A method for detecting mixed varieties of redecured tobacco
A technology for red-cured tobacco and varieties, applied in the directions of biochemical equipment and methods, microbial determination/inspection, etc., can solve the problems of mixed varieties, mislabeled, affecting the quality and style stability of cloud-produced cigarettes, and achieves the goal of improving screening efficiency. Effect
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[0044] A kind of method that the present invention mentions to detect the mixed variety of redecured tobacco, comprises the following steps:
[0045] (1) Extraction of DNA from re-cured tobacco: DNeasy kit was used to extract DNA, and then the integrity of DNA was detected by electrophoresis on 1.0% agarose gel, and the purity and concentration of DNA extraction were detected by spectrophotometer; among them, DNeasy kit was produced by The commercially available product of QIAGEN company of Germany;
[0046] (2) Primer synthesis: Synthesize ISSR primer 826 and ISSR primer 856, wherein, the primer sequence of ISSR primer 826: ACA CAC ACA CAC ACA CC; the primer sequence of ISSR primer 856: ACA CAC ACA CAC ACA CYA, after synthesis, use ddH2O dissolve the primers;
[0047] (3) Inter-simple repeat polymorphism-polymerase chain reaction, abbreviated as ISSR-PCR:
[0048] Using ISSR primer 826 or ISSR primer 856 to perform ISSR-PCR in the re-cured tobacco to be detected;
[0049] ...
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