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Method for detecting anaphylactoid reaction caused by chlorogenic acids

A technology for allergic reactions and acids, applied in biochemical equipment and methods, microbial determination/inspection, etc., can solve the problems of cumbersome operation, short half-life, lack of a series of biological information, etc., saving manpower and material resources, saving testing time, Easy-to-use effects

Inactive Publication Date: 2015-05-20
BIOMEDICAL ANALYSIS CENT OF ACADEMY OF MILITARY MEDICAL SCI +1
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

As a main medicinal ingredient, chlorogenic acid is also a suspicious sensitizer, but it has not yet been determined whether this type of ingredient is an allergen, and its objective truth needs to be clarified urgently! At present, there is a lack of sufficiently reliable animal models for the detection of anaphylactoid reactions, and it is difficult to establish corresponding evaluation methods
The newly developed mouse auricle blue dye test and blood pressure test method have yet to be resolved in terms of species differences, reaction chain termination, reproducibility, etc. Allergic media or scanning / transmission electron microscopy detection, due to short half-life or chemical fixation , color processing, cumbersome operation, and the loss of a series of biological information due to the death of cells; only a certain phase can be observed, and there is no continuous dynamic monitoring

Method used

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  • Method for detecting anaphylactoid reaction caused by chlorogenic acids
  • Method for detecting anaphylactoid reaction caused by chlorogenic acids
  • Method for detecting anaphylactoid reaction caused by chlorogenic acids

Examples

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Embodiment 1

[0046] Embodiment 1: The method of the present invention for detecting anaphylactoid reactions caused by chlorogenic acid components. The chlorogenic acid components include chlorogenic acid, isochlorogenic acid A, isochlorogenic acid B, isochlorogenic acid C, and cryptochlorogenic acid or neochlorogenic acid, the method of the present invention can simultaneously monitor different concentrations and various chlorogenic acid monomers causing anaphylactoid reactions, and anaphylactoid reactions are chemically or pharmacologically mediated without the participation of the immune system. Yes, a clinical reaction with symptoms similar to anaphylaxis is shown at the first administration. Example 1 cites a method for detecting anaphylactoid reactions caused by chlorogenic acid solutions. The real-time cell analyzers used in this example are all DP-type real-time cell analyzers.

[0047] 1. Instruments used in the test: RTCA DP (German Roche Company); E-Plate 16 detection plate (6x6 ...

Embodiment 2

[0057] Embodiment two: what this embodiment enumerates is to monitor the growth index value of RBL-2H3 cell in the isochlorogenic acid A solution under different concentrations, the test instrument used is the same as embodiment one, isochlorogenic acid A reference substance (purity greater than 98 %), purchased from Chengdu Master Biotechnology Co., Ltd.

[0058] The steps of the method for detecting anaphylactoid reaction caused by isochlorogenic acid A solution at different concentrations include the following steps, including the following steps, (1) preparing RBL-2H3 cell suspension and isochlorogenic acid A solution of different concentrations respectively; ( 2) Use the real-time cell analyzer to monitor the cell growth index in the RBL-2H3 suspension with different cell concentrations, and determine the RBL-2H3 cell suspension used when the real-time cell analyzer detects the allergic reaction caused by the isochlorogenic acid A solution The optimal cell concentration a...

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Abstract

The invention discloses a method for detecting anaphylactoid reaction caused by chlorogenic acids. The method comprises the following steps of (1) respectively preparing RBL-2H3 cell suspension and chlorogenic acid solution which are different in concentration; (2) determining the optimal cell concentration of the RBL-2H3 cell suspension and the optimal time point of addition of the chlorogenic acid solution when a real-time cell analyzer detects the anaphylactoid reaction caused by chlorogenic acids; and (3) detecting the RBL-2H3 cell growth index values before and after addition of the chlorogenic acid solution by utilizing a real-time cell analyzer. The method has the advantages that growth, extension, morphologic change and death physiological states of the RBL-2H3 cell before and after the chlorogenic acid solution is added can be reflected in real time; and the concentration range of the solution of the chlorogenic acids causing the anaphylactoid reaction can be obtained.

Description

technical field [0001] The invention belongs to the technical field of drug detection, in particular to a method for detecting anaphylactoid reactions caused by chlorogenic acid components. Background technique [0002] Chlorogenic acid is a phenylpropanoid compound produced by plants through the shikimic acid pathway during aerobic respiration. It is an important biologically active substance with antibacterial, antiviral, exciting central nervous system and scavenging free radicals effect. Chinese medicine injections containing chlorogenic acid components, such as Shuanghuanglian injection and Qingkailing injection, account for about 22% of the number of listed Chinese medicine injections, and their clinical curative effect is definite, but adverse reactions occur from time to time (mainly allergic reactions, Among them, class anaphylaxis accounts for about 70%-80%). As a main medicinal ingredient, chlorogenic acid is also a suspicious sensitizer, but it has not yet been...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/02
Inventor 鄢丹马丽娜张乐乐韩玉梅张毅肖小河
Owner BIOMEDICAL ANALYSIS CENT OF ACADEMY OF MILITARY MEDICAL SCI
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