 0.5ng / mL, 5.0ng / mL, and 50ng / mL 1 Standards are added to the analyte respectively, and the method of the present invention is used to detect again the FB 1 content to obtain the detection value. Recovery %=(detection value-background value) / addition amount×100%. From the results of Table 2, the rate of recovery is 91.1% to 120%, indicating that the present invention is stable, sensitive and accurate, and is suitable for FB in corn actual samples 1 detection.

[0034] Table 2: Fumonisin B in actual corn samples 1 Detection and spike recovery

[0035]

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Abstract

The present invention provides a method for detecting fumonisin B1 based on fluorescence resonance energy transfer. The method comprises that: NaYF4:Yb,Ho upconversion fluorescence nanoparticles and gold nanoparticles are respectively and covalently bound to both ends of the stem-like part of the specific molecular beacon, wherein the fluorescence resonance energy transfer upconversion fluorescence can be quenched by the gold nanoparticles; and a fumonisin B1 aptamer and a complementary strand thereof are subjected to hybridization immobilization on the surface of magnetic nanoparticles, an analyte fumonisin B1 is added and is specifically bound with the aptamer so as to make the complementary strand be separated from the magnetic nanoparticles, and the separated complementary strand and the molecular beacon are subjected to hybridization so as to open the ring-like part of the molecular beacon, such that the fluorescence resonance energy transfer system is broken, the fluorescence signal is restored, and the fumonisin B1 content can be determined by detecting the fluorescence signal intensity, wherein the detection limitation of the method achieves 0.01 ng.mL<-1>. According to the present invention, the fumonisin B1 content in the food sample can be rapidly, accurately e and sensitively detected.

Description

technical field [0001] A Fluorescence Resonance Energy Transfer-Based Detection of Fumonisin B 1 (FB 1 ) method, which belongs to the technical field of nanomaterials and analytical chemistry, and is used for FB in food and food 1 content was tested. Background technique [0002] Fumonisin (FB) is a class of mycotoxins mainly produced by Fusarium moniliforme. There are currently 11 known derivatives of FB, of which FB 1 (C 34 h 59 o 15 N, molecular weight 721) and FB 2 (C 34 h 59 o 14 N, molecular weight 705) is the main component of food contamination and the main cause of FB toxicity. Research has confirmed that FB 1 、FB 2 Can cause equine leukomalacia (ELEM) and porcine pulmonary edema (PPE). FB also poses a threat to human health. If infected, it may induce diseases such as esophageal cancer, liver cancer, and gastric cancer. Studies have shown that FB especially FB 1 Widely present in corn and its products; in rice, wheat, barley, condiments, sorghum, be...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/64
Inventor 王周平吴世嘉段诺马小媛夏雨
Owner JIANGNAN UNIV
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