36 results about "Simple Repetitive Sequences" patented technology

The invention discloses a method for determining quantitative trait loci (QTL) for soybean phytophthora root rot tolerance and the application of the loci, comprising the following steps: carrying out analysis (genotype data analysis) upon the DNA of the descendant of the disease tolerant variety (female parent) and the infected strain (male parent) by utilizing random primer (RAPD), repeat sequence primer (SSR) and sequence characteristic application range primer (SCAR); conducting inoculation identification upon the descendant with home and aboard mixed strain and having statistics upon the disease loss rate (phenotype data analysis); calculating the existent threshold of quantitative trait loci (QTL) for soybean phytophthora root rot tolerance with regression algorithm and detecting whether there are quantitative trait loci (QTL) for soybean phytophthora root rot tolerance with WinQTLCarter2.0; and finally judging whether the genealogies or derived varieties have the quantitative trait loci (QTL) by detecting the DNA of parent and genealogies or derived varieties thereof. The invention can detect whether the genealogies or derived varieties have disease tolerance so as to speed up the seed selection for varieties (strains) of soybean phytophthora root rot tolerance.

The invention provides a molecular identification method for cymbidium varieties, which is a simple sequence repeat genome analysis-based molecular identification method for the cymbidium varieties and analyzes the genetic relation of the cymbidium varieties by optimizing a technical system of cymbidium simple sequence repeat (SSR) analysis. By directly detecting the DNA sequence difference of the variety with genetic stability and information diversity, the method can definitely distinguish and judge different varieties of cymbidium, and realize accurate and quick identification of the variety so as to provide a reliable technique for variety identification, seed and seedling market management, variety protection and elite seed breeding and the like. By optimizing the SSR technical system of cymbidium genome DNA, the molecular identification of the cymbidium varieties has high speed, short time and multiple polymorphisms, the problems of reality inspection and the like of the cymbidium seedlings can be solved before trading, and the loss of a producer or a buyer is avoided. The method can distinguish and identify all cymbidium varieties by using one and more effective primers, and realize DNA fingerprint identification of the cymbidium varieties.

The invention relates to a method for detecting redried tobacco varietal complexity. According to the technical scheme, the method comprises the following steps of 1, extraction of redried tobacoo DNA; 2, synthesis of primers; 3, a simple repetition inter-sequence polymorphism-polymerization enzyme-linked reaction, wherein the ISSR primer 826 or the ISSR primer 856 is subjected to an ISSR-PCR in redried tobacco to be detected; 4, comparison of a standard diagram, wherein strip comparison is performed on a standard fingerprint chromatogram and an imaging picture obtained after electrophoresis of the redried tobacco ISSR-PCR to be detected is performed, if strips can be matched, the refried tobacco is in one corresponding variety, and if the strips cannot be matched, the refried tobacco is in the mixed variety, and detection is completed. The method has the advantages that the method is provided for authentication and detection of redried tobacco, the primers obtained through screening of the experiment can be directly referred to in the redried tobacco experiment process and subjected to an ISSR-PCR directly at the corresponding annealing temperature, and then the fingerprint spectrum in the corresponding variety is obtained to be used as a follow-up comparison standard spectral band.

The invention discloses a primer pair, a castanopsis hystrix SSR7 (Simple Sequence Repeat 7) marker and a preparation method and application thereof. According to the preparation method, the total DNA (Deoxyribonucleic Acid) of castanopsis hystrix is subjected to PCR (Polymerase Chain Reaction) amplification by using the specific primer pair HZ7, thereby obtaining the SSR7 marker. The method is simple and feasible and is simple and convenient in operation; shown by tests which are carried out in members of two natural castanopsis hystrix populations and among the members, the obtained SSR has relatively high polymorphism. The castanopsis hystrix SSR7 marker is a co-dominant marker; compared with other molecular markers, the SSR marker is good in repeatability and high in reliability, can be applied to the genetic diversity evaluation of castanopsis hystrix, the genetic map construction of castanopsis hystrix, the research on the spatial distribution pattern of the genetic variation of castanopsis hystrix population and the origination and evolution of castanopsis hystrix and the standardization of target genes, and can also be applied to the molecular marker assisted breeding and QTL (Quantitative Trait Locus) research of castanopsis hystrix.

The invention discloses a Zelkova SSR1 marker and a primer pair and preparation methods and applications thereof. PCR amplification is carried out on Zelkova total DNAs of JS1 by an inventor utilizing a specific primer, so that the SSR1 marker with a simple and repeated sequence is obtained. The method is simple and practicable and simple and convenient to operate, the obtained simple and repeated sequence shows higher polymorphism for detection inside or among populations of three Zelkova natural colonies. The Zelkova SSR1 marker is a co-dominant marker; compared with other molecular markers, the SSR marker is good in repeatability and high in reliability, can be applied to Zelkova genetic diversity evaluation, Zelkova genetic map construction, Zelkova population heritable variation spatial distribution pattern and Zelkova origin and evolution research as well as target gene marking, and can also be applied to Zelkova molecular marker assistant breeding and QTL (quantitative Trait Locus) research.

The invention discloses a primer pair, a castanopsis hystrix SSR5 (Simple Sequence Repeat 5) marker and a preparation method and application thereof. According to the preparation method, the total DNA (Deoxyribonucleic Acid) of castanopsis hystrix is subjected to PCR (Polymerase Chain Reaction) amplification by using the specific primer pair HZ5, thereby obtaining the SSR5 marker. The method is simple and feasible and is simple and convenient in operation; shown by tests which are carried out in members of two natural castanopsis hystrix populations and among the members, the obtained SSR has relatively high polymorphism. The castanopsis hystrix SSR5 marker is a co-dominant marker; compared with other molecular markers, the SSR marker is good in repeatability and high in reliability, can be applied to the genetic diversity evaluation of castanopsis hystrix, the genetic map construction of castanopsis hystrix, the research on the spatial distribution pattern of the genetic variation of castanopsis hystrix population and the origination and evolution of castanopsis hystrix and the standardization of target genes, and can also be applied to the molecular marker assisted breeding and QTL (Quantitative Trait Locus) research of castanopsis hystrix.

The invention discloses a primer pair, a castanopsis hystrix SSR3 (Simple Sequence Repeat 3) marker and a preparation method and application thereof. According to the preparation method, the total DNA (Deoxyribonucleic Acid) of castanopsis hystrix is subjected to PCR (Polymerase Chain Reaction) amplification by using the specific primer pair HZ3, thereby obtaining the SSR3 marker. The method is simple and feasible and is simple and convenient in operation; shown by tests which are carried out in members of two natural castanopsis hystrix populations and among the members, the obtained SSR has relatively high polymorphism. The castanopsis hystrix SSR3 marker is a co-dominant marker; compared with other molecular markers, the SSR marker is good in repeatability and high in reliability, can be applied to the genetic diversity evaluation of castanopsis hystrix, the genetic map construction of castanopsis hystrix, the research on the spatial distribution pattern of the genetic variation of castanopsis hystrix population and the origination and evolution of castanopsis hystrix and the standardization of target genes, and can also be applied to the molecular marker assisted breeding and QTL (Quantitative Trait Locus) research of castanopsis hystrix.

The invention discloses a nucleotide sequence, a nucleic acid molecular probe and a method for verifying a variant Fritillaria thunbergii.Miq.var. chekiangensis Hsiao et K.C.Hsia, and pertains to the technical field for verifying traditional Chinese medicine. The invention provides a special sequence for expanded ISSR between simple repeated sequences of Fritillaria thunbergii.Miq.var. chekiangensis Hsiao et K.C.Hsia and the verifying probe and method based on the sequence for the Fritillaria thunbergii.Miq.var. chekiangensis Hsiao et K.C.Hsia. The benefits with the invention are: 1) less sample use. The whole operation can be completed with few sample; 2) high accuracy and sensibility. The friD1/friD2 is a special molecular probe for Fritillaria thunbergii.Miq.var. chekiangensis Hsiao et K.C.Hsia, and the probe will have negative reaction for other species.

the invention discloses simple repeating sequence primer of three-wart swimming crab, which comprises the following parts: CAC ACA CAC ACA CAC AG,AGA GAG AGA GAG AGA GYT,AGA GAG AGA GAG AGA GYC,AGA GAG AGA GAG AGA GYA,GAG AGA GAG AGA GAG AYT,CAC ACA CAC ACA CAC ARC,CAC ACA CAC ACA CAC ARG, GTG TGT GTG TGT GTG TYA,GTG TGT GTG TGT GTG TYC,GTG TGT GTG TGT GTG TYG.

The invention discloses a primer pair, a castanopsis hystrix SSR4 (Simple Sequence Repeat 4) marker and a preparation method and application thereof. According to the preparation method, the total DNA (Deoxyribonucleic Acid) of castanopsis hystrix is subjected to PCR (Polymerase Chain Reaction) amplification by using the specific primer pair HZ4, thereby obtaining the SSR4 marker. The method is simple and feasible and is simple and convenient in operation; shown by tests which are carried out in members of two natural castanopsis hystrix populations and among the members, the obtained SSR has relatively high polymorphism. The castanopsis hystrix SSR4 marker is a co-dominant marker; compared with other molecular markers, the SSR marker is good in repeatability and high in reliability, can be applied to the genetic diversity evaluation of castanopsis hystrix, the genetic map construction of castanopsis hystrix, the research on the spatial distribution pattern of the genetic variation of castanopsis hystrix population and the origination and evolution of castanopsis hystrix and the standardization of target genes, and can also be applied to the molecular marker assisted breeding and QTL (Quantitative Trait Locus) research of castanopsis hystrix.

The invention discloses a primer pair, a castanopsis hystrix SSR2 (Simple Sequence Repeat 2) marker and a preparation method and application thereof. According to the preparation method, the total DNA (Deoxyribonucleic Acid) of castanopsis hystrix is subjected to PCR (Polymerase Chain Reaction) amplification by using the specific primer pair HZ2, thereby obtaining the SSR2 marker. The method is simple and feasible and is simple and convenient in operation; shown by tests which are carried out in members of two natural castanopsis hystrix populations and among the members, the obtained SSR has relatively high polymorphism. The castanopsis hystrix SSR2 marker is a co-dominant marker; compared with other molecular markers, the SSR marker is good in repeatability and high in reliability, can be applied to the genetic diversity evaluation of castanopsis hystrix, the genetic map construction of castanopsis hystrix, the research on the spatial distribution pattern of the genetic variation of castanopsis hystrix population and the origination and evolution of castanopsis hystrix and the standardization of target genes, and can also be applied to the molecular marker assisted breeding and QTL (Quantitative Trait Locus) research of castanopsis hystrix.

The invention discloses a capture probe set for detecting food allergens as well as a preparation method and application of the capture probe set. The preparation method comprises the following steps of comparing gene segments of at least two allergens in pairs according to sequence similarity; classifying genes with the lengths of more than 120bp and the similarity of not less than 90% among allergen genes into one type; carrying out classification treatment to finally obtain at least one type of target genes, and carrying out multi-sequence comparison on the target genes by utilizing clustalw2 software; obtaining a consensus sequence of each type of target genes as a candidate probe by using a BioPerl module of a Perl language; and for each candidate probe, sequentially intercepting a sequence with the length of 100-130bp, and taking the probe which has the CG content of 30-70% and does not contain simple repetitive sequences as a single-stranded probe. The invention has the beneficial effects that high-throughput detection of sensitization components and sensitization pollutants in a complex food system can be realized, and the application value is very high.

The invention discloses an SSR (Simple Sequence Repeat) marker for simultaneously identifying sterile cytoplasm of gossypium harknessii and gossypium hirsutum. Primers of the SSR marker are shown as SEQ ID NO.1-2, and the SSR marker is not only linked with a gossypium harknessii cytoplasmic male sterility gene, but also linked with a gossypium hirsutum cytoplasmic male sterility gene. The simple repetitive sequence polymorphism of the nucleotide is presented on a polyacrylamide electrophoretogram, whether a single cotton plant contains a sterile gene or not can be identified according to an amplification band, and the type (CMS-D2/CMS-D8) containing the sterile gene can be judged. By utilizing the same SSR marker, not only can the sterile line and the maintainer line of a cotton three-line material be identified, but also two sets of cytoplasmic male sterility genes of the cotton can be distinguished at the same time, and hybrid seeds matched with different three lines can be identified. The SSR marker plays an important role in breeding new cotton materials with different sterile genes and ensuring the purity of three-line hybrid seeds.

The invention discloses hibiscus cannabinus L. drought response gene EST-SSR primers and a kit. 11 pairs of primers are provided, and one pair or multiple pairs of primers are used as EST-SSR polymorphism markers for identifying the genetic relationship of hibiscus cannabinus L., the genetic diversity or the differences of simple repeat sequence polymorphisms in specific exon regions of drought-related genes. The hibiscus cannabinus L. drought response gene EST-SSR primers and the kit disclosed by the invention have the benefits that a good marker reserve is provided for molecular marker-assisted breeding of the hibiscus cannabinus L., and a candidate molecular marker for detection is also provided for functional allelic variation of key genes screened in the natural variation of the hibiscus cannabinus L.

The invention discloses a primer pair, a castanopsis hystrix SSR8 (Simple Sequence Repeat 8) marker and a preparation method and application thereof. According to the preparation method, the total DNA (Deoxyribonucleic Acid) of castanopsis hystrix is subjected to PCR (Polymerase Chain Reaction) amplification by using the specific primer pair HZ8, thereby obtaining the SSR8 marker. The method is simple and feasible and is simple and convenient in operation; shown by tests which are carried out in members of two natural castanopsis hystrix populations and among the members, the obtained SSR has relatively high polymorphism. The castanopsis hystrix SSR8 marker is a co-dominant marker; compared with other molecular markers, the SSR marker is good in repeatability and high in reliability, can be applied to the genetic diversity evaluation of castanopsis hystrix, the genetic map construction of castanopsis hystrix, the research on the spatial distribution pattern of the genetic variation of castanopsis hystrix population and the origination and evolution of castanopsis hystrix and the standardization of target genes, and can also be applied to the molecular marker assisted breeding and QTL (Quantitative Trait Locus) research of castanopsis hystrix.

The invention discloses a Zelkova SSR2 marker and a primer pair as well as preparation methods and applications thereof. PCR amplification is carried out on Zelkova total DNAs of JS2 by an inventor utilizing a specific primer, so that the SSR2 marker with a simple and repeated sequence is obtained. The method is simple and practicable and simple and convenient to operate, the obtained simple and repeated sequence shows higher polymorphism for detection inside or among populations of three Zelkova natural colonies. The Zelkova SSR2 marker is a co-dominant marker; compared with other molecular markers, the SSR marker is good in repeatability and high in reliability, can be applied to Zelkova genetic diversity evaluation, Zelkova genetic map construction, Zelkova population heritable variation spatial distribution pattern and Zelkova origin and evolution research as well as target gene marking, and can also be applied to Zelkova molecular marker assistant breeding and QTL (quantitative Trait Locus) research.

The invention relates to a simple repetitive sequence primer for Rainbow Moerella, which is characterized in that the simple repetitive sequence primer for the Rainbow Moerella is a primer group which is formed by 10 Rainbow Moerella primers. The screened amplified products of the primer are clear and stable, have good polymorphism, can be applied in idioplasm evaluation and idioplasm identification of the Rainbow Moerella, and diversity evaluation of colonies and remains of the Rainbow Moerella, and have the characteristics of quickness, economy, accuracy, simple and convenient operation and so on.

The invention discloses a primer pair, a castanopsis hystrix SSR6 (Simple Sequence Repeat 6) marker and a preparation method and application thereof. According to the preparation method, the total DNA (Deoxyribonucleic Acid) of castanopsis hystrix is subjected to PCR (Polymerase Chain Reaction) amplification by using the specific primer pair HZ6, thereby obtaining the SSR6 marker. The method is simple and feasible and is simple and convenient in operation; shown by tests which are carried out in members of two natural castanopsis hystrix populations and among the members, the obtained SSR has relatively high polymorphism. The castanopsis hystrix SSR6 marker is a co-dominant marker; compared with other molecular markers, the SSR marker is good in repeatability and high in reliability, can be applied to the genetic diversity evaluation of castanopsis hystrix, the genetic map construction of castanopsis hystrix, the research on the spatial distribution pattern of the genetic variation of castanopsis hystrix population and the origination and evolution of castanopsis hystrix and the standardization of target genes, and can also be applied to the molecular marker assisted breeding and QTL (Quantitative Trait Locus) research of castanopsis hystrix.