Kenaf drought response gene est-ssr primer and kit
A kit and gene technology, applied in biochemical equipment and methods, microbiological determination/inspection, DNA/RNA fragments, etc., can solve problems affecting the accuracy of genetic diversity analysis of germplasm sources and achieve good marker reserves Effect
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[0024] The EST-SSR markers of key genes in the metabolic pathways of kenaf in response to drought stress are used for the sequencing of kenaf drought stress transcriptomes, the enrichment analysis of metabolic pathways in response to drought stress, the development of EST-SSR markers, DNA extraction, SSR primer screening and variety identification, etc. obtained by the process ( figure 1 ).
[0025] In the present invention, using the kenaf transcriptome sequencing data to obtain the drought stress key gene development kenaf EST-SSR primer specific method is:
[0026] 1. Drought stress transcriptome sequencing of kenaf
[0027] Using RNAprep Pure Plant Kit (Tiangen Biotechnology, China) from the control group (normal watering for 6 days), drought treatment group (suppressed watering for 6 days), rewatering treatment group (suppressed watering for 5 days and rewatering for 1 day) Total RNA was extracted from kenaf leaves and repeated 3 times. RNA quality, concentration and ...
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