3 results about "Simple Repetitive Sequences" patented technology

The application discloses a kind of molecular markers of wheat stripe rust SNP homozygous site based on whole genome sequence and application thereof.The application is analyzed to 28 strains of global wheat stripe rust genome sequence, compared with reference genome, and more than a million SNP sites are mined out.Sequence depth screening, linkage disequilibrium analysis and heterozygosity detection, identify 1076 SNP homozygous sites.Further based on the frequency of secondary allele, screening of deletion rate and adjacent simple repeat sequence, finally determine 37 core SNP markers.Through primer design to these SNP sites, and add fluorescent linker, develop KASP-SNP molecular marker, which can be used for accurate genotyping identification of wheat stripe rust population.The molecular marker developed based on global wheat stripe rust whole genome is suitable for wheat stripe rust population research in all regions of the world.Based on the development of molecular marker of homozygous site, polymorphism difference caused by heterozygous site can be effectively excluded, to ensure the accuracy of detection site.The set of KASP-SNP molecular marker has high polymorphism, good repeatability and high detection efficiency, and can be widely applied to genetic research of wheat stripe rust population.

The present application relates to a host cell which improves plasmid stability, and the use thereof. The present application specifically relates to an Escherichia coli host cell capable of improving the stability of a nucleic acid molecule containing a hairpin structure sequence such as ITR or a simple sequence repeat such as a poly(A) sequence, wherein the sbcC and sbcD genes of the host cell are knocked out, and the gyrA gene expresses a GyrA variant or the gyrB gene expresses a GyrB variant.

The invention discloses a molecular marker of a wheat stripe rust SNP homozygous site based on a whole genome sequence and application of the molecular marker. According to the invention, genomic sequences of 28 wheat stripe rust strains in the world are analyzed and compared with a reference genome, so that more than millions of SNP loci are mined. And through sequencing depth screening, linkage imbalance analysis and heterozygosity detection, 1076 SNP homozygous sites are identified. Furthermore, 37 core SNP markers are finally determined on the basis of secondary allele frequency, deletion rate and screening of adjacent simple repetitive sequences. The KASP-SNP molecular marker is developed by carrying out primer design on the SNP sites and adding a fluorescent linker, and can be used for carrying out accurate genotyping identification on a wheat stripe rust colony. The molecular marker developed on the basis of a global wheat stripe rust whole genome is suitable for wheat stripe rust population research in all regions in the world. On the basis of molecular marker development of a homozygous site, polymorphism difference caused by a heterozygous site can be effectively eliminated, and the accuracy of the detection site is ensured. The KASP-SNP molecular marker has high polymorphism, good repeatability and high detection efficiency, and can be widely applied to genetic research of wheat stripe rust colony.