The application discloses a luminescent
cell for rapidly detecting
drug allergy and / or
allergic reaction, and a construction method and application thereof, and relates to the technical field of
biotechnology.The construction method comprises the following steps: transforming a myeloid-related
cell with a recombinant vector containing a
marker gene and an H_
HEXA gene promoter to obtain a recombinant
cell; and the recombinant cell is the luminescent cell.The application is characterized in that the H_
HEXA gene promoter is recombined with the
marker gene, and then is transformed into the myeloid-related cell, and the specific activation characteristics of the H_
HEXA gene in the
allergic reaction /
allergic reaction are utilized, so that the occurrence of the allergic reaction or the allergic reaction can be directly reflected through a marker
signal.The luminescent cell has good stability, is suitable for
drug allergy, allergic reaction
risk assessment, environmental sample analysis and other scenes, and provides an efficient, sensitive and convenient technical tool for clinical rapid diagnosis, new
drug research and development and scientific research exploration, and can significantly reduce detection cost and operation threshold.