A method and kit for detecting substances causing anaphylactoid reactions

Abstract

The invention relates to the technical field of drug testing, in particular to a method for detecting a substance inducing anaphylactoid reaction and a kit thereof. The method comprises the following steps: inoculating P815 cells into a culture medium; adding a to-be-detected object and a biological molecule specifically bonded with IP1 and / or cAMP to the culture medium, and detecting concentration of IP1 and / or cAMP; and according to the concentration of IP1 and / or cAMP, determining whether the to-be-detected object is the substance inducing the anaphylactoid reaction or not. The detection method provided by the invention has the advantages that the result is accurate and reliable and is basically consistent with the detection result of the traditional beta-hexosaminidase release rate experiment, but the sensitivity is higher than that of a beta-hexosaminidase release rate detection method; and the detection method provided by the invention is simple and quick, overcomes the defects of the traditional method, can be applied to early stage rapid screening and identification of the substance inducing the anaphylactoid reaction and has extensive value in use.

Description

technical field [0001] The invention relates to the technical field of drug detection, in particular to a method for detecting substances that cause anaphylactoid reactions and a kit thereof. Background technique [0002] Allergic reaction is allergic reaction, which is an abnormal immune reaction between exogenous antigenic substances and antibodies in the body, and is one of the common adverse reactions. There are many substances that can induce allergic reactions, such as macromolecular substances such as proteins, polypeptides, and polysaccharides with complete antigenicity; other compounds with smaller molecules can be used as haptens to combine with proteins in the body to form complete antigens, thereby causing allergic reactions. At present, there are many allergic substances involved in allergic reactions, among which medicines (traditional Chinese medicine and western medicine), food, pollen, mites, etc. occur more frequently. Acute allergic reactions include type...

AI Technical Summary

Problems solved by technology

However, the calcium flow method is only suitable for the detection of adherent cells. If suspension or semi-suspension cells are used, the detection data will be unreal and unstable.

At the same time, calcium flux detection can only target compounds that quickly bind to receptors and exert their effects, and are not suitable for compounds that respond slowly

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