Zelkova SSR1 marker and primer pair and preparation methods and application thereof

A primer pair, zelkova technology, applied in biochemical equipment and methods, microbial determination/inspection, DNA/RNA fragments, etc., can solve problems such as poor stability
CN104450693AInactive Publication Date: 2015-03-25CENTRAL SOUTH UNIVERSITY OF FORESTRY AND TECHNOLOGY
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
CENTRAL SOUTH UNIVERSITY OF FORESTRY AND TECHNOLOGY
Publication Date
2015-03-25
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention discloses a Zelkova SSR1 marker and a primer pair and preparation methods and applications thereof. PCR amplification is carried out on Zelkova total DNAs of JS1 by an inventor utilizing a specific primer, so that the SSR1 marker with a simple and repeated sequence is obtained. The method is simple and practicable and simple and convenient to operate, the obtained simple and repeated sequence shows higher polymorphism for detection inside or among populations of three Zelkova natural colonies. The Zelkova SSR1 marker is a co-dominant marker; compared with other molecular markers, the SSR marker is good in repeatability and high in reliability, can be applied to Zelkova genetic diversity evaluation, Zelkova genetic map construction, Zelkova population heritable variation spatial distribution pattern and Zelkova origin and evolution research as well as target gene marking, and can also be applied to Zelkova molecular marker assistant breeding and QTL (quantitative Trait Locus) research.
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Description

technical field

[0001] The invention belongs to the technical field of molecular markers in biological sciences, in particular to a beech SSR1 marker, a pair of primers and a preparation method and application thereof. Background technique

[0002] Zelkova schneideriana Hand.-Mazz. is a large deciduous tree belonging to the genus Zelkova in the family Ulmaceae, endemic to my country. Beech trees are distributed in the Huaihe River Basin in my country and the middle and lower reaches of the Yangtze River south of the Qinling Mountains. Beech wood is dense and hard, with bright color, beautiful texture and strong corrosion resistance. It is the first-class wood for the production of various high-end furniture and handicrafts. At the same time, because of its beautiful tree shape, large canopy, and rich seasonal changes in leaf color, it is also a popular traditional color-leaf garden landscape tree species. Due to the low germination rate of beech seeds, the difficulty of na...

Claims

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