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Hibiscus cannabinus L. drought response gene EST-SSR primers and kit

A kenaf and gene technology, applied in the field of EST-SSR primers and kits for kenaf drought-responsive genes, and EST-SSR markers, can solve the problems affecting the accuracy of genetic diversity analysis of germplasm sources, and achieve a good marker reserve. Effect

Active Publication Date: 2018-05-18
ZHEJIANG XIAOSHAN COTTON & FLAX RES INST
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Problems solved by technology

However, the quantity and quality of molecular markers affect the accuracy of genetic diversity analysis of germplasm sources, and the existing kenaf SSR molecular markers are far from meeting the needs

Method used

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  • Hibiscus cannabinus L. drought response gene EST-SSR primers and kit
  • Hibiscus cannabinus L. drought response gene EST-SSR primers and kit
  • Hibiscus cannabinus L. drought response gene EST-SSR primers and kit

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Embodiment Construction

[0024] The EST-SSR markers of key genes in the metabolic pathways of kenaf in response to drought stress are used for the sequencing of kenaf drought stress transcriptomes, the enrichment analysis of metabolic pathways in response to drought stress, the development of EST-SSR markers, DNA extraction, SSR primer screening and variety identification, etc. obtained by the process ( figure 1 ).

[0025] In the present invention, using the kenaf transcriptome sequencing data to obtain the drought stress key gene development kenaf EST-SSR primer specific method is:

[0026] 1. Drought stress transcriptome sequencing of kenaf

[0027] Using RNAprep Pure Plant Kit (Tiangen Biotechnology, China) from the control group (normal watering for 6 days), drought treatment group (suppressed watering for 6 days), rewatering treatment group (suppressed watering for 5 days and rewatering for 1 day) Total RNA was extracted from kenaf leaves and repeated 3 times. RNA quality, concentration and ...

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Abstract

The invention discloses hibiscus cannabinus L. drought response gene EST-SSR primers and a kit. 11 pairs of primers are provided, and one pair or multiple pairs of primers are used as EST-SSR polymorphism markers for identifying the genetic relationship of hibiscus cannabinus L., the genetic diversity or the differences of simple repeat sequence polymorphisms in specific exon regions of drought-related genes. The hibiscus cannabinus L. drought response gene EST-SSR primers and the kit disclosed by the invention have the benefits that a good marker reserve is provided for molecular marker-assisted breeding of the hibiscus cannabinus L., and a candidate molecular marker for detection is also provided for functional allelic variation of key genes screened in the natural variation of the hibiscus cannabinus L.

Description

technical field [0001] The invention relates to EST-SSR markers, in particular to kenaf drought response gene EST-SSR primers and a kit, belonging to the field of biotechnology. Background technique [0002] Kenaf ( Hibiscus cannabinus L . ) is an important annual bast fiber crop of the Malvaceae Hibiscus genus, which is mainly used for textiles, but also for making pulp, biomass energy, building materials, etc. Therefore, vigorously developing hemp crops, especially kenaf, is one of the important ways to meet people's ever-increasing demand for natural fibers. Kenaf has strong growth adaptability, especially drought resistance, and can be planted in hilly areas without competing for land with grain. With the vicious changes in the global climate and the increasing shortage of water resources, it is becoming more and more important to breed kenaf varieties with strong drought resistance. [0003] EST-SSR marker is a new type of molecular marker developed for simple seque...

Claims

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Application Information

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IPC IPC(8): C12Q1/6895C12N15/11
CPCC12Q1/6895C12Q2600/13C12Q2600/156
Inventor 安霞金关荣王斌吴文嫱骆霞虹陈常理李文略李苹芳朱关林
Owner ZHEJIANG XIAOSHAN COTTON & FLAX RES INST
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