A method and kit for detecting substances causing anaphylactoid reactions
A technology for allergic reactions and substances, applied in the direction of biological testing, material inspection products, etc., can solve problems such as unsuitable reactions, untrue and unstable test data, and achieve wide application value. The detection method is simple and easy, and the results are accurate and reliable. Effect
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[0039] experimental method:
[0040] 1. Materials
[0041] Reagents: RPMI1640 medium (GIBCO); fetal bovine serum (Gibco), cAMP dynamic2 kit (cisbio), IP1Tb kit (cisbio), 384-well plate (Corning), Compound48 / 80 (Sigma, USA), β-D-glucosamine Amine (Sigma), Shenmai Injection (Hebei Shenwei Pharmaceutical Co., Ltd., batch number: 1401192), Xuesaitong for injection (Heilongjiang Zhenbaodao Pharmaceutical Co., Ltd., batch number: 14ka222), Mailuoning Injection (Jinling Pharmaceutical Co., Ltd. Nanjing Jinling Pharmaceutical Factory Co., Ltd., batch number: 20140403), Shuxuening (Heilongjiang Zhenbaodao Pharmaceutical Co., Ltd., batch number: B20140707), Tanreqing Injection (Shanghai Kaibao Pharmaceutical Co., Ltd., batch number: 1502204), Reduning injection (Jiangsu Kangyuan Pharmaceutical Co., Ltd., batch number: 20140722).
[0042] Cells: P815 mouse mastocytoma cells were purchased from the National Experimental Cell Resource Sharing Platform.
[0043] Instruments: carbon dioxi...
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