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A method for detecting mixed varieties of redecured tobacco

A technology for red-cured tobacco and varieties, applied in the directions of biochemical equipment and methods, microbial determination/inspection, etc., can solve the problems of mixed varieties, mislabeled, affecting the quality and style stability of cloud-produced cigarettes, and achieves the goal of improving screening efficiency. Effect

Inactive Publication Date: 2019-01-29
SOUTHWEST FORESTRY UNIVERSITY
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

So far, the identification of differences among tobacco leaf varieties has only been evaluated based on the appearance quality of tobacco leaves. In the process of tobacco leaf production, purchase, rebaking, and storage, a scientific tracking and monitoring system has not been formed, resulting in mixed varieties, wrong The phenomenon of standardization has seriously affected the stability of the quality and style of cigarettes produced in Yunzhong, such as: Red-flowered Jinyuan, Yun87, NC297, NC102, K326, etc.

Method used

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  • A method for detecting mixed varieties of redecured tobacco
  • A method for detecting mixed varieties of redecured tobacco
  • A method for detecting mixed varieties of redecured tobacco

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Embodiment Construction

[0044] A kind of method that the present invention mentions to detect the mixed variety of redecured tobacco, comprises the following steps:

[0045] (1) Extraction of DNA from re-cured tobacco: DNeasy kit was used to extract DNA, and then the integrity of DNA was detected by electrophoresis on 1.0% agarose gel, and the purity and concentration of DNA extraction were detected by spectrophotometer; among them, DNeasy kit was produced by The commercially available product of QIAGEN company of Germany;

[0046] (2) Primer synthesis: Synthesize ISSR primer 826 and ISSR primer 856, wherein, the primer sequence of ISSR primer 826: ACA CAC ACA CAC ACA CC; the primer sequence of ISSR primer 856: ACA CAC ACA CAC ACA CYA, after synthesis, use ddH2O dissolve the primers;

[0047] (3) Inter-simple repeat polymorphism-polymerase chain reaction, abbreviated as ISSR-PCR:

[0048] Using ISSR primer 826 or ISSR primer 856 to perform ISSR-PCR in the re-cured tobacco to be detected;

[0049] ...

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Abstract

The invention relates to a method for detecting redried tobacco varietal complexity. According to the technical scheme, the method comprises the following steps of 1, extraction of redried tobacoo DNA; 2, synthesis of primers; 3, a simple repetition inter-sequence polymorphism-polymerization enzyme-linked reaction, wherein the ISSR primer 826 or the ISSR primer 856 is subjected to an ISSR-PCR in redried tobacco to be detected; 4, comparison of a standard diagram, wherein strip comparison is performed on a standard fingerprint chromatogram and an imaging picture obtained after electrophoresis of the redried tobacco ISSR-PCR to be detected is performed, if strips can be matched, the refried tobacco is in one corresponding variety, and if the strips cannot be matched, the refried tobacco is in the mixed variety, and detection is completed. The method has the advantages that the method is provided for authentication and detection of redried tobacco, the primers obtained through screening of the experiment can be directly referred to in the redried tobacco experiment process and subjected to an ISSR-PCR directly at the corresponding annealing temperature, and then the fingerprint spectrum in the corresponding variety is obtained to be used as a follow-up comparison standard spectral band.

Description

technical field [0001] The invention relates to a detection method of recured tobacco, in particular to a method for detecting mixed varieties of recured tobacco. Background technique [0002] Flue-cured tobacco is the raw material after the first processing of plants, and re-cured tobacco is the preparatory process of sorting and processing tobacco leaves from agricultural products into industrial raw materials. It is another drying after tobacco leaves are prepared, so it is called re-cured. During the re-curing process of tobacco leaves, moldy and unqualified tobacco leaves should be removed to ensure the quality of re-cured tobacco. The pests of the tobacco leaves can be killed while heating and drying, and the moisture content of the rebaked tobacco leaves is very low, which is good for long-term storage, alcoholization or artificial fermentation. Leaf threshing and redrying is a key link in providing high-quality raw materials for cigarette companies. At present, tob...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6858
CPCC12Q1/6895C12Q2600/156
Inventor 吴田张奇
Owner SOUTHWEST FORESTRY UNIVERSITY
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