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Food-tolerance-specific IgG detection kit based on multiple microarrays

A detection kit and the technology of the kit, which are applied in the field of immune detection, can solve the problems of not being able to meet the requirements of rapid detection and popularization, and it is difficult to reduce the time and manufacturing cost of food intolerance screening, so as to achieve good clinical application value and solve problems. The detection is laborious and the effect of improving the detection efficiency

Pending Publication Date: 2020-01-10
GUANGZHOU BIOHOP TECH INC
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Traditional ELISA is difficult to reduce the time and manufacturing cost of food intolerance screening, and cannot meet the requirements of on-site rapid detection and popularization

Method used

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  • Food-tolerance-specific IgG detection kit based on multiple microarrays
  • Food-tolerance-specific IgG detection kit based on multiple microarrays
  • Food-tolerance-specific IgG detection kit based on multiple microarrays

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0043] Example 1 Multiplex Microarray Food Intolerance Specific IgG Detection Kit

[0044] 1. Multiple Microarray Food Intolerance Specific IgG Detection Kit

[0045] Food intolerance-specific IgG detection kit for multiple microarrays, including: microwell plate coated with food antigen protein, washing buffer, blocking solution, enzyme-labeled antibody, human IgG standard, and chromogenic solution.

[0046]The microwell plate coated with food antigen protein is made of polystyrene and contains 96 detachable microwells. Each microwell contains a 10×10 matrix microarray. The microarray contains 90 food antigens, 6 calibration points, 2 positive controls, 2 negative controls, such as figure 1 .

[0047] The 90 food antigens involve meat and poultry, seafood and aquatic products, nuts, eggs and milk, fruits, vegetables, beans, grains, condiments and beverages.

[0048] Meat and poultry: lamb, beef, pork, chicken, duck;

[0049] Seafood and aquatic products: cod, cuttlefish,...

Embodiment 2

[0066] Example 2 Detection steps of multiple microarray food intolerance specific IgG detection kit

[0067] Analytical Reagents and Instruments:

[0068] Washing buffer: TBST solution (1×) containing 0.1% Tween 20, adjust the pH to 7.4;

[0069] Blocking solution: 1% bovine serum albumin (V)-PBS, pH 7.4;

[0070] Enzyme-labeled antibody: HRP anti-human IgG (Fc-specific) was purchased from Southern Biotech, Cat. No. 6140-05; diluted with pH 7.40, 1M PBS containing 1% calf serum, the dilution ratio was 1:1000; the concentration was 0.5 mg / L;

[0071] Human IgG standard substance: IgG (Abcam, ab97225) was formulated with pH 7.4 and 1M PBS containing 1% calf serum to prepare five different concentrations as the standard substance. The standard concentration was 0U / mL, 50U / mL, 100U / mL mL, 200U / mL, 400U / mL, the standard is calibrated by international quality control serum;

[0072] Chromogenic solution: citrate buffer containing 0.48g / L TMB (Thermo Scientific, catalog number is...

Embodiment 3

[0087] Example 3 Performance Detection of Multiplex Microarray Food Intolerance Specific IgG Detection Kit

[0088] 1. Precision measurement

[0089] Taking wheat and milk as examples, negative sera, weakly positive sera and moderately positive sera were selected from clinical samples, and the intra-assay and inter-assay tests were performed 10 times respectively. The results are shown in Table 2 (wheat) and Table 3 (milk). .

[0090] Table 2 Wheat-specific IgG precision determination

[0091]

[0092] Table 3 Milk-specific IgG precision determination

[0093]

[0094] From the results in Table 2 and Table 3, it can be seen that the kit of the present invention has good precision, meets the basic requirements of immunodiagnostic reagents, and can meet the technical needs of clinical testing in China.

[0095] 2. Compare the results with the reference reagent

[0096] The food-specific IgG antibody detection kit (14 items) of Jiangsu Hao Oubo Biomedical Co., Ltd. of C...

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Abstract

The invention discloses a food-tolerance-specific IgG detection kit based on multiple microarrays, and belongs to the field of immune detection. The food-tolerance-specific IgG detection kit based onmultiple microarrays provided by the invention comprises a microwell plate coated with a food antigen protein, a washing buffer, a blocking solution, an enzyme-labeled antibody, a human IgG standard and a coloring solution. The detection kit of the invention can detect dozens of food allergens at the same time, has the advantages of high accuracy and high sensitivity, also solves the problem of laborious and high cost of detection of a single indicator at the same time, greatly improves the detection efficiency, and has a very good clinical application value.

Description

technical field [0001] The invention belongs to the field of immune detection, in particular to a food intolerance specific IgG detection kit based on multiple microarrays. Background technique [0002] Allergic disease is a common disease, which is caused by widely existing allergens, and about 5-10% of people are infested by allergens. The substance that triggers an allergic reaction is called an allergen, and food is one of the important allergens. Food allergies can be caused by human body sensitivity to food. At present, there are two main types: the immediate allergic reaction mediated by IgE, with rapid onset and obvious symptoms; the delayed allergic reaction mediated by IgG, usually after contact with food. Reaction symptoms will appear after a few hours or a few days, such as digestive tract symptoms (abdominal pain, diarrhea, bad breath, oral ulcers, flatulence and other gastrointestinal dysfunction), skin symptoms (eczema, urticaria), respiratory symptoms (asthm...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/68G01N33/564
CPCG01N33/6854G01N33/564G01N33/6893G01N2800/24
Inventor 张凯东刘旭昭卢章准何翠红李文哲任立芬熊晓明
Owner GUANGZHOU BIOHOP TECH INC
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