The invention relates to a method for detecting redried tobacco varietal complexity. According to the technical scheme, the method comprises the following steps of 1, extraction of redried tobacoo DNA; 2, synthesis of primers; 3, a simple repetition inter-sequence polymorphism-polymerization enzyme-linked reaction, wherein the ISSR primer 826 or the ISSR primer 856 is subjected to an ISSR-PCR in redried tobacco to be detected; 4, comparison of a standard diagram, wherein strip comparison is performed on a standard fingerprint chromatogram and an imaging picture obtained after electrophoresis of the redried tobacco ISSR-PCR to be detected is performed, if strips can be matched, the refried tobacco is in one corresponding variety, and if the strips cannot be matched, the refried tobacco is in the mixed variety, and detection is completed. The method has the advantages that the method is provided for authentication and detection of redried tobacco, the primers obtained through screening of the experiment can be directly referred to in the redried tobacco experiment process and subjected to an ISSR-PCR directly at the corresponding annealing temperature, and then the fingerprint spectrum in the corresponding variety is obtained to be used as a follow-up comparison standard spectral band.