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Method for detecting substance inducing anaphylactoid reaction and kit thereof

An allergic reaction and kit technology, applied in the field of drug testing, can solve problems such as untrue test data, unsuitable reaction, instability, etc., and achieve the effect of simple and easy detection method, wide application value, and accurate and reliable results

Active Publication Date: 2017-02-22
JIANGSU KANION PHARMA CO LTD
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the calcium flow method is only suitable for the detection of adherent cells. If suspension or semi-suspension cells are used, the detection data will be unreal and unstable.
At the same time, calcium flux detection can only target compounds that quickly bind to receptors and exert their effects, and are not suitable for compounds that respond slowly

Method used

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  • Method for detecting substance inducing anaphylactoid reaction and kit thereof
  • Method for detecting substance inducing anaphylactoid reaction and kit thereof
  • Method for detecting substance inducing anaphylactoid reaction and kit thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0039] experimental method:

[0040] 1. Materials

[0041] Reagents: RPMI1640 medium (GIBCO); fetal bovine serum (Gibco), cAMP dynamic2 kit (cisbio), IP1Tb kit (cisbio), 384-well plate (Corning), Compound48 / 80 (Sigma, USA), β-D-glucosamine Amine (Sigma), Shenmai Injection (Hebei Shenwei Pharmaceutical Co., Ltd., batch number: 1401192), Xuesaitong for injection (Heilongjiang Zhenbaodao Pharmaceutical Co., Ltd., batch number: 14ka222), Mailuoning Injection (Jinling Pharmaceutical Co., Ltd. Nanjing Jinling Pharmaceutical Factory Co., Ltd., batch number: 20140403), Shuxuening (Heilongjiang Zhenbaodao Pharmaceutical Co., Ltd., batch number: B20140707), Tanreqing Injection (Shanghai Kaibao Pharmaceutical Co., Ltd., batch number: 1502204), Reduning injection (Jiangsu Kangyuan Pharmaceutical Co., Ltd., batch number: 20140722).

[0042] Cells: P815 mouse mastocytoma cells were purchased from the National Experimental Cell Resource Sharing Platform.

[0043] Instruments: carbon dioxi...

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Abstract

The invention relates to the technical field of drug testing, in particular to a method for detecting a substance inducing anaphylactoid reaction and a kit thereof. The method comprises the following steps: inoculating P815 cells into a culture medium; adding a to-be-detected object and a biological molecule specifically bonded with IP1 and / or cAMP to the culture medium, and detecting concentration of IP1 and / or cAMP; and according to the concentration of IP1 and / or cAMP, determining whether the to-be-detected object is the substance inducing the anaphylactoid reaction or not. The detection method provided by the invention has the advantages that the result is accurate and reliable and is basically consistent with the detection result of the traditional beta-hexosaminidase release rate experiment, but the sensitivity is higher than that of a beta-hexosaminidase release rate detection method; and the detection method provided by the invention is simple and quick, overcomes the defects of the traditional method, can be applied to early stage rapid screening and identification of the substance inducing the anaphylactoid reaction and has extensive value in use.

Description

technical field [0001] The invention relates to the technical field of drug detection, in particular to a method for detecting substances that cause anaphylactoid reactions and a kit thereof. Background technique [0002] Allergic reaction is allergic reaction, which is an abnormal immune reaction between exogenous antigenic substances and antibodies in the body, and is one of the common adverse reactions. There are many substances that can induce allergic reactions, such as macromolecular substances such as proteins, polypeptides, and polysaccharides with complete antigenicity; other compounds with smaller molecules can be used as haptens to combine with proteins in the body to form complete antigens, thereby causing allergic reactions. At present, there are many allergic substances involved in allergic reactions, among which medicines (traditional Chinese medicine and western medicine), food, pollen, mites, etc. occur more frequently. Acute allergic reactions include type...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/53
CPCG01N33/53G01N33/94
Inventor 萧伟江益平吴秀曹亮丁岗王振中
Owner JIANGSU KANION PHARMA CO LTD
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