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36 results about "Simple Repetitive Sequences" patented technology
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Method for determining soybean phytophthora root rot resistant quantitative trait loci and use of the loci
InactiveCN101372710AReduce manpowerReduce material wasteMicrobiological testing/measurementPlant genotype modificationLoss ratePhytophthora sp.
The invention discloses a method for determining quantitative trait loci (QTL) for soybean phytophthora root rot tolerance and the application of the loci, comprising the following steps: carrying out analysis (genotype data analysis) upon the DNA of the descendant of the disease tolerant variety (female parent) and the infected strain (male parent) by utilizing random primer (RAPD), repeat sequence primer (SSR) and sequence characteristic application range primer (SCAR); conducting inoculation identification upon the descendant with home and aboard mixed strain and having statistics upon the disease loss rate (phenotype data analysis); calculating the existent threshold of quantitative trait loci (QTL) for soybean phytophthora root rot tolerance with regression algorithm and detecting whether there are quantitative trait loci (QTL) for soybean phytophthora root rot tolerance with WinQTLCarter2.0; and finally judging whether the genealogies or derived varieties have the quantitative trait loci (QTL) by detecting the DNA of parent and genealogies or derived varieties thereof. The invention can detect whether the genealogies or derived varieties have disease tolerance so as to speed up the seed selection for varieties (strains) of soybean phytophthora root rot tolerance.
Owner:李 文滨
Method for identifying cymbidium varieties
InactiveCN101818199AResolve genetic relationshipsSolve problems such as authenticity checksMicrobiological testing/measurementMolecular identificationInformation diversity
The invention provides a molecular identification method for cymbidium varieties, which is a simple sequence repeat genome analysis-based molecular identification method for the cymbidium varieties and analyzes the genetic relation of the cymbidium varieties by optimizing a technical system of cymbidium simple sequence repeat (SSR) analysis. By directly detecting the DNA sequence difference of the variety with genetic stability and information diversity, the method can definitely distinguish and judge different varieties of cymbidium, and realize accurate and quick identification of the variety so as to provide a reliable technique for variety identification, seed and seedling market management, variety protection and elite seed breeding and the like. By optimizing the SSR technical system of cymbidium genome DNA, the molecular identification of the cymbidium varieties has high speed, short time and multiple polymorphisms, the problems of reality inspection and the like of the cymbidium seedlings can be solved before trading, and the loss of a producer or a buyer is avoided. The method can distinguish and identify all cymbidium varieties by using one and more effective primers, and realize DNA fingerprint identification of the cymbidium varieties.
Owner:ZHEJIANG UNIV
Method for detecting redried tobacco varietal complexity
InactiveCN105256065AAvoid blind selectionImprove screening efficiencyMicrobiological testing/measurementSpectral bandsComparison standard
The invention relates to a method for detecting redried tobacco varietal complexity. According to the technical scheme, the method comprises the following steps of 1, extraction of redried tobacoo DNA; 2, synthesis of primers; 3, a simple repetition inter-sequence polymorphism-polymerization enzyme-linked reaction, wherein the ISSR primer 826 or the ISSR primer 856 is subjected to an ISSR-PCR in redried tobacco to be detected; 4, comparison of a standard diagram, wherein strip comparison is performed on a standard fingerprint chromatogram and an imaging picture obtained after electrophoresis of the redried tobacco ISSR-PCR to be detected is performed, if strips can be matched, the refried tobacco is in one corresponding variety, and if the strips cannot be matched, the refried tobacco is in the mixed variety, and detection is completed. The method has the advantages that the method is provided for authentication and detection of redried tobacco, the primers obtained through screening of the experiment can be directly referred to in the redried tobacco experiment process and subjected to an ISSR-PCR directly at the corresponding annealing temperature, and then the fingerprint spectrum in the corresponding variety is obtained to be used as a follow-up comparison standard spectral band.
Owner:SOUTHWEST FORESTRY UNIVERSITY
Primer pair, castanopsis hystrix SSR7 (Simple Sequence Repeat 7) marker and preparation method and application thereof
InactiveCN103740706AGood repeatabilityImprove reliabilityMicrobiological testing/measurementDNA preparationGenetic diversityCastanopsis hystrix
The invention discloses a primer pair, a castanopsis hystrix SSR7 (Simple Sequence Repeat 7) marker and a preparation method and application thereof. According to the preparation method, the total DNA (Deoxyribonucleic Acid) of castanopsis hystrix is subjected to PCR (Polymerase Chain Reaction) amplification by using the specific primer pair HZ7, thereby obtaining the SSR7 marker. The method is simple and feasible and is simple and convenient in operation; shown by tests which are carried out in members of two natural castanopsis hystrix populations and among the members, the obtained SSR has relatively high polymorphism. The castanopsis hystrix SSR7 marker is a co-dominant marker; compared with other molecular markers, the SSR marker is good in repeatability and high in reliability, can be applied to the genetic diversity evaluation of castanopsis hystrix, the genetic map construction of castanopsis hystrix, the research on the spatial distribution pattern of the genetic variation of castanopsis hystrix population and the origination and evolution of castanopsis hystrix and the standardization of target genes, and can also be applied to the molecular marker assisted breeding and QTL (Quantitative Trait Locus) research of castanopsis hystrix.
Owner:GUANGXI FORESTRY RES INST
Zelkova SSR1 marker and primer pair and preparation methods and application thereof
InactiveCN104450693AGood repeatabilityImprove reliabilityMicrobiological testing/measurementDNA/RNA fragmentationGenetic diversitySimple Repetitive Sequences
The invention discloses a Zelkova SSR1 marker and a primer pair and preparation methods and applications thereof. PCR amplification is carried out on Zelkova total DNAs of JS1 by an inventor utilizing a specific primer, so that the SSR1 marker with a simple and repeated sequence is obtained. The method is simple and practicable and simple and convenient to operate, the obtained simple and repeated sequence shows higher polymorphism for detection inside or among populations of three Zelkova natural colonies. The Zelkova SSR1 marker is a co-dominant marker; compared with other molecular markers, the SSR marker is good in repeatability and high in reliability, can be applied to Zelkova genetic diversity evaluation, Zelkova genetic map construction, Zelkova population heritable variation spatial distribution pattern and Zelkova origin and evolution research as well as target gene marking, and can also be applied to Zelkova molecular marker assistant breeding and QTL (quantitative Trait Locus) research.
Owner:CENTRAL SOUTH UNIVERSITY OF FORESTRY AND TECHNOLOGY
Primer pair, castanopsis hystrix SSR5 (Simple Sequence Repeat 5) marker and preparation method and application thereof
InactiveCN103740703AGood repeatabilityImprove reliabilityMicrobiological testing/measurementDNA preparationGenetic diversityCastanopsis hystrix
The invention discloses a primer pair, a castanopsis hystrix SSR5 (Simple Sequence Repeat 5) marker and a preparation method and application thereof. According to the preparation method, the total DNA (Deoxyribonucleic Acid) of castanopsis hystrix is subjected to PCR (Polymerase Chain Reaction) amplification by using the specific primer pair HZ5, thereby obtaining the SSR5 marker. The method is simple and feasible and is simple and convenient in operation; shown by tests which are carried out in members of two natural castanopsis hystrix populations and among the members, the obtained SSR has relatively high polymorphism. The castanopsis hystrix SSR5 marker is a co-dominant marker; compared with other molecular markers, the SSR marker is good in repeatability and high in reliability, can be applied to the genetic diversity evaluation of castanopsis hystrix, the genetic map construction of castanopsis hystrix, the research on the spatial distribution pattern of the genetic variation of castanopsis hystrix population and the origination and evolution of castanopsis hystrix and the standardization of target genes, and can also be applied to the molecular marker assisted breeding and QTL (Quantitative Trait Locus) research of castanopsis hystrix.
Owner:GUANGXI FORESTRY RES INST
Primer pair, castanopsis hystrix SSR3 (Simple Sequence Repeat 3) marker and preparation method and application thereof
InactiveCN103740705AGood repeatabilityImprove reliabilityMicrobiological testing/measurementDNA preparationGenetic diversityCastanopsis hystrix
The invention discloses a primer pair, a castanopsis hystrix SSR3 (Simple Sequence Repeat 3) marker and a preparation method and application thereof. According to the preparation method, the total DNA (Deoxyribonucleic Acid) of castanopsis hystrix is subjected to PCR (Polymerase Chain Reaction) amplification by using the specific primer pair HZ3, thereby obtaining the SSR3 marker. The method is simple and feasible and is simple and convenient in operation; shown by tests which are carried out in members of two natural castanopsis hystrix populations and among the members, the obtained SSR has relatively high polymorphism. The castanopsis hystrix SSR3 marker is a co-dominant marker; compared with other molecular markers, the SSR marker is good in repeatability and high in reliability, can be applied to the genetic diversity evaluation of castanopsis hystrix, the genetic map construction of castanopsis hystrix, the research on the spatial distribution pattern of the genetic variation of castanopsis hystrix population and the origination and evolution of castanopsis hystrix and the standardization of target genes, and can also be applied to the molecular marker assisted breeding and QTL (Quantitative Trait Locus) research of castanopsis hystrix.
Owner:GUANGXI FORESTRY RES INST
Nucleotides sequence, molecule probe and method for identifying zhejiang fritillary variant-dong fritillary
InactiveCN101139605AReduce dosageHigh sensitivityMicrobiological testing/measurementGenetic engineeringFritillaria thunbergiiNucleotide sequencing
The invention discloses a nucleotide sequence, a nucleic acid molecular probe and a method for verifying a variant Fritillaria thunbergii.Miq.var. chekiangensis Hsiao et K.C.Hsia, and pertains to the technical field for verifying traditional Chinese medicine. The invention provides a special sequence for expanded ISSR between simple repeated sequences of Fritillaria thunbergii.Miq.var. chekiangensis Hsiao et K.C.Hsia and the verifying probe and method based on the sequence for the Fritillaria thunbergii.Miq.var. chekiangensis Hsiao et K.C.Hsia. The benefits with the invention are: 1) less sample use. The whole operation can be completed with few sample; 2) high accuracy and sensibility. The friD1 / friD2 is a special molecular probe for Fritillaria thunbergii.Miq.var. chekiangensis Hsiao et K.C.Hsia, and the probe will have negative reaction for other species.
Owner:ZHEJIANG UNIV
Simple repetitive sequence primer for portunus tritubereulatus
InactiveCN1908190AStrip clearGood repeatabilityMicrobiological testing/measurementFermentationBiologyPortunus trituberculatus
Owner:HUAIHAI INST OF TECH
Primer pair, castanopsis hystrix SSR4 (Simple Sequence Repeat 4) marker and preparation method and application thereof
InactiveCN103740704AGood repeatabilityImprove reliabilityMicrobiological testing/measurementDNA preparationGenetic diversityCastanopsis hystrix
The invention discloses a primer pair, a castanopsis hystrix SSR4 (Simple Sequence Repeat 4) marker and a preparation method and application thereof. According to the preparation method, the total DNA (Deoxyribonucleic Acid) of castanopsis hystrix is subjected to PCR (Polymerase Chain Reaction) amplification by using the specific primer pair HZ4, thereby obtaining the SSR4 marker. The method is simple and feasible and is simple and convenient in operation; shown by tests which are carried out in members of two natural castanopsis hystrix populations and among the members, the obtained SSR has relatively high polymorphism. The castanopsis hystrix SSR4 marker is a co-dominant marker; compared with other molecular markers, the SSR marker is good in repeatability and high in reliability, can be applied to the genetic diversity evaluation of castanopsis hystrix, the genetic map construction of castanopsis hystrix, the research on the spatial distribution pattern of the genetic variation of castanopsis hystrix population and the origination and evolution of castanopsis hystrix and the standardization of target genes, and can also be applied to the molecular marker assisted breeding and QTL (Quantitative Trait Locus) research of castanopsis hystrix.
Owner:GUANGXI FORESTRY RES INST
Primer pair, castanopsis hystrix SSR2 (Simple Sequence Repeat 2) marker and preparation method and application thereof
InactiveCN103740707ASimple and fast operationHigh polymorphismMicrobiological testing/measurementDNA preparationBiotechnologyGenetic diversity
The invention discloses a primer pair, a castanopsis hystrix SSR2 (Simple Sequence Repeat 2) marker and a preparation method and application thereof. According to the preparation method, the total DNA (Deoxyribonucleic Acid) of castanopsis hystrix is subjected to PCR (Polymerase Chain Reaction) amplification by using the specific primer pair HZ2, thereby obtaining the SSR2 marker. The method is simple and feasible and is simple and convenient in operation; shown by tests which are carried out in members of two natural castanopsis hystrix populations and among the members, the obtained SSR has relatively high polymorphism. The castanopsis hystrix SSR2 marker is a co-dominant marker; compared with other molecular markers, the SSR marker is good in repeatability and high in reliability, can be applied to the genetic diversity evaluation of castanopsis hystrix, the genetic map construction of castanopsis hystrix, the research on the spatial distribution pattern of the genetic variation of castanopsis hystrix population and the origination and evolution of castanopsis hystrix and the standardization of target genes, and can also be applied to the molecular marker assisted breeding and QTL (Quantitative Trait Locus) research of castanopsis hystrix.
Owner:GUANGXI FORESTRY RES INST
Capture probe set for detecting food allergens as well as preparation method and application of capture probe set
PendingCN113755555AGuaranteed capture efficiencyMicrobiological testing/measurementSequence analysisFood sensitizationSingle strand
The invention discloses a capture probe set for detecting food allergens as well as a preparation method and application of the capture probe set. The preparation method comprises the following steps of comparing gene segments of at least two allergens in pairs according to sequence similarity; classifying genes with the lengths of more than 120bp and the similarity of not less than 90% among allergen genes into one type; carrying out classification treatment to finally obtain at least one type of target genes, and carrying out multi-sequence comparison on the target genes by utilizing clustalw2 software; obtaining a consensus sequence of each type of target genes as a candidate probe by using a BioPerl module of a Perl language; and for each candidate probe, sequentially intercepting a sequence with the length of 100-130bp, and taking the probe which has the CG content of 30-70% and does not contain simple repetitive sequences as a single-stranded probe. The invention has the beneficial effects that high-throughput detection of sensitization components and sensitization pollutants in a complex food system can be realized, and the application value is very high.
Owner:ZHEJIANG GONGSHANG UNIVERSITY +1
A method for rapid identification of chromosomal ploidy in Avena plants and its application
InactiveCN104357577BRapid identificationMicrobiological testing/measurementDNA/RNA fragmentationRapid identificationPloidy
The invention belongs to the technical field of plant biotechnology, and specifically discloses a method for rapidly identifying chromosome ploidy of an avena plant. An SSR (Simple Sequence Repeats) molecule is utilized to mark characteristics that avena plants with different ploidy have different sizes and copy numbers, and the chromosome ploidy of the avena plants can be identified rapidly by combining a PCR amplification technology and a gel electrophoresis technology by virtue of different numbers of amplified product segments. The method for rapidly identifying chromosome ploidy of the avena plant has the advantage that ploidy of the plant can be identified rapidly under the condition that the ploidy of the avena plan is not known in advance, and the method is applicable to avena plants.
Owner:INST OF CROP SCI CHINESE ACAD OF AGRI SCI
Simple repetitive sequence primer for portunus tritubereulatus
InactiveCN100467614CStrip clearGood repeatabilityMicrobiological testing/measurementFermentationBiologyPortunus trituberculatus
Owner:HUAIHAI INST OF TECH
SSR (Simple Sequence Repeat) marker for simultaneously identifying sterile cytoplasm of gossypium harknessii and gossypium hirsutum
PendingCN113755636AEasy to distinguishImprove accuracyMicrobiological testing/measurementDNA/RNA fragmentationBiotechnologyNucleotide
The invention discloses an SSR (Simple Sequence Repeat) marker for simultaneously identifying sterile cytoplasm of gossypium harknessii and gossypium hirsutum. Primers of the SSR marker are shown as SEQ ID NO.1-2, and the SSR marker is not only linked with a gossypium harknessii cytoplasmic male sterility gene, but also linked with a gossypium hirsutum cytoplasmic male sterility gene. The simple repetitive sequence polymorphism of the nucleotide is presented on a polyacrylamide electrophoretogram, whether a single cotton plant contains a sterile gene or not can be identified according to an amplification band, and the type (CMS-D2 / CMS-D8) containing the sterile gene can be judged. By utilizing the same SSR marker, not only can the sterile line and the maintainer line of a cotton three-line material be identified, but also two sets of cytoplasmic male sterility genes of the cotton can be distinguished at the same time, and hybrid seeds matched with different three lines can be identified. The SSR marker plays an important role in breeding new cotton materials with different sterile genes and ensuring the purity of three-line hybrid seeds.
Owner:INST OF COTTON RES CHINESE ACAD OF AGRI SCI
Exploring method and exploring device for biological genome simple repeat sequence
InactiveCN102495977BReduce configuration complexityImprove the efficiency of excavationSpecial data processing applicationsGenomeRegular expression
The invention discloses an exploring method for a biological genome simple repeat sequence, which is characterized by comprising the following steps of: forming a regular expression according to the characteristics of the biological genome SSR (Simple Repeat Sequence) needed to be explored; analyzing a to-be-analyzed sequence according to the regular expression, judging whether the to-be-analyzed sequence contains a target SSR meeting the requirement of the regular expression, if so, outputting the target SSR; if not, displaying information about that the to-be-analyzed sequence contains no target SSR. Thus, the exploring method and the exploring device for the biological genome simple repeat sequence cannot generate redundant result in a SSR exploring process, so that the configuration complexity of the SSR exploring process is reduced, SSR exploring efficiency is improved and difficulty in development of SSR exploring software is reduced.
Owner:TOBACCO RES INST CHIN AGRI SCI ACAD
Red cone ssr1 marker, primer pair, preparation method and application thereof
InactiveCN103740708BGood repeatabilityImprove reliabilityMicrobiological testing/measurementDNA preparationGenetic diversityCastanopsis hystrix
The invention discloses a primer pair, a castanopsis hystrix SSR1 (Simple Sequence Repeat 1) marker and a preparation method and application thereof. According to the preparation method, the total DNA (Deoxyribonucleic Acid) of castanopsis hystrix is subjected to PCR (Polymerase Chain Reaction) amplification by using the specific primer pair HZ1, thereby obtaining the SSR1 marker. The method is simple and feasible and is simple and convenient in operation; shown by tests which are carried out in members of two natural castanopsis hystrix populations and among the members, the obtained SSR has relatively high polymorphism. The castanopsis hystrix SSR1 marker is a co-dominant marker; compared with other molecular markers, the SSR marker is good in repeatability and high in reliability, can be applied to the genetic diversity evaluation of castanopsis hystrix, the genetic map construction of castanopsis hystrix, the research on the spatial distribution pattern of the genetic variation of castanopsis hystrix population and the origination and evolution of castanopsis hystrix and the standardization of target genes, and can also be applied to the molecular marker assisted breeding and QTL (Quantitative Trait Locus) research of castanopsis hystrix.
Owner:GUANGXI FORESTRY RES INST
Hibiscus cannabinus L. drought response gene EST-SSR primers and kit
ActiveCN108048596AGood mark reserveMicrobiological testing/measurementDNA/RNA fragmentationHibiscusGenetic diversity
The invention discloses hibiscus cannabinus L. drought response gene EST-SSR primers and a kit. 11 pairs of primers are provided, and one pair or multiple pairs of primers are used as EST-SSR polymorphism markers for identifying the genetic relationship of hibiscus cannabinus L., the genetic diversity or the differences of simple repeat sequence polymorphisms in specific exon regions of drought-related genes. The hibiscus cannabinus L. drought response gene EST-SSR primers and the kit disclosed by the invention have the benefits that a good marker reserve is provided for molecular marker-assisted breeding of the hibiscus cannabinus L., and a candidate molecular marker for detection is also provided for functional allelic variation of key genes screened in the natural variation of the hibiscus cannabinus L.
Owner:ZHEJIANG XIAOSHAN COTTON & FLAX RES INST
Red cone ssr5 marker, primer pair, preparation method and application thereof
InactiveCN103740703BGood repeatabilityImprove reliabilityMicrobiological testing/measurementDNA preparationAgricultural scienceGenetic diversity
The invention discloses a primer pair, a castanopsis hystrix SSR5 (Simple Sequence Repeat 5) marker and a preparation method and application thereof. According to the preparation method, the total DNA (Deoxyribonucleic Acid) of castanopsis hystrix is subjected to PCR (Polymerase Chain Reaction) amplification by using the specific primer pair HZ5, thereby obtaining the SSR5 marker. The method is simple and feasible and is simple and convenient in operation; shown by tests which are carried out in members of two natural castanopsis hystrix populations and among the members, the obtained SSR has relatively high polymorphism. The castanopsis hystrix SSR5 marker is a co-dominant marker; compared with other molecular markers, the SSR marker is good in repeatability and high in reliability, can be applied to the genetic diversity evaluation of castanopsis hystrix, the genetic map construction of castanopsis hystrix, the research on the spatial distribution pattern of the genetic variation of castanopsis hystrix population and the origination and evolution of castanopsis hystrix and the standardization of target genes, and can also be applied to the molecular marker assisted breeding and QTL (Quantitative Trait Locus) research of castanopsis hystrix.
Owner:GUANGXI FORESTRY RES INST
Kenaf drought response gene est-ssr primer and kit
ActiveCN108048596BGood mark reserveMicrobiological testing/measurementDNA/RNA fragmentationBiotechnologyHibiscus
The invention discloses hibiscus cannabinus L. drought response gene EST-SSR primers and a kit. 11 pairs of primers are provided, and one pair or multiple pairs of primers are used as EST-SSR polymorphism markers for identifying the genetic relationship of hibiscus cannabinus L., the genetic diversity or the differences of simple repeat sequence polymorphisms in specific exon regions of drought-related genes. The hibiscus cannabinus L. drought response gene EST-SSR primers and the kit disclosed by the invention have the benefits that a good marker reserve is provided for molecular marker-assisted breeding of the hibiscus cannabinus L., and a candidate molecular marker for detection is also provided for functional allelic variation of key genes screened in the natural variation of the hibiscus cannabinus L.
Owner:ZHEJIANG XIAOSHAN COTTON & FLAX RES INST
Beech ssr1 marker, primer pair, preparation method and application thereof
InactiveCN104450693BGood repeatabilityImprove reliabilityMicrobiological testing/measurementDNA/RNA fragmentationGenetic diversityBeech
The invention discloses a Beech tree SSR1 marker, a primer pair and a preparation method and application thereof. The inventor uses a specific primer pair JS1 to amplify the total DNA of the beech tree by PCR to obtain a simple repeat sequence SSR1 marker. The method is simple and easy to operate, and the obtained simple repeat sequences have high polymorphisms in the population and between populations of the three natural populations of Zelkova. Beech tree SSR1 marker is a co-dominant marker. Compared with other molecular markers, SSR marker has good repeatability and high reliability. It can be applied to the evaluation of genetic diversity of beech tree, the construction of genetic map of beech tree, and the spatial distribution of genetic variation of beech tree population. The study of pattern and origin and evolution of Beech trees, as well as the calibration of target genes, can also be applied to molecular marker-assisted breeding and QTL research of Beech trees.
Owner:CENTRAL SOUTH UNIVERSITY OF FORESTRY AND TECHNOLOGY
Beech ssr2 marker, primer pair, preparation method and application thereof
InactiveCN104450692BGood repeatabilityImprove reliabilityMicrobiological testing/measurementDNA preparationGenetic diversityBeech
The invention discloses a beech SSR2 marker, a primer pair, its preparation method and application. The inventor uses a specific primer pair JS2 to perform PCR amplification on the beech total DNA to obtain a simple repeat sequence SSR2 marker. The method is simple and easy to operate, and the obtained simple repeat sequence has high polymorphism detected in the population and between populations of three natural populations of beech. The beech SSR2 marker is a co-dominant marker. Compared with other molecular markers, the SSR marker has good repeatability and high reliability. It can be applied to the evaluation of genetic diversity of beech, the construction of beech genetic map, and the spatial distribution of genetic variation in beech populations. The research on pattern and origin and evolution of beech, as well as the calibration of target genes, can also be applied to molecular marker-assisted breeding and QTL research of beech.
Owner:CENTRAL SOUTH UNIVERSITY OF FORESTRY AND TECHNOLOGY
A high-throughput, versatile typing technique for multiple types of molecular markers
ActiveCN104830993BImprove throughputLow costMicrobiological testing/measurementInsertion deletionSolution hybridization
The invention discloses a high-throughput typing technique universal to various molecular markers and belongs to the field of molecular biology. By the use of hybrid template production of 5' terminal biotin labeling, a target area having single-nucleotide polymorphism, simple sequence repeat, insertion deletion, copy number variation and the like and containing various molecular markers and target areas such as exon and species specific single-copy areas can be subjected to capturing and gene typing; meanwhile, allele quantitative typing and precise quantitative detecting are both considered; site selection flexibility of the solution hybridization is combined with the advantages of the high-throughput sequencing technique, such as high throughput and low cost. Compared with the use of the existing molecular marker typing technique, the use of the high-throughput typing technique solves the problems such that the existing fixed-point molecular marker typing technique is available for only typing single molecular markers and unavailable for typing various markers at the same time or precisely and quantitatively detecting alleles, and production of random markers or unlabeled hybrid templates is limited.
Owner:OCEAN UNIV OF CHINA
Simple repetition sequence primer for moerella iridescens
InactiveCN101429558BStrip clearGood repeatabilityMicrobiological testing/measurementDNA/RNA fragmentationRainbowAgricultural science
The invention relates to a simple repetitive sequence primer for Rainbow Moerella, which is characterized in that the simple repetitive sequence primer for the Rainbow Moerella is a primer group which is formed by 10 Rainbow Moerella primers. The screened amplified products of the primer are clear and stable, have good polymorphism, can be applied in idioplasm evaluation and idioplasm identification of the Rainbow Moerella, and diversity evaluation of colonies and remains of the Rainbow Moerella, and have the characteristics of quickness, economy, accuracy, simple and convenient operation andso on.
Owner:HUAIHAI INST OF TECH +1
Red cone ssr2 marker, primer pair, preparation method and application thereof
InactiveCN103740707BGood repeatabilityImprove reliabilityMicrobiological testing/measurementDNA preparationAgricultural scienceGenetic diversity
The invention discloses a primer pair, a castanopsis hystrix SSR2 (Simple Sequence Repeat 2) marker and a preparation method and application thereof. According to the preparation method, the total DNA (Deoxyribonucleic Acid) of castanopsis hystrix is subjected to PCR (Polymerase Chain Reaction) amplification by using the specific primer pair HZ2, thereby obtaining the SSR2 marker. The method is simple and feasible and is simple and convenient in operation; shown by tests which are carried out in members of two natural castanopsis hystrix populations and among the members, the obtained SSR has relatively high polymorphism. The castanopsis hystrix SSR2 marker is a co-dominant marker; compared with other molecular markers, the SSR marker is good in repeatability and high in reliability, can be applied to the genetic diversity evaluation of castanopsis hystrix, the genetic map construction of castanopsis hystrix, the research on the spatial distribution pattern of the genetic variation of castanopsis hystrix population and the origination and evolution of castanopsis hystrix and the standardization of target genes, and can also be applied to the molecular marker assisted breeding and QTL (Quantitative Trait Locus) research of castanopsis hystrix.
Owner:GUANGXI FORESTRY RES INST
Primer pair, castanopsis hystrix SSR8 (Simple Sequence Repeat 8) marker and preparation method and application thereof
InactiveCN103740709AGood repeatabilityImprove reliabilityMicrobiological testing/measurementDNA preparationGenetic diversityCastanopsis hystrix
The invention discloses a primer pair, a castanopsis hystrix SSR8 (Simple Sequence Repeat 8) marker and a preparation method and application thereof. According to the preparation method, the total DNA (Deoxyribonucleic Acid) of castanopsis hystrix is subjected to PCR (Polymerase Chain Reaction) amplification by using the specific primer pair HZ8, thereby obtaining the SSR8 marker. The method is simple and feasible and is simple and convenient in operation; shown by tests which are carried out in members of two natural castanopsis hystrix populations and among the members, the obtained SSR has relatively high polymorphism. The castanopsis hystrix SSR8 marker is a co-dominant marker; compared with other molecular markers, the SSR marker is good in repeatability and high in reliability, can be applied to the genetic diversity evaluation of castanopsis hystrix, the genetic map construction of castanopsis hystrix, the research on the spatial distribution pattern of the genetic variation of castanopsis hystrix population and the origination and evolution of castanopsis hystrix and the standardization of target genes, and can also be applied to the molecular marker assisted breeding and QTL (Quantitative Trait Locus) research of castanopsis hystrix.
Owner:GUANGXI FORESTRY RES INST
Zelkova SSR2 mark, primer pair as well as preparation methods and application thereof
InactiveCN104450692AGood repeatabilityImprove reliabilityMicrobiological testing/measurementDNA preparationCO-DOMINANTQuantitative trait locus
The invention discloses a Zelkova SSR2 marker and a primer pair as well as preparation methods and applications thereof. PCR amplification is carried out on Zelkova total DNAs of JS2 by an inventor utilizing a specific primer, so that the SSR2 marker with a simple and repeated sequence is obtained. The method is simple and practicable and simple and convenient to operate, the obtained simple and repeated sequence shows higher polymorphism for detection inside or among populations of three Zelkova natural colonies. The Zelkova SSR2 marker is a co-dominant marker; compared with other molecular markers, the SSR marker is good in repeatability and high in reliability, can be applied to Zelkova genetic diversity evaluation, Zelkova genetic map construction, Zelkova population heritable variation spatial distribution pattern and Zelkova origin and evolution research as well as target gene marking, and can also be applied to Zelkova molecular marker assistant breeding and QTL (quantitative Trait Locus) research.
Owner:CENTRAL SOUTH UNIVERSITY OF FORESTRY AND TECHNOLOGY
A method for detecting mixed varieties of redecured tobacco
InactiveCN105256065BAvoid blind selectionImprove screening efficiencyMicrobiological testing/measurementSpectral bandsComparison standard
Owner:SOUTHWEST FORESTRY UNIVERSITY
Simple repetition sequence primer for moerella iridescens
InactiveCN101429558AStrip clearGood repeatabilityMicrobiological testing/measurementDNA/RNA fragmentationAgricultural scienceRainbow
The invention relates to a simple repetitive sequence primer for Rainbow Moerella, which is characterized in that the simple repetitive sequence primer for the Rainbow Moerella is a primer group which is formed by 10 Rainbow Moerella primers. The screened amplified products of the primer are clear and stable, have good polymorphism, can be applied in idioplasm evaluation and idioplasm identification of the Rainbow Moerella, and diversity evaluation of colonies and remains of the Rainbow Moerella, and have the characteristics of quickness, economy, accuracy, simple and convenient operation and so on.
Owner:HUAIHAI INST OF TECH +1
Primer pair, castanopsis hystrix SSR6 (Simple Sequence Repeat 6) marker and preparation method and application thereof
InactiveCN103740710AGood repeatabilityImprove reliabilityMicrobiological testing/measurementDNA preparationGenetic diversityCastanopsis hystrix
The invention discloses a primer pair, a castanopsis hystrix SSR6 (Simple Sequence Repeat 6) marker and a preparation method and application thereof. According to the preparation method, the total DNA (Deoxyribonucleic Acid) of castanopsis hystrix is subjected to PCR (Polymerase Chain Reaction) amplification by using the specific primer pair HZ6, thereby obtaining the SSR6 marker. The method is simple and feasible and is simple and convenient in operation; shown by tests which are carried out in members of two natural castanopsis hystrix populations and among the members, the obtained SSR has relatively high polymorphism. The castanopsis hystrix SSR6 marker is a co-dominant marker; compared with other molecular markers, the SSR marker is good in repeatability and high in reliability, can be applied to the genetic diversity evaluation of castanopsis hystrix, the genetic map construction of castanopsis hystrix, the research on the spatial distribution pattern of the genetic variation of castanopsis hystrix population and the origination and evolution of castanopsis hystrix and the standardization of target genes, and can also be applied to the molecular marker assisted breeding and QTL (Quantitative Trait Locus) research of castanopsis hystrix.
Owner:GUANGXI FORESTRY RES INST
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